β-S-ARCA triammonium
Based on 1 Customer Validation
β-S-ARCA (3'-O-Me-m7G(5')ppp(5')G) triammonium is a mRNA 7-methylguanosine (m7G) cap analog carrying a phosphorothioate (PS) moiety. β-S-ARCA triammonium binds eIF4E via electrostatic interactions between its β-sulfur atom and positively charged Arg and Lys residues in the protein binding site. β-S-ARCA triammonium prevents the decapping by Dcp2, increases the mRNA half-life, enhances cap-dependent translation, and increases protein expression in cells. β-S-ARCA triammonium has been applied in researching experimental mRNA-based anticancer vaccines.
For research use only. We do not sell to patients.
- Purity: 99.48%
- Formula: C22H40N13O17P3S
- Molecular Weight:883.62
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All DNA/RNA Synthesis Isoforms
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Biological Activity
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eIF4E |
β-S-ARCA triammonium exists as a pair of diastereomer D1 and D2 with D1 binding purified eIF4E with a Kd of 23.2 nM, and D2 binding purified eIF4E with a Kd of 51.8 nM,[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 883.62
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Formula C22H40N13O17P3S
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Color White to off-white
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SMILES
O=P(O)(OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C=NC3=C2NC(N)=NC3=O)O1)OP(OP(OC[C@@H]4[C@@H](O)[C@@H](OC)[C@H](N5C(NC(N)=N6)=C([N+](C)=C5)C6=O)O4)([O-])=O)(S)=O.N.N.N
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Synonyms
3’-O-Me-m7G(5')ppp(5')G triammonium
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : 50 mg/mL (56.59 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (282 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Warminski M, et al. Chemical Modifications of mRNA Ends for Therapeutic Applications. Acc Chem Res. 2023;56(20):2814-2826. [Content Brief]
[2]. Wojtczak BA, et al. 5'-Phosphorothiolate Dinucleotide Cap Analogues: Reagents for Messenger RNA Modification and Potent Small-Molecular Inhibitors of Decapping Enzymes. J Am Chem Soc. 2018;140(18):5987-5999. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.1317 mL | 5.6585 mL | 11.3171 mL | 28.2927 mL |
| 5 mM | 0.2263 mL | 1.1317 mL | 2.2634 mL | 5.6585 mL | |
| 10 mM | 0.1132 mL | 0.5659 mL | 1.1317 mL | 2.8293 mL | |
| 15 mM | 0.0754 mL | 0.3772 mL | 0.7545 mL | 1.8862 mL | |
| 20 mM | 0.0566 mL | 0.2829 mL | 0.5659 mL | 1.4146 mL | |
| 25 mM | 0.0453 mL | 0.2263 mL | 0.4527 mL | 1.1317 mL | |
| 30 mM | 0.0377 mL | 0.1886 mL | 0.3772 mL | 0.9431 mL | |
| 40 mM | 0.0283 mL | 0.1415 mL | 0.2829 mL | 0.7073 mL | |
| 50 mM | 0.0226 mL | 0.1132 mL | 0.2263 mL | 0.5659 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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