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3-CPs (Synonyms: 3-Carbethoxypsoralen; 3-Ethoxycarbonylpsoralen)

Cat. No.: HY-U00265
Handling Instructions

3-CPs is a serotype capsular polysaccharide which can interfere with antibody-mediated bacterial killing.

For research use only. We do not sell to patients.

3-CPs Chemical Structure

3-CPs Chemical Structure

CAS No. : 20073-24-9

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Description

3-CPs is a serotype capsular polysaccharide which can interfere with antibody-mediated bacterial killing.

In Vitro

3-CPs displays protective effect against UVB damage in all concentrations tested[1]. About 50% inhibition of bacterial killing is observed when 12 ng of purified type 3 CPS is added to the OPKA. The addition of 30 μL of mouse serum post-i.p. infection with WU2 (containing 0.4 to 0.7 μg/mL released type 3 CPS) shows 26% to 52% inhibition of bacterial killing, comparable to what is observed when an equivalent amount of purified CPS is added[2].

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
Kinase Assay
[2]

To measure the inhibitory effect of releaseing type 3-CPs on bacteria killing, an OPKA is performed with the addition of culture supernatant or mouse serum obtained at 24 h following i.p. injection with 103 CFU of the S. pneumoniae WU2 strain. In each well, 10 μL of diluted rabbit serum is mixed with 10 μL of strain WU2 (1,000 CFU) and 10 μL of culture supernatant or mouse serum for 30 min at room temperature before HL-60 cells and complement are added. The percent inhibition of bacterial killing is compared to that with the OPKA with the addition of ΔCPS WU2 culture supernatant or naive mouse serum containing no 3-CPs[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

258.23

Formula

C₁₄H₁₀O₅

CAS No.

20073-24-9

SMILES

O=C(C1=CC2=CC3=C(OC=C3)C=C2OC1=O)OCC

Shipping

Room temperature in continental US; may vary elsewhere

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3-CPs
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3-CPs

Cat. No.: HY-U00265