(2R)-Kazinol B
(2R)-Kazinol B is a prenylated flavane enantiomer found in the stem and root barks of Daphne giraldii, and it is also an isomer of Kazinol B (HY-N3426). (2R)-Kazinol B exerts selective cytotoxicity against liver cancer cells, induces apoptosis via both intrinsic and extrinsic pathways, and triggers protective autophagy through AMPK activation. (2R)-Kazinol B can be used in the research of liver cancer.
For research use only. We do not sell to patients.
- CAS No.: 2097138-89-9
- Formula: C25H28O4
- Molecular Weight:392.49
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Biological Activity
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AMPK |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Hep3B | IC50 |
30.3 μM
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Antiproliferative activity against human Hep3B hepatic carcinoma cells assessed as cell growth inhibition incubated for 48 hrs by MTT assay.
Antiproliferative activity against human Hep3B hepatic carcinoma cells assessed as cell growth inhibition incubated for 48 hrs by MTT assay.
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28371676 |
(2R)-Kazinol B (compound (±)-4) (6.25-100 μM; 48 h) potently inhibits the proliferation of human hepatocellular carcinoma Hep3B cells, with an IC50 value of 30.3 μM[1].
(2R)-Kazinol B (15-60 μM) activates AMPK in human hepatocellular carcinoma Hep3B cells, and this activation is positively correlated with the protective autophagy induced by (2R)-Kazinol B[1].
(2R)-Kazinol B (15-60 μM; 48 h) induces apoptosis and autophagy in human hepatocellular carcinoma Hep3B cells via intrinsic and extrinsic pathways[1].
(2R)-Kazinol B (30 μM)‑induced autophagy exerts a protective effect against apoptotic death in human hepatocellular carcinoma Hep3B cells, and inhibition of autophagy enhances (2R)-kazinol B‑mediated apoptosis[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Hep3B human hepatic carcinoma cells
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Concentration:6.25, 12.5, 25, 50, 100 μM
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Incubation Time:48 h
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Result:Exhibited selective cytotoxicity toward hepatic carcinoma cells, with potent inhibitory activity against Hep3B cells.
Reached an IC50 value of 30.3 μM against Hep3B cells.
Showed two to three-fold stronger cytotoxicity than its racemic mixture, compound 4.
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Cell Line:Hep3B human hepatic carcinoma cells
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Concentration:30 μM
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Incubation Time:48 h
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Result:Caused cellular shrinkage, membrane blebbing, chromatin condensation, and nuclear fragmentation in Hep3B cells.
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Cell Line:Hep3B human hepatic carcinoma cells
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Concentration:15, 30, 60 μM
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Incubation Time:48 h
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Result:Increased protein levels of FADD, cleaved caspase 8, Bax, cleaved caspase 9, cleaved caspase 7, and cleaved PARP in a concentration-dependent manner.
Decreased full-length PARP protein levels in a concentration-dependent manner.
Decreased p62 protein levels in a concentration-dependent manner.
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Cell Line:Hep3B human hepatic carcinoma cells
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Concentration:30 μM
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Incubation Time:48 h
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Result:Caused a marked increase in MDC-positive cells, indicating accumulation of autophagic vacuoles.
Increased the conversion of LC3 I to LC3 II in a concentration-dependent manner.
Chemical Information
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CAS No. 2097138-89-9
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Molecular Weight 392.49
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Formula C25H28O4
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SMILES
C/C(C)=C\CC1=C([C@@H]2OC3=CC(O)=CC=C3CC2)C=C4C(OC(C)(C=C4)C)=C1O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
(2R)-Kazinol B Related Classifications
HY-N3426A Related Classifications
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