TDI-11904
TDI-11904 is a peripherally active EZH2 inhibitor with an EZH2 IC50 of 0.9 nM. TDI-11904 inhibits EZH2 methyltransferase activity, reduces the trimethylation level of H3K27me3, and decreases intracellular H3K27me3 content. TDI-11904 inhibits the proliferation of lymphoma cells. TDI-11904 can be used for the research of lymphoma.
For research use only. We do not sell to patients.
- CAS No.: 3056015-44-9
- Formula: C23H24Cl2N4O3S
- Molecular Weight:507.43
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
All Histone Methyltransferase Isoforms
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Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| KARPAS-422 | IC50 |
125 nM
Compound: 21
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Antiproliferative activity against human KARPAS-422 cells assessed as cell growth inhibition incubated for 72 hrs followed by compound dilution in growth medium and measured after 96 hrs by Alamar blue assay
Antiproliferative activity against human KARPAS-422 cells assessed as cell growth inhibition incubated for 72 hrs followed by compound dilution in growth medium and measured after 96 hrs by Alamar blue assay
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[PMID: 35300079] |
In Vitro
TDI-11904 inhibits H3K27me3 in Karpas-422 cells with an IC50 of 6.0 nM[1].
TDI-11904 inhibits Karpas-422 cell proliferation with an IC50 of 125 nM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 3056015-44-9
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Molecular Weight 507.43
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Formula C23H24Cl2N4O3S
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SMILES
O=C1NC(C)=CC(SC)=C1CN2C(C3=C(C(C4=C(C)ON=C4C)=CC(Cl)=C3N(CC2)C)Cl)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
TDI-11904 Related Classifications
HY-182448 Related Classifications
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