2. Metabolic Enzyme/Protease Neuronal Signaling GPCR/G Protein Immunology/Inflammation NF-κB
  3. Endogenous Metabolite Adrenergic Receptor Reactive Oxygen Species (ROS)
  4. 3-Nitro-L-tyrosine

3-Nitro-L-tyrosine serves as a biomarker of oxidative stress. 3-Nitro-L-tyrosine attenuates the pressor and vasoconstrictive effects of angiotensin II by inhibiting the α1-adrenergic receptor-mediated signaling pathway, and participates in hemodynamic regulation under pathological conditions such as inflammation and ischemia. 3-Nitro-L-tyrosine can be used in studies related to atherosclerosis, ischemia-reperfusion and sepsis.

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3-Nitro-L-tyrosine

3-Nitro-L-tyrosine Chemical Structure

CAS No. : 621-44-3

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Based on 1 publication(s) in Google Scholar

Other Forms of 3-Nitro-L-tyrosine:

3-Nitro-L-tyrosine Related Antibodies

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Description

3-Nitro-L-tyrosine serves as a biomarker of oxidative stress. 3-Nitro-L-tyrosine attenuates the pressor and vasoconstrictive effects of angiotensin II by inhibiting the α1-adrenergic receptor-mediated signaling pathway, and participates in hemodynamic regulation under pathological conditions such as inflammation and ischemia. 3-Nitro-L-tyrosine can be used in studies related to atherosclerosis, ischemia-reperfusion and sepsis[1][2].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

3-Nitro-L-tyrosine can be found in the brain as a product of superoxide and nitric oxide, and serves as a marker for oxidative stress induced by reactive oxygen species and nitric oxide[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

3-Nitro-L-tyrosine Related Antibodies
In Vivo

3-Nitro-L-tyrosine (2.5 μmol/kg; intravenous injection; single bolus administration) attenuates angiotensin II-induced pressor responses and renal/mesenteric vasoconstrictor responses in pentobarbital-anesthetized rats[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Sprague-Dawley (male, 250-350 g, pentobarbital-anesthetized, surgically implanted with hemodynamic monitoring catheters and Doppler flow probes)[2]
Dosage: 2.5 μmol/kg
Administration: i.v.; single bolus
Result: Produced a significant 36% increase in baseline hindquarter vascular resistance at 120-180 min post-treatment.
Diminished the dose-dependent pressor responses to angiotensin II at 120-180 min post-treatment.
Significantly reduced the dose-dependent vasoconstrictor effects of angiotensin II in renal and mesenteric beds at 30-60 min post-treatment, with further attenuation evident at 120-180 min post-treatment.
Did not alter the minor effects of angiotensin II on hindquarter vascular resistance.
Molecular Weight

226.19

Formula

C9H10N2O5
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

N[C@@H](CC1=CC([N+]([O-])=O)=C(C=C1)O)C(O)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 4.76 mg/mL (21.04 mM; ultrasonic and adjust pH to 2 with 1 M HCl)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.4211 mL 22.1053 mL 44.2106 mL
5 mM 0.8842 mL 4.4211 mL 8.8421 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.89%

SDS (393 KB)

COA (247 KB) HNMR (249 KB) RP-HPLC (255 KB) NP-HPLC (164 KB) MS (68 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 4.4211 mL 22.1053 mL 44.2106 mL 110.5265 mL
5 mM 0.8842 mL 4.4211 mL 8.8421 mL 22.1053 mL
10 mM 0.4421 mL 2.2105 mL 4.4211 mL 11.0527 mL
15 mM 0.2947 mL 1.4737 mL 2.9474 mL 7.3684 mL
20 mM 0.2211 mL 1.1053 mL 2.2105 mL 5.5263 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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3-Nitro-L-tyrosine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3-Nitro-L-tyrosine621-44-3Endogenous MetaboliteAdrenergic ReceptorReactive Oxygen Species (ROS)Beta Receptