V023-9340

Cat. No.: HY-163071 Purity: 99.83%: Data Sheet
COA

SDS
Handling Instructions
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V023-9340 is a potent FXR inhibitor with IC₅₀ of 4.27 μM that can be used in NASH (nonalcoholic steatohepatitis) research.

For research use only. We do not sell to patients.

V023-9340 Chemical Structure

CAS No. : 1048304-96-6

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Description

V023-9340 is a potent FXR inhibitor with IC₅₀ of 4.27 μM that can be used in NASH (nonalcoholic steatohepatitis) research^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
HepG2	IC₅₀	> 100 μM Compound: V023-9340	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 38043493]
L02	IC₅₀	> 100 μM Compound: V023-9340	Cytotoxicity human L02 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity human L02 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 38043493]

Molecular Weight

484.59

Formula

C₃₀H₃₂N₂O₄

CAS No.

1048304-96-6

Appearance

Solid

Color

White to off-white

SMILES

O=C(NCC1=CC=C2OCOC2=C1)CC3=CC=C(C=C3)N(C(C4CCCC4)=O)CC5=CC=CC(C)=C5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (206.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0636 mL	10.3180 mL	20.6360 mL
5 mM	0.4127 mL	2.0636 mL	4.1272 mL
10 mM	0.2064 mL	1.0318 mL	2.0636 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.16 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.16 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.83%

Select Batch:

Data Sheet (270 KB) SDS (251 KB)

COA (248 KB) HNMR (400 KB) RP-HPLC (261 KB) LCMS (99 KB)

Handling Instructions (2659 KB)

References

[1]. Cong Chen, et al. Discovery of 4-aminophenylacetamide derivatives as intestine-specific farnesoid X receptor antagonists for the potential treatment of nonalcoholic steatohepatitis. Eur J Med Chem. 2023 Nov 27:264:115992. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0636 mL	10.3180 mL	20.6360 mL	51.5900 mL
	5 mM	0.4127 mL	2.0636 mL	4.1272 mL	10.3180 mL
	10 mM	0.2064 mL	1.0318 mL	2.0636 mL	5.1590 mL
	15 mM	0.1376 mL	0.6879 mL	1.3757 mL	3.4393 mL
	20 mM	0.1032 mL	0.5159 mL	1.0318 mL	2.5795 mL
	25 mM	0.0825 mL	0.4127 mL	0.8254 mL	2.0636 mL
	30 mM	0.0688 mL	0.3439 mL	0.6879 mL	1.7197 mL
	40 mM	0.0516 mL	0.2580 mL	0.5159 mL	1.2898 mL
	50 mM	0.0413 mL	0.2064 mL	0.4127 mL	1.0318 mL
	60 mM	0.0344 mL	0.1720 mL	0.3439 mL	0.8598 mL
	80 mM	0.0258 mL	0.1290 mL	0.2580 mL	0.6449 mL
	100 mM	0.0206 mL	0.1032 mL	0.2064 mL	0.5159 mL

V023-9340 Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

V023-93401048304-96-6FXRNR1H4HFD-induced NASHhepatic FXRInhibitorinhibitorinhibit

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V023-9340

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