Referencia número: HY-13543

Tretazicar

Name: Tretazicar
Brand: MedChemExpress
SKU: HY-13543
Price: 60.00 USD
Availability: InStock
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(Synonyms: CB 1954)

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Tretazicar (CB 1954), an antitumor proagent, is highly selective against the Walker 256 rat tumour line. Tretazicar is enzymatically activated to generate a bifunctional agent, which can form DNA-DNA interstrand cross-links. Tretazicar in rat cells involves the reduction of its 4-nitro group to a 4-hydroxylamine by the enzyme NAD(P)H:quinone oxidoreductase 1 (NQO1).

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Pureza : 99.65%
No. CAS: 21919-05-1
Fòrmula: C₉H₈N₄O₅
Peso molecular:252.18

Almacenamiento:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

Tretazicar Chemical Structure

No. CAS: 21919-05-1

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Actividad biológica
- Descripciòn
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvente y solubilidad
- In Vitro
- In Vivo
Pureza y Documentación
Referencias
Help & FAQs
Classifications
Bioz

Actividad biológica

Descripciòn

Cellular Effect

Cell Line	Type	Value	Description	References
CHO-AA8	IC₅₀	560 μM Compound: 23	Growth inhibition of aerobic chinese hamster ovary fibroblast AA8 cell line using an exposure time of 18 hours Growth inhibition of aerobic chinese hamster ovary fibroblast AA8 cell line using an exposure time of 18 hours	[PMID: 1507207]
CHO-AA8	IC₅₀	563 μM Compound: 7	Aerobic cytotoxicity was assessed in a growth inhibition assay using log phase cultures of the chinese hamster ovary fibroblast line AA8 Aerobic cytotoxicity was assessed in a growth inhibition assay using log phase cultures of the chinese hamster ovary fibroblast line AA8	[PMID: 8035424]
EMT6	IC₅₀	71 μM Compound: 1	Inhibitory activity against EMT6 (mouse mammary carcinoma) murine cell line Inhibitory activity against EMT6 (mouse mammary carcinoma) murine cell line	[PMID: 14640560]
EMT6	IC₅₀	0.098 μM Compound: 1	Growth inhibitory potency against NTR-Transfected (nitroreductase) mouse mammary cell line known as EMT6-NTRpuro cell line Growth inhibitory potency against NTR-Transfected (nitroreductase) mouse mammary cell line known as EMT6-NTRpuro cell line	[PMID: 15163209]
EMT6	IC₅₀	0.098 μM Compound: 1, CB-1954	Antiproliferative activity against mouse EMT6 NTRpuro cells after 18 hrs Antiproliferative activity against mouse EMT6 NTRpuro cells after 18 hrs	[PMID: 17326614]
EMT6	IC₅₀	83 μM Compound: 1, CB-1954	Antiproliferative activity against mouse wild type EMT6 NTR-ve cells after 18 hrs Antiproliferative activity against mouse wild type EMT6 NTR-ve cells after 18 hrs	[PMID: 17326614]
Mammary carcinoma cell line	IC₅₀	710 μM Compound: 1	Cytotoxicity in non-NTR-expressing mouse mammary carcinoma cell line (EMT6). Cytotoxicity in non-NTR-expressing mouse mammary carcinoma cell line (EMT6).	[PMID: 12773049]
Ovarian cancer cell line	IC₅₀	174 μM Compound: 1	Cytotoxicity in non-NTR-expressing human ovarian carcinoma cell line (SKOV). Cytotoxicity in non-NTR-expressing human ovarian carcinoma cell line (SKOV).	[PMID: 12773049]
PC-3	IC₅₀	1.792 nM Compound: CB1954	Cytotoxicity against human PC3 cells assessed as reduction in cell viability measured after 48 hrs in presence of Staphylococcus saprophyticus nitroreductase and NADH by SRB assay Cytotoxicity against human PC3 cells assessed as reduction in cell viability measured after 48 hrs in presence of Staphylococcus saprophyticus nitroreductase and NADH by SRB assay	[PMID: 30928710]
