Deoxythymidine-5'-triphosphate trisodium solution (100 mM)
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Deoxythymidine-5'-triphosphate trisodium solution (100 mM) (dTTP trisodium solution (100 mM)) is a deoxyribonucleoside triphosphate and a thymidine analog, which serves as a direct precursor for DNA synthesis. Deoxythymidine-5'-triphosphate trisodium solution (100 mM) can be used for DNA amplification in the DNA polymerase chain reaction (PCR). Deoxythymidine-5'-triphosphate trisodium solution (100 mM) is also applicable to research related to diseases such as leukemia.
For research use only. We do not sell to patients.
- Purity : 98.06%
- CAS No.: 27821-54-1
- Formula: C10H14N2Na3O14P3
- Molecular Weight:548.11
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Storage:
Solution, -20°C, 2 years
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Deoxythymidine-5'-triphosphateDeoxyribonucleoside Triphosphate
Biological Activity
Description
IC50 & Target
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Human Endogenous Metabolite |
In Vitro
Deoxythymidine‑5'-triphosphate (dTTP) shows an average concentration of 2.5 pmol/106 cells in resting human lymphocytes; dTTP concentration rises gradually during 24‑72 h culture of PHA‑stimulated human lymphocytes and reaches an average of 9.4 pmol/106 cells at 72 h[1].
Deoxythymidine‑5'-triphosphate exhibits an average concentration of 1.4 pmol/106 cells in normal bone marrow nucleated cells, while its average concentrations in bone marrow nucleated cells corresponding to acute myeloid leukemia, chronic myeloid leukemia, and myeloproliferative disorders are 5.8 pmol/106 cells, 3.8 pmol/106 cells, and 5.9 pmol/106 cells, respectively[1].
Deoxythymidine-5'-triphosphate trisodium solution (100 mM) can be used as a key variable in optimizing PCR amplification strategies for long-fragment, high A+T content mitochondrial DNA: increasing the concentrations of dATP and dTTP in the PCR reaction system while decreasing the concentrations of dGTP and dCTP[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 27821-54-1
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Appearance Liquid
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Molecular Weight 548.11
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Formula C10H14N2Na3O14P3
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Color Colorless to light yellow
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SMILES
O[C@H]([C@H](O1)COP(OP(OP(O)(O[Na])=O)(O[Na])=O)(O[Na])=O)C[C@@H]1N(C2=O)C=C(C(N2)=O)C
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Synonyms
dTTP trisodium solution (100 mM)
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Solution, -20°C, 2 years
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
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BrdU Incorporation Assay
Bromodeoxyuridine (BrdU) incorporation assay is based on the principle that BrdU, a thymidine analog, is incorporated into newly synthesized DNA during the S phase of the cell cycle, thereby serving as a marker of DNA replication and cellular proliferation. Incorporated BrdU can be detected using anti-BrdU antibodies following DNA denaturation, enabling visualization or quantification of proliferating cells through immunochemical detection methods such as immunofluorescence or immunohistochemistry.
Purity & Documentation
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Data Sheet (285 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Tattersall MH, et al. Deoxyribonucleoside triphosphates in human cells: changes in disease and following exposure to drugs. European journal of clinical investigation. 1975 Apr;5(2):191-202. [Content Brief]
[2]. Rondan Dueñas JC, et al. Specific requirements for PCR amplification of long mitochondrial A+T-rich DNA. Biotechniques. 1999 Aug;27(2):258-60. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Deoxythymidine-5'-triphosphate trisodium solution (100 mM) Related Classifications
HY-W013715A Related Classifications
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Keywords
- Deoxythymidine-5'-triphosphate trisodium solution (100 mM)
- 27821-54-1
- dTTP trisodium solution (100 mM)
- DNA/RNA Synthesis
- Nucleoside Antimetabolite/Analog
- Endogenous Metabolite
- chronic granulocytic leukaemia
- acute myeloid leukaemia
- DNA synthesis
- phytohaemagglutinin-stimulated lymphocytes
- leukaemia
- antifolate agents
- nucleated marrow cells
- hydroxyurea
- 5-fluorouracil
- myeloproliferative diseases
- Inhibitor
- inhibitor
- inhibit