VH9
VH9 is a Free radical scavenger. VH9 binds to the active pocket of Keap1, blocks the interaction between Keap1 and Nrf2, and activates the Keap1-Nrf2 antioxidant pathway. VH9 inhibits ROS production. VH9 scavenges ABTS and DPPH free radicals through hydrogen bonding and hydrophobic interactions. VH9 protects cells from oxidative stress damage.
For research use only. We do not sell to patients.
- Formula: C48H63N11O17
- Molecular Weight:1066.08
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
VH9 exhibits potent ABTS radical scavenging activity in a cell-free assay, with an IC50 of 19.698 μM[1].
VH9 exhibits DPPH radical scavenging activity in a cell-free assay, with an IC50 of 1500.825 μM[1].
VH9 (0.1-3 mg/mL; 1-2 h) exhibits good stability across varying temperatures, pH levels, NaCl concentrations, and simulated gastrointestinal digestion, retaining significant radical scavenging activity under all tested conditions[1].
VH9 (62.5-1500 μg/mL; 24 h) protects human HepG2 cells from H2O2-induced oxidative stress, increasing cell viability to up to 84.52% at a concentration of 1500 μg/mL[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:human HepG2 cells
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Concentration:62.5-1500 μg/mL (VH9 pre-incubation); 800 μM (H2O2 incubation)
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Incubation Time:24 h (VH9 pre-incubation); 2 h (H2O2 incubation)
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Result:Significantly improved the viability of H2O2-damaged HepG2 cells, with cell viability reaching 65.67%, 80.39%, and 84.52% at concentrations of 500, 1000, and 1500 μg/mL, respectively.
Showed greater protective potency than FK10 at all tested concentrations.
Chemical Information
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Molecular Weight 1066.08
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Formula C48H63N11O17
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Sequence
Val-Gly-Ser-Tyr-Glu-Asp-Pro-Tyr-His
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Sequence Shortening
VGSYEDPYH
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
VH9 Related Classifications
HY-P11916 Related Classifications
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