PC-3	IC₅₀	1.792 nM Compound: CB1954	Cytotoxicity against human PC3 cells expressing Staphylococcus saprophyticus nitroreductase NtrB assessed as reduction in cell viability in presence of NADH as cofactor after 48 hrs by SRB assay Cytotoxicity against human PC3 cells expressing Staphylococcus saprophyticus nitroreductase NtrB assessed as reduction in cell viability in presence of NADH as cofactor after 48 hrs by SRB assay	[PMID: 31841727]
RT-112	IC₅₀	0.15 μM Compound: CB-1954	Cytotoxicity against NQO2 expressing human RT112 cells in presence of NRH cofactor by MTT assay Cytotoxicity against NQO2 expressing human RT112 cells in presence of NRH cofactor by MTT assay	[PMID: 17643990]
RT-112	IC₅₀	88.75 μM Compound: CB-1954	Cytotoxicity against NQO2 expressing human RT112 cells by MTT assay Cytotoxicity against NQO2 expressing human RT112 cells by MTT assay	[PMID: 17643990]
SK-OV-3	IC₅₀	0.76 μM Compound: 7	In vitro for cytotoxicity against SKOV-3 cells in the presence of 1 mM NADH co-factor In vitro for cytotoxicity against SKOV-3 cells in the presence of 1 mM NADH co-factor	[PMID: 10617083]
SK-OV-3	IC₅₀	1.01 μM Compound: 7	In vitro for cytotoxicity against SKOV-3 cells in the presence of 1 mM NADH co-factor and 1 ug/mL Escherichia coli B nitroreductase (NTR) In vitro for cytotoxicity against SKOV-3 cells in the presence of 1 mM NADH co-factor and 1 ug/mL Escherichia coli B nitroreductase (NTR)	[PMID: 10617083]
SK-OV-3	IC₅₀	182 μM Compound: 7	In vitro for cytotoxicity against SKOV-3 cells in the absence of co-factor In vitro for cytotoxicity against SKOV-3 cells in the absence of co-factor	[PMID: 10617083]
SK-OV-3	IC₅₀	4.7 μM Compound: 1	Inhibitory activity against SKOV3 (human ovarian carcinoma) cell line with Escherichia coli nitroreductase combination after 18 hours drug exposure Inhibitory activity against SKOV3 (human ovarian carcinoma) cell line with Escherichia coli nitroreductase combination after 18 hours drug exposure	[PMID: 14584930]
SK-OV-3	IC₅₀	52 μM Compound: 1	Inhibitory activity against SKOV3 (human ovarian carcinoma) cell line infected at lower infection rate of 10 pfu/cell with Escherichia coli nitroreductase combination after 18 hours drug exposure Inhibitory activity against SKOV3 (human ovarian carcinoma) cell line infected at lower infection rate of 10 pfu/cell with Escherichia coli nitroreductase combination after 18 hours drug exposure	[PMID: 14584930]
SK-OV-3	IC₅₀	174 μM Compound: 1	Inhibitory activity against SKOV3 (human ovarian carcinoma) cell line in human Inhibitory activity against SKOV3 (human ovarian carcinoma) cell line in human	[PMID: 14640560]
SK-OV-3	IC₅₀	0.64 μM Compound: 1	Growth inhibitory potency against NTR-Transfected (nitroreductase) human ovarian cell line known as SKOV3-NTR neo cell line Growth inhibitory potency against NTR-Transfected (nitroreductase) human ovarian cell line known as SKOV3-NTR neo cell line	[PMID: 15163209]
SK-OV-3	IC₅₀	>790 μM Compound: 5-aziridinyl-2,4-dinitrobenzamide	Antiproliferative activity against human SKOV3 cells after 18 hrs Antiproliferative activity against human SKOV3 cells after 18 hrs	[PMID: 16821793]
SK-OV-3	IC₅₀	2 μM Compound: 5-aziridinyl-2,4-dinitrobenzamide	Antiproliferative activity against NTR expressing human SKOV3 cells after 18 hrs Antiproliferative activity against NTR expressing human SKOV3 cells after 18 hrs	[PMID: 16821793]
SK-OV-3	IC₅₀	4.7 μM Compound: 5-aziridinyl-2,4-dinitrobenzamide	Antiproliferative activity against NTR expressing human SKOV3 cells infected with 100 pfu/cell retrovirus after 18 hrs Antiproliferative activity against NTR expressing human SKOV3 cells infected with 100 pfu/cell retrovirus after 18 hrs	[PMID: 16821793]
SK-OV-3	IC₅₀	52 μM Compound: 5-aziridinyl-2,4-dinitrobenzamide	Antiproliferative activity against NTR expressing human SKOV3 cells infected with 10 pfu/cell retrovirus after 18 hrs Antiproliferative activity against NTR expressing human SKOV3 cells infected with 10 pfu/cell retrovirus after 18 hrs	[PMID: 16821793]
