2. Cell Cycle/DNA Damage
  3. DNA/RNA Synthesis
  4. 2'-O-MOE-U

2'-O-MOE-U is a nucleic acid modification group (Phosphoramidite) with 3'-exonuclease inhibitory activity. 2'-O-MOE-U also exhibits gene silencing activity and double-stranded oligonucleotide stability. By forming steric interactions with 3'-exonuclease residues, 2'-O-MOE-U anchors the 3'-end of the siRNA guide strand in the hAgo2 PAZ domain, thereby regulating double-stranded thermal stability and enhancing base-pairing specificity. 2'-O-MOE-U does not induce IFNα production, can be incorporated at multiple sites of siRNA to enhance RNAi activity, and produces a synergistic effect with 2'-F modification. 2'-O-MOE-U has been widely used in studies related to breast cancer and other diseases.

For research use only. We do not sell to patients.

2'-O-MOE-U

2'-O-MOE-U Chemical Structure

CAS No. : 163759-97-5

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2'-O-MOE-U Related Antibodies

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Description

2'-O-MOE-U is a nucleic acid modification group (Phosphoramidite) with 3'-exonuclease inhibitory activity. 2'-O-MOE-U also exhibits gene silencing activity and double-stranded oligonucleotide stability. By forming steric interactions with 3'-exonuclease residues, 2'-O-MOE-U anchors the 3'-end of the siRNA guide strand in the hAgo2 PAZ domain, thereby regulating double-stranded thermal stability and enhancing base-pairing specificity. 2'-O-MOE-U does not induce IFNα production, can be incorporated at multiple sites of siRNA to enhance RNAi activity, and produces a synergistic effect with 2'-F modification. 2'-O-MOE-U has been widely used in studies related to breast cancer and other diseases[1][2].

In Vitro

2′-O-MOE-U (35 mU/mL SVPD; up to 180 min) modification increases the stability of dT10-mer oligonucleotides against 3′-exonuclease (SVPD), with ON7 exhibiting a T1/2 of ~22 min[1].
2'-O-MOE-U modification increases the serum stability of 14-mer single-stranded DNA, with a half-life of 206.9 min in 10% FBS, compared to 33.4 min for unmodified DNA[2].
2'-O-MOE-U (0.5 nM) reduces off-target effects while exhibiting approximately 60% targeted gene silencing at 0.5 nM in lipofected cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2'-O-MOE-U Related Antibodies
Molecular Weight

804.86

Formula

C42H53N4O10P
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

COC(C=C1)=CC=C1C(C2=CC=C(OC)C=C2)(C3=CC=CC=C3)OC[C@H]4O[C@@H](N5C(NC(C=C5)=O)=O)[C@H](OCCOC)[C@@H]4OP(N(C(C)C)C(C)C)OCCC#N
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 50 mg/mL (62.12 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2425 mL 6.2123 mL 12.4245 mL
5 mM 0.2485 mL 1.2425 mL 2.4849 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation

Purity: ≥95.0%

SDS (392 KB)

COA (260 KB) HNMR (128 KB) CNMR (131 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.2425 mL 6.2123 mL 12.4245 mL 31.0613 mL
5 mM 0.2485 mL 1.2425 mL 2.4849 mL 6.2123 mL
10 mM 0.1242 mL 0.6212 mL 1.2425 mL 3.1061 mL
15 mM 0.0828 mL 0.4142 mL 0.8283 mL 2.0708 mL
20 mM 0.0621 mL 0.3106 mL 0.6212 mL 1.5531 mL
25 mM 0.0497 mL 0.2485 mL 0.4970 mL 1.2425 mL
30 mM 0.0414 mL 0.2071 mL 0.4142 mL 1.0354 mL
40 mM 0.0311 mL 0.1553 mL 0.3106 mL 0.7765 mL
50 mM 0.0248 mL 0.1242 mL 0.2485 mL 0.6212 mL
60 mM 0.0207 mL 0.1035 mL 0.2071 mL 0.5177 mL
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2'-O-MOE-U Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2'-O-MOE-U163759-97-5DNA/RNA Synthesisbreast cancerDNA:RNA hybrid duplexsiRNA guide strand5'-exonucleasesingle-stranded DNAgene silencing3'-exonucleasehAgo2 PAZ domainoligonucleotide duplexRNAi activityInhibitorinhibitorinhibit

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