(2R,3R)-Butane-2,3-diol
Based on 1 Customer Validation
(2R,3R)-Butane-2,3-diol is a non-covalent, reversible agonist targeting lanthanum (La3+)-sensitive calcium channels in bacteria (e.g., Escherichia coli) with an EC50 of approximately 25 mM. (2R,3R)-Butane-2,3-diol binds to calcium channel proteins or related complexes, induces channel opening, promotes extracellular calcium influx, and triggers intracellular calcium transients, which may regulate bacterial physiological activities such as growth, metabolism, and signal transduction. (2R,3R)-Butane-2,3-diol mediates bacterial-host cell signaling interactions and affects the metabolic balance of intestinal microorganisms, and can be used to study lactose intolerance and other related diseases.
For research use only. We do not sell to patients.
- Purity: 99.69%
- CAS No.: 24347-58-8
- Formula: C4H10O2
- Molecular Weight:90.12
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Storage:Pure form -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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La3+-sensitive calcium channel 25 mM (EC50) |
(2R,3R)-Butane-2,3-diol (100 mM; 1-2 min) significantly induces a rapid increase in intracellular free Ca2+ concentration in the Escherichia coli intracellular calcium transient assay, with the peak value increasing by about 1-2 μM compared with the control group, and this effect is dependent on the external Ca2+ concentration (0.1-10 mM)[1].
(2R,3R)-Butane-2,3-diol (5-200 mM; 5 min) activates the E. coli Ca2+ channel in a dose-dependent manner in the concentration-dependent assay, with EC50 of about 25 mM. Under the condition of 10 mM external Ca2+, the peak intracellular Ca2+ could reach 25 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 24347-58-8
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Appearance Liquid (Density: 0.992 g/cm3)
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Molecular Weight 90.12
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Formula C4H10O2
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Color Colorless to light yellow
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SMILES
C[C@@H](O)[C@H](O)C
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (1109.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (27.74 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.72 mg/mL (19.09 mM); Clear solution
This protocol yields a clear solution of ≥ 1.72 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (17.2 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 11.0963 mL | 55.4816 mL | 110.9632 mL | 277.4079 mL |
| 5 mM | 2.2193 mL | 11.0963 mL | 22.1926 mL | 55.4816 mL | |
| 10 mM | 1.1096 mL | 5.5482 mL | 11.0963 mL | 27.7408 mL | |
| 15 mM | 0.7398 mL | 3.6988 mL | 7.3975 mL | 18.4939 mL | |
| 20 mM | 0.5548 mL | 2.7741 mL | 5.5482 mL | 13.8704 mL | |
| 25 mM | 0.4439 mL | 2.2193 mL | 4.4385 mL | 11.0963 mL | |
| 30 mM | 0.3699 mL | 1.8494 mL | 3.6988 mL | 9.2469 mL | |
| 40 mM | 0.2774 mL | 1.3870 mL | 2.7741 mL | 6.9352 mL | |
| 50 mM | 0.2219 mL | 1.1096 mL | 2.2193 mL | 5.5482 mL | |
| 60 mM | 0.1849 mL | 0.9247 mL | 1.8494 mL | 4.6235 mL | |
| 80 mM | 0.1387 mL | 0.6935 mL | 1.3870 mL | 3.4676 mL | |
| 100 mM | 0.1110 mL | 0.5548 mL | 1.1096 mL | 2.7741 mL |
(2R,3R)-Butane-2,3-diol Related Classifications
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