SK-OV-3	IC₅₀	625 μM Compound: 5-aziridinyl-2,4-dinitrobenzamide	Antiproliferative activity against human SKOV3 cells infected with 10 pfu/cell retrovirus after 18 hrs Antiproliferative activity against human SKOV3 cells infected with 10 pfu/cell retrovirus after 18 hrs	[PMID: 16821793]
SK-OV-3	IC₅₀	>1000 μM Compound: 5-aziridinyl-2,4-dinitrobenzamide	Antiproliferative activity against human SKOV3 cells infected with 100 pfu/cell retrovirus after 18 hrs Antiproliferative activity against human SKOV3 cells infected with 100 pfu/cell retrovirus after 18 hrs	[PMID: 16821793]
T78-1	IC₅₀	395 μM Compound: 7	In vitro cytotoxicity against Chinese hamster V79-derived cell line T78-1 transfected with Escherichia coli B nitroreductase (NTR) In vitro cytotoxicity against Chinese hamster V79-derived cell line T78-1 transfected with Escherichia coli B nitroreductase (NTR)	[PMID: 10617083]
T78-1	IC₅₀	158 μM Compound: 1	Cytotoxicity in T78-1 (NR-negative) cell lines Cytotoxicity in T78-1 (NR-negative) cell lines	[PMID: 9111301]
UV4	IC₅₀	11.7 μM Compound: 23	Growth inhibition of aerobic chinese hamster ovary fibroblast UV4 cells using an exposure time of 18 hours Growth inhibition of aerobic chinese hamster ovary fibroblast UV4 cells using an exposure time of 18 hours	[PMID: 1507207]
V79	IC₅₀	374 μM Compound: 1	Cytotoxicity in non-NTR-expressing Chinese hamster fibroblast (V79). Cytotoxicity in non-NTR-expressing Chinese hamster fibroblast (V79).	[PMID: 12773049]
V79	IC₅₀	>100 μM Compound: 1	Inhibitory activity against V79 (chinese hamster fibroblast) cell line without Escherichia coli nitroreductase combination after 1 hour drug exposure Inhibitory activity against V79 (chinese hamster fibroblast) cell line without Escherichia coli nitroreductase combination after 1 hour drug exposure	[PMID: 14584930]
V79	IC₅₀	0.036 μM Compound: 1	Inhibitory activity against V79 (chinese hamster fibroblast) cell line with Escherichia coli nitroreductase combination after 72 hours drug exposure Inhibitory activity against V79 (chinese hamster fibroblast) cell line with Escherichia coli nitroreductase combination after 72 hours drug exposure	[PMID: 14584930]
V79	IC₅₀	0.31 μM Compound: 1	Inhibitory activity against V79 (chinese hamster fibroblast) cell line with Escherichia coli nitroreductase combination after 1 hour drug exposure Inhibitory activity against V79 (chinese hamster fibroblast) cell line with Escherichia coli nitroreductase combination after 1 hour drug exposure	[PMID: 14584930]
V79	IC₅₀	254 μM Compound: 1	Inhibitory activity against V79 (chinese hamster fibroblast) cell line without Escherichia coli nitroreductase combination after 72 hours drug exposure Inhibitory activity against V79 (chinese hamster fibroblast) cell line without Escherichia coli nitroreductase combination after 72 hours drug exposure	[PMID: 14584930]
V79	IC₅₀	374 μM Compound: 1	Inhibitory activity against V79 (Chinese hamster fibroblast) cell line in chinese hamster Inhibitory activity against V79 (Chinese hamster fibroblast) cell line in chinese hamster	[PMID: 14640560]
V79	IC₅₀	0.26 μM Compound: 1	Growth inhibitory potency against NTR-Transfected (nitroreductase) chinese hamster fibroblast known as V79-NTRpuro cell line Growth inhibitory potency against NTR-Transfected (nitroreductase) chinese hamster fibroblast known as V79-NTRpuro cell line	[PMID: 15163209]
V79	IC₅₀	>100 μM Compound: 5-aziridinyl-2,4-dinitrobenzamide	Antiproliferative activity against chinese hamster V79 cells after 1 hr Antiproliferative activity against chinese hamster V79 cells after 1 hr	[PMID: 16821793]
V79	IC₅₀	0.036 μM Compound: 5-aziridinyl-2,4-dinitrobenzamide	Antiproliferative activity against NTR expressing chinese hamster V79 cells after 72 hrs Antiproliferative activity against NTR expressing chinese hamster V79 cells after 72 hrs	[PMID: 16821793]
