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  3. WSP-1

WSP-1 is a selective and rapid-reaction H2S specific fluorescent dye (Ex/Em=465/515 nm). WSP-1 reacts with H2S with the releasing of fluorophore.

For research use only. We do not sell to patients.

WSP-1 Chemical Structure

WSP-1 Chemical Structure

CAS No. : 1352750-34-5

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1 mg USD 90 In-stock
5 mg USD 270 In-stock
10 mg USD 430 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

WSP-1 is a selective and rapid-reaction H2S specific fluorescent dye (Ex/Em=465/515 nm). WSP-1 reacts with H2S with the releasing of fluorophore[1].

In Vitro

WSP-1 have fluorescence properties, with excitation wavelength=465 nm, emission spectrum=515 nm[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Fluorimetric Detection of Intracellular H2S (Fluorimetric Microscope Assessment)[1]:
1. Culture HASMCs up to about 90% confluence, and 24 h before the experiment, seed cells onto a 8-well culture slide at density of 30*103 per well and culture for 24 hours.
2. Replace the culture medium, and incubate the cells for 30 min at 37°C in 400 μL per well WSP-1 working solution for 30 min.
3. Replace the WSP-1 working solution with 380 μL of buffer standard and add 20 μL of the tested H2S-donor compounds at the desired concentration.
4. When WSP-1 reacts with H2S, it releases a fluorophore detect able at Ex/Em=465/515 nm. Remove the buffer standard, and wash with 400 μL of DPBS after 1 h incubation.
5. Remove the DPBS, and add 200 μL of Bouin solution per well for 10 min at room temperature to fix the cells. Remove the excess of Bouin solution and wash the cells twice with 400 μL of DPBS.
6. Acquire the fluorescence signal with a fluorescence microscope.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

591.65

Formula

C33H21NO6S2

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(OC1=CC(OC2=C3C=CC(OC)=C2)=C(C43OC(C5=C4C=CC=C5)=O)C=C1)C6=CC=CC=C6SSC7=NC=CC=C7

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (42.25 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6902 mL 8.4509 mL 16.9019 mL
5 mM 0.3380 mL 1.6902 mL 3.3804 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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Volume (start)

V1

=
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C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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mg/kg

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g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6902 mL 8.4509 mL 16.9019 mL 42.2547 mL
5 mM 0.3380 mL 1.6902 mL 3.3804 mL 8.4509 mL
10 mM 0.1690 mL 0.8451 mL 1.6902 mL 4.2255 mL
15 mM 0.1127 mL 0.5634 mL 1.1268 mL 2.8170 mL
20 mM 0.0845 mL 0.4225 mL 0.8451 mL 2.1127 mL
25 mM 0.0676 mL 0.3380 mL 0.6761 mL 1.6902 mL
30 mM 0.0563 mL 0.2817 mL 0.5634 mL 1.4085 mL
40 mM 0.0423 mL 0.2113 mL 0.4225 mL 1.0564 mL
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WSP-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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WSP-1
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