Deoxythymidine-5'-triphosphate
Deoxythymidine-5'-triphosphate (dTTP) is a deoxyribonucleoside triphosphate and a thymidine analog, which serves as a direct precursor for DNA synthesis. Deoxythymidine-5'-triphosphate can be used for DNA amplification in the DNA polymerase chain reaction (PCR). Deoxythymidine-5'-triphosphate is also applicable to research related to diseases such as leukemia.
For research use only. We do not sell to patients.
- CAS No.: 365-08-2
- Formula: C10H17N2O14P3
- Molecular Weight:482.17
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Human Endogenous Metabolite |
Deoxythymidine‑5'-triphosphate (dTTP) shows an average concentration of 2.5 pmol/106 cells in resting human lymphocytes; dTTP concentration rises gradually during 24‑72 h culture of PHA‑stimulated human lymphocytes and reaches an average of 9.4 pmol/106 cells at 72 h[1].
Deoxythymidine‑5'-triphosphate exhibits an average concentration of 1.4 pmol/106 cells in normal bone marrow nucleated cells, while its average concentrations in bone marrow nucleated cells corresponding to acute myeloid leukemia, chronic myeloid leukemia, and myeloproliferative disorders are 5.8 pmol/106 cells, 3.8 pmol/106 cells, and 5.9 pmol/106 cells, respectively[1].
Deoxythymidine-5'-triphosphate can be used as a key variable in optimizing PCR amplification strategies for long-fragment, high A+T content mitochondrial DNA: increasing the concentrations of dATP and dTTP in the PCR reaction system while decreasing the concentrations of dGTP and dCTP[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 365-08-2
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Molecular Weight 482.17
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Formula C10H17N2O14P3
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SMILES
O=C1N([C@H]2C[C@H](O)[C@@H](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)O2)C=C(C(N1)=O)C
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Synonyms
dTTP
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Tattersall MH, et al. Deoxyribonucleoside triphosphates in human cells: changes in disease and following exposure to drugs. European journal of clinical investigation. 1975 Apr;5(2):191-202. [Content Brief]
[2]. Rondan Dueñas JC, et al. Specific requirements for PCR amplification of long mitochondrial A+T-rich DNA. Biotechniques. 1999 Aug;27(2):258-60. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Deoxythymidine-5'-triphosphate Related Classifications
HY-138615 Related Classifications
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- Deoxythymidine-5'-triphosphate
- 365-08-2
- dTTP
- DNA/RNA Synthesis
- Nucleoside Antimetabolite/Analog
- Endogenous Metabolite
- chronic granulocytic leukaemia
- acute myeloid leukaemia
- DNA synthesis
- phytohaemagglutinin-stimulated lymphocytes
- leukaemia
- antifolate agents
- nucleated marrow cells
- hydroxyurea
- 5-fluorouracil
- myeloproliferative diseases
- Inhibitor
- inhibitor
- inhibit