Thrombospondin (TSP-1)-derived CD36 binding motif
Based on 1 Customer Validation
Thrombospondin (TSP-1)-derived CD36 binding motif is a bioactive hexapeptide. Thrombospondin (TSP-1)-derived CD36 binding motif interferes with the interaction between cells and the extracellular matrix by binding to CD36 and angiostatin, thereby affecting the cell adhesion and migration process. Thrombospondin (TSP-1)-derived CD36 binding motif inhibits platelet aggregation. Thrombospondin (TSP-1)-derived CD36 binding motif exerts an anti-tumor effect against colon cancer.
For research use only. We do not sell to patients.
- Purity: 99.58%
- CAS No.: 138849-26-0
- Formula: C20H34N6O9S2
- Molecular Weight:566.65
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Thrombospondin (TSP-1)-derived CD36 binding motif (200 μM) inhibits thrombospondin surface expression on activated platelets and partially inhibits platelet aggregation in response to agonists such as A23187 (HY-N6687), Epinephrine (HY-B0447B), ADP (HY-W010918), and Collagen[1].
Thrombospondin (TSP-1)-derived CD36 binding motif (200 μM) completely inhibits the attachment of Retinoic acid (HY-14649)-treated SK-N-SH cells to TSP-1, with an IC50 of 80 μM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Female BALB/c nu/nu mice (weight: 12-18 g, age: 8-10 weeks), human xenograft splenic injection model of colon cancer[4]
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Dosage:10 mg/kg (in PBS)
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Administration:Intraperitoneal injection, every other day, 4 weeks
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Result:Significantly reduced the primary tumor volume by 69% compared with the control group.
Improved the health score and internal disease score, with a minimum 30% improvement in both scores compared with control animals.
Chemical Information
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CAS No. 138849-26-0
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Appearance Solid
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Molecular Weight 566.65
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Formula C20H34N6O9S2
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Color White to off-white
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Sequence
Cys-Ser-Val-Thr-Cys-Gly (Disulfide bridge: Cys1-Cys5)
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Sequence Shortening
CSVTCG (Disulfide bridge: Cys1-Cys5)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : 10 mg/mL (17.65 mM; Need ultrasonic)
DMSO : 10 mg/mL (17.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (284 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Asch AS, et al. Thrombospondin sequence motif (CSVTCG) is responsible for CD36 binding. Biochem Biophys Res Commun. 1992 Feb 14;182(3):1208-17. [Content Brief]
[2]. Pijuan-Thompson V, et al. Retinoic acid alters the mechanism of attachment of malignant astrocytoma and neuroblastoma cells to thrombospondin-1. Exp Cell Res. 1999 May 25;249(1):86-101. [Content Brief]
[3]. Tsao PW, et al. Thrombospondin mediates calcium mobilization in fibroblasts via its Arg-Gly-Asp and carboxyl-terminal domains. J Biol Chem. 1995 Oct 6;270(40):23747-53. [Content Brief]
[4]. Liebig C, et al. Angiocidin inhibitory peptides decrease tumor burden in a murine colon cancer model. J Surg Res. 2007 Oct;142(2):320-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.7648 mL | 8.8238 mL | 17.6476 mL | 44.1189 mL |
| 5 mM | 0.3530 mL | 1.7648 mL | 3.5295 mL | 8.8238 mL | |
| 10 mM | 0.1765 mL | 0.8824 mL | 1.7648 mL | 4.4119 mL | |
| 15 mM | 0.1177 mL | 0.5883 mL | 1.1765 mL | 2.9413 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Thrombospondin (TSP-1)-derived CD36 binding motif Related Classifications
HY-P5322 Related Classifications
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