2',4'-Dihydroxydihydrochalcone
2',4'-Dihydroxydihydrochalcone exhibits cytotoxic activity against HT-29 colon adenocarcinoma cells (IC50 = 1.8 μM). 2',4'-Dihydroxydihydrochalcone may be used in research on cancers such as colon adenocarcinoma.
For research use only. We do not sell to patients.
- CAS No.: 53596-71-7
- Formula: C15H14O3
- Molecular Weight:242.27
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | IC50 |
249 μM
Compound: 7
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Cytotoxicity against human HEK293 cells assessed as cell viability after 24 hrs by cell titer-blue assay
Cytotoxicity against human HEK293 cells assessed as cell viability after 24 hrs by cell titer-blue assay
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[PMID: 26473275] |
Chemical Information
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CAS No. 53596-71-7
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Molecular Weight 242.27
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Formula C15H14O3
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SMILES
O=C(CCC1=CC=CC=C1)C2=CC=C(C=C2O)O
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
2',4'-Dihydroxydihydrochalcone Related Classifications
HY-N17610 Related Classifications
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