Tetrahydroxyquinone
Based on 1 publication(s) in Google Scholar
Tetrahydroxyquinone (Tetrahydroxy-1,4-benzoquinone), a primitive anticataract agent, is a redox active benzoquinone. Tetrahydroxyquinone can take part in a redox cycle with semiquinone radicals, leading to the formation of reactive oxygen species (ROS).
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 319-89-1
- Formula: C6H4O6
- Molecular Weight:172.09
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Tetrahydroxyquinone
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Biological Activity
Tetrahydroxyquinone (100-500 μM; 24 hours; HL60 cells) treatment shows cytotoxic for HL60 leukaemia cells by total protein content (IC50 of 20 µM), phosphatase activity (IC50 of 40 µM), or by MTT assay (IC50 of 45 µM). Tetrahydroxyquinone is an efficient inducer of ROS production in HL60 leukaemia cells[1].
Tetrahydroxyquinone efficiently activates caspase 3 in concentration in excess of 25 µM, stimulates DNA fragmentation at the same concentration and provoke phosphatidylserine exposure[1].
Tetrahydroxyquinone induces the release of cytochrome c from the mitochondria at concentration as low as 25 µM. Tetrahydroxyquinone treatment also causes increase of phosphorylation of Ser473 in protein kinase B (the Bad kinase for Ser112)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HL60 leukaemia cells
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Concentration:100 μM, 200 μM, 300 μM, 400 μM, 500 μM
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Incubation Time:24 hours
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Result:Showed cytotoxic for HL60 leukaemia cells.
Chemical Information
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CAS No. 319-89-1
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Appearance Solid
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Molecular Weight 172.09
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Formula C6H4O6
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Color Light brown to black
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SMILES
O=C1C(O)=C(O)C(C(O)=C1O)=O
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Synonyms
Tetrahydroxy-1,4-benzoquinone; Tetrahydroxybenzoquinone
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (1)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
2026 Mar;13(16):e13310. PMID: 41589654
Solvent & Solubility
DMSO : 100 mg/mL (581.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.53 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.53 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.8109 mL | 29.0546 mL | 58.1091 mL | 145.2728 mL |
| 5 mM | 1.1622 mL | 5.8109 mL | 11.6218 mL | 29.0546 mL | |
| 10 mM | 0.5811 mL | 2.9055 mL | 5.8109 mL | 14.5273 mL | |
| 15 mM | 0.3874 mL | 1.9370 mL | 3.8739 mL | 9.6849 mL | |
| 20 mM | 0.2905 mL | 1.4527 mL | 2.9055 mL | 7.2636 mL | |
| 25 mM | 0.2324 mL | 1.1622 mL | 2.3244 mL | 5.8109 mL | |
| 30 mM | 0.1937 mL | 0.9685 mL | 1.9370 mL | 4.8424 mL | |
| 40 mM | 0.1453 mL | 0.7264 mL | 1.4527 mL | 3.6318 mL | |
| 50 mM | 0.1162 mL | 0.5811 mL | 1.1622 mL | 2.9055 mL | |
| 60 mM | 0.0968 mL | 0.4842 mL | 0.9685 mL | 2.4212 mL | |
| 80 mM | 0.0726 mL | 0.3632 mL | 0.7264 mL | 1.8159 mL | |
| 100 mM | 0.0581 mL | 0.2905 mL | 0.5811 mL | 1.4527 mL |
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