Vari Fluor 480 TSA, 200× (same as mIHC kit)
Based on 1 Customer Validation
Vari Fluor 480 TSA, 200× (same as mIHC kit) (VF 480 mIHC) is suitable for multicolor immunohistochemistry experiments and are compatible with the dyes in multicolor immunohistochemistry kits (Ex/Em = 450/480 nm).
For research use only. We do not sell to patients.
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
- Purity & Documentation
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Help & FAQs
Biological Activity
Remove the dye and dilute it using a dedicated dye diluent Tyramide Amplification Buffer (HY-D1840) (sold separately). The TSA fluorescent dye working solution is prepared as follows: VF fluorescent dye + TSA buffer. The recommended dilution ratio for the fluorescent dye and TSA buffer is 1:200. The dilution ratio can be flexibly adjusted and optimized based on specific conditions; the optimal range is 1:50 to 1:400; Generally, if the primary antibody incubation time is 1-3 hours at room temperature, a dilution ratio of 1:50-1:200 is recommended; if the primary antibody is incubated overnight at 4°C (12 hours or longer), a dilution ratio of 1:200-1:400 or higher is recommended.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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SMILES
[Vari Fluor 480 TSA, 200?(same as mIHC kit)]
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Synonyms
VF 480 mIHC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Purity & Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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