V79	IC₅₀	0.31 μM Compound: 5-aziridinyl-2,4-dinitrobenzamide	Antiproliferative activity against NTR expressing chinese hamster V79 cells after 1 hr Antiproliferative activity against NTR expressing chinese hamster V79 cells after 1 hr	[PMID: 16821793]
V79	IC₅₀	254 μM Compound: 5-aziridinyl-2,4-dinitrobenzamide	Antiproliferative activity against chinese hamster V79 cells after 72 hrs Antiproliferative activity against chinese hamster V79 cells after 72 hrs	[PMID: 16821793]
V79	IC₅₀	0.26 μM Compound: 1, CB-1954	Antiproliferative activity against chinese hamster V79 NTRpuro cells after 18 hrs Antiproliferative activity against chinese hamster V79 NTRpuro cells after 18 hrs	[PMID: 17326614]
V79	IC₅₀	374 μM Compound: 1, CB-1954	Antiproliferative activity against chinese hamster wild-type V79 NTR-ve cells after 18 hrs Antiproliferative activity against chinese hamster wild-type V79 NTR-ve cells after 18 hrs	[PMID: 17326614]
V79	IC₅₀	183 μM Compound: CB-1954	Therapeutic index, ratio of IC50 for chinese hamster V79 cells to IC50 for Trypanosoma brucei BSF Therapeutic index, ratio of IC50 for chinese hamster V79 cells to IC50 for Trypanosoma brucei BSF	[PMID: 20679506]
V79	IC₅₀	543 μM Compound: CB-1954	Cytotoxicity against chinese hamster V79 cells after 6 days by Alamar blue assay Cytotoxicity against chinese hamster V79 cells after 6 days by Alamar blue assay	[PMID: 20679506]
V79	IC₅₀	953 μM Compound: CB-1954	Therapeutic index, ratio of IC50 for chinese hamster V79 cells to IC50 for Trypanosoma cruzi Therapeutic index, ratio of IC50 for chinese hamster V79 cells to IC50 for Trypanosoma cruzi	[PMID: 20679506]
Vero	IC₅₀	>250 μM Compound: CB-1954	Cytotoxicity against african green monkey Vero cells after 6 days by Alamar blue assay Cytotoxicity against african green monkey Vero cells after 6 days by Alamar blue assay	[PMID: 20679506]
Vero	IC₅₀	0.57 μM Compound: CB-1954	Antitrypanosomal activity against Trypanosoma cruzi clone Cl-Brener infected in african green monkey Vero cells assessed as growth inhibition after 3 days by luciferase reporter gene assay Antitrypanosomal activity against Trypanosoma cruzi clone Cl-Brener infected in african green monkey Vero cells assessed as growth inhibition after 3 days by luciferase reporter gene assay	[PMID: 20679506]
WiDr	IC₅₀	565.4 μM Compound: 7	In vitro cytotoxicity against man colon carcinoma line WiDr cell lines transfected with Escherichia coli B nitroreductase (NTR) In vitro cytotoxicity against man colon carcinoma line WiDr cell lines transfected with Escherichia coli B nitroreductase (NTR)	[PMID: 10617083]
WiDr	IC₅₀	54 μM Compound: 1	Cytotoxicity in non-NTR-expressing human colon carcinoma cell line (WiDr). Cytotoxicity in non-NTR-expressing human colon carcinoma cell line (WiDr).	[PMID: 12773049]
WiDr	IC₅₀	54 μM Compound: 1	Inhibitory activity against WiDr (human colon carcinoma) cell line in human Inhibitory activity against WiDr (human colon carcinoma) cell line in human	[PMID: 14640560]
WiDr	IC₅₀	1.14 μM Compound: 1, CB-1954	Antiproliferative activity against human WiDr NTRneo cells after 18 hrs Antiproliferative activity against human WiDr NTRneo cells after 18 hrs	[PMID: 17326614]
WiDr	IC₅₀	54.5 μM Compound: 1, CB-1954	Antiproliferative activity against human wild type WiDr NTR-ve cells after 18 hrs Antiproliferative activity against human wild type WiDr NTR-ve cells after 18 hrs	[PMID: 17326614]
WiDr-NTR	IC₅₀	1.14 μM Compound: 1	Growth inhibitory potency against NTR-Transfected (nitroreductase) human colon cell line known as WiDr-NTRneo cell line Growth inhibitory potency against NTR-Transfected (nitroreductase) human colon cell line known as WiDr-NTRneo cell line	[PMID: 15163209]

In Vitro

Tretazicar (CB 1954) (0.1-1000 μM; 3 days) has sensitivity for retrovirally transduced AB22 (AB22-nr) cells with an IC₅₀ of 3 μM^[3].
DNA cross-link formation in affected cells is a result of the bioactivation of the drug by the enzyme DT diaphorase (NAD(P)H dehydro-genase (quinone)) in the Walker cells which reduces the 4-nitro group of Tretazicar. The product of this reaction is a difunctional alkylating agent, 5-aziridin-1-yl-4-hydroxylamino-2-nitrobenzamide^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .

In Vivo

Tretazicar (CB 1954) (80 mg/kg; i.p. on days 2 and 9) results in a significant increase in survival^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Female BALB/c mice (AB22-nr, SKOV3 human ovarian tumour xenograft)^[3]
Dosage:

80 mg/kg
Administration:

i.p. on days 2 and 9
Result:

The median survival of the AB22-nr was 49 days. Resulted in a significant increase in survival.

Chemical Information

No. CAS 21919-05-1
Appearance Solid
Peso molecular 252.18
Fòrmula C₉H₈N₄O₅
Color Light yellow to yellow
SMILES

O=C(N)C1=CC(N2CC2)=C([N+]([O-])=O)C=C1[N+]([O-])=O
Synonyms

CB 1954
Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvente y solubilidad

In Vitro:

DMSO : 125 mg/mL (495.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.9654 mL

19.8271 mL

39.6542 mL

5 mM

0.7931 mL

3.9654 mL

7.9308 mL

10 mM

0.3965 mL

1.9827 mL

3.9654 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (8.25 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (8.25 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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g
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
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+
%
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The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (278 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Knox RJ,et al. Bioactivation of 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB 1954) by human NAD(P)H quinone oxidoreductase 2: a novel co-substrate-mediated antitumor prodrug therapy. Cancer Res. 2000 Aug 1;60(15):4179-86. [Content Brief]

[2]. Knox RJ, et al. CB 1954: from the Walker tumor to NQO2 and VDEPT. Curr Pharm Des. 2003;9(26):2091-104. [Content Brief]

[3]. Green NK, et al. Immune enhancement of nitroreductase-induced cytotoxicity: studies using a bicistronicadenovirus vector. Int J Cancer. 2003 Mar 10;104(1):104-12. [Content Brief]

[4]. Drabek D, et al. The expression of bacterial nitroreductase in transgenic mice results in specific cell killing by the prodrug CB1954. Gene Ther. 1997 Feb;4(2):93-100. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.9654 mL	19.8271 mL	39.6542 mL	99.1355 mL
	5 mM	0.7931 mL	3.9654 mL	7.9308 mL	19.8271 mL
	10 mM	0.3965 mL	1.9827 mL	3.9654 mL	9.9136 mL
	15 mM	0.2644 mL	1.3218 mL	2.6436 mL	6.6090 mL
	20 mM	0.1983 mL	0.9914 mL	1.9827 mL	4.9568 mL
	25 mM	0.1586 mL	0.7931 mL	1.5862 mL	3.9654 mL
	30 mM	0.1322 mL	0.6609 mL	1.3218 mL	3.3045 mL
	40 mM	0.0991 mL	0.4957 mL	0.9914 mL	2.4784 mL
	50 mM	0.0793 mL	0.3965 mL	0.7931 mL	1.9827 mL
	60 mM	0.0661 mL	0.3305 mL	0.6609 mL	1.6523 mL
	80 mM	0.0496 mL	0.2478 mL	0.4957 mL	1.2392 mL
	100 mM	0.0397 mL	0.1983 mL	0.3965 mL	0.9914 mL

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Tretazicar