Uralione D
Uralione D is a neuroprotective agent found in Hypericum uralum and Hypericum kouytchense. Uralione D exhibits cytotoxic activity against gastric cancer cells and protects against Corticosterone (HY-B1618)-induced damage in neuron-like cells. Uralione D is useful for research on depression, multidrug-resistant liver cancer, and gastric cancer.
For research use only. We do not sell to patients.
- CAS No.: 1927024-52-9
- Formula: C38H52O7
- Molecular Weight:620.82
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MKN-45 | IC50 |
18.47 μM
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Cytotoxicity against human MKN-45 gastric cancer cells assessed as reduction in cell viability incubated for 24 h by Cell Counting Kit-8 (CCK-8) assay.
Cytotoxicity against human MKN-45 gastric cancer cells assessed as reduction in cell viability incubated for 24 h by Cell Counting Kit-8 (CCK-8) assay.
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slct.202504003 |
In Vitro
Uralione D (compound 4) (0.1-10.0 μM; 48 h) protects PC12 cells from Corticosterone (HY-B1618)-induced injury, with cell viabilities ranging from 72.5% to 80.9% across 0.1-10.0 μM concentrations after 48 h of co-incubation[1].
Uralione D (compound 5) (24 h) exerts potent cytotoxicity against human MKN-45 gastric cancer cells, with an IC50 value of 18.47 µM after 24 h of treatment[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:PC12 cells
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Concentration:0.1, 0.3, 1.0, 3.0, 10.0 μM
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Incubation Time:48 h
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Result:Exhibited significant protective effects against corticosterone-induced PC12 cell injury at all tested concentrations.
Showed cell viability of 77.9% at 0.1 μM relative to untreated control set at 100%.
Showed cell viability of 77.7% at 0.3 μM relative to untreated control set at 100%.
Showed cell viability of 78.5% at 1.0 μM relative to untreated control set at 100%.
Showed cell viability of 72.5% at 3.0 μM relative to untreated control set at 100%.
Showed cell viability of 80.9% at 10.0 μM relative to untreated control set at 100%.
Achieved the highest viability at 10.0 μM.
Chemical Information
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CAS No. 1927024-52-9
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Molecular Weight 620.82
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Formula C38H52O7
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SMILES
O=C(C1=CC=CC=C1)[C@]23C4=C(C[C@@H](C(C)(O4)C)O)C([C@](C2=O)(C[C@@H]([C@@]3(C)CC/C=C(C)/C)C/C=C(C)\C)C[C@H](O)C(C)(O)C)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Liver Cancer Modeling
Liver cancer can be classified into primary liver cancer and secondary liver cancer. Secondary liver cancer is the metastatic liver cancer. Primary liver cancer includes hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma (ICC) and fibrolamellar HCC, of which HCC is the most common form, accounting for approximately 90% of primary liver cancers[1]. HCC mouse models include chemical agent-induced models, transplanted tumor models, and genetic engineered models.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Uralione D Related Classifications
HY-N21685 Related Classifications
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Keywords
- Uralione D
- 1927024-52-9
- Endogenous Metabolite
- MKN-45 gastric cancer cells
- Hypericum kouytchense
- multidrug-resistant hepatocellular carcinoma cells
- Hypericum uralum
- corticosterone-induced injury
- depression
- PC12 cells
- polycyclic polyprenylated acylphloroglucinol
- gastric cancer cells
- neuronal-like cells
- Inhibitor
- inhibitor
- inhibit