WSP-1
Based on 2 publication(s) in Google Scholar
WSP-1 is a selective and rapid-reaction H2S specific fluorescent dye (Ex/Em=465/515 nm). WSP-1 reacts with H2S with the releasing of fluorophore.
For research use only. We do not sell to patients.
- Purity: 99.20%
- CAS No.: 1352750-34-5
- Formula: C33H21NO6S2
- Molecular Weight:591.65
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) WSP-1
More
Customer Validation & Images
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IF
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Flow Cytometry
Biological Activity
WSP-1 have fluorescence properties, with excitation wavelength=465 nm, emission spectrum=515 nm[1].
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Fluorimetric Detection of Intracellular H2S (Fluorimetric Microscope Assessment)[1]:
1. Culture HASMCs up to about 90% confluence, and 24 h before the experiment, seed cells onto a 8-well culture slide at density of 30*103 per well and culture for 24 h.
2. Replace the culture medium, and incubate the cells for 30 min at 37°C in 400 μL per well WSP-1 working solution for 30 min.
3. Replace the WSP-1 working solution with 380 μL of buffer standard and add 20 μL of the tested H2S-donor compounds at the desired concentration.
4. When WSP-1 reacts with H2S, it releases a fluorophore detect able at Ex/Em=465/515 nm. Remove the buffer standard, and wash with 400 μL of DPBS after 1 h incubation.
5. Remove the DPBS, and add 200 μL of Bouin solution per well for 10 min at room temperature to fix the cells. Remove the excess of Bouin solution and wash the cells twice with 400 μL of DPBS.
6. Acquire the fluorescence signal with a fluorescence microscope.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1352750-34-5
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Appearance Solid
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Molecular Weight 591.65
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Formula C33H21NO6S2
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Color White to off-white
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SMILES
O=C(OC1=CC(OC2=C3C=CC(OC)=C2)=C(C43OC(C5=C4C=CC=C5)=O)C=C1)C6=CC=CC=C6SSC7=NC=CC=C7
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (2)
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Journal Impact Factor
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Most Recent
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ACS Nano
Overcoming Photothermal Resistance of Gastric Cancer by Bionic 2D Iron-Based Nanoplatforms with Precise CRISPR/Cas9 Delivery. [Abstract]2025 May 20;19(19):18188-18202. PMID: 40340328
WSP-1 purchased from MedChemExpress. Usage Cited in: ACS Nano. 2025 May 20;19(19):18188-18202. [Abstract]
H2S generation level of MGC 803 cells incubated with WSP-1 as a fluorescent probe.
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Sci Rep
Explore the role of CBS in stomach adenocarcinoma based on the sulfur-containing amino acid metabolism network. [Abstract]2025 Aug 6;15(1):28829. PMID: 40770005
WSP-1 purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 Aug 6;15(1):28829. [Abstract]
WSP-1 (15 µM). Although H2S exhibited a decreasing trend, no significant difference was observed, indicating that the 50% efficiency of CBS silencing was inadequate to impact H2S production.
Solvent & Solubility
DMSO : 25 mg/mL (42.25 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.6902 mL | 8.4509 mL | 16.9019 mL | 42.2547 mL |
| 5 mM | 0.3380 mL | 1.6902 mL | 3.3804 mL | 8.4509 mL | |
| 10 mM | 0.1690 mL | 0.8451 mL | 1.6902 mL | 4.2255 mL | |
| 15 mM | 0.1127 mL | 0.5634 mL | 1.1268 mL | 2.8170 mL | |
| 20 mM | 0.0845 mL | 0.4225 mL | 0.8451 mL | 2.1127 mL | |
| 25 mM | 0.0676 mL | 0.3380 mL | 0.6761 mL | 1.6902 mL | |
| 30 mM | 0.0563 mL | 0.2817 mL | 0.5634 mL | 1.4085 mL | |
| 40 mM | 0.0423 mL | 0.2113 mL | 0.4225 mL | 1.0564 mL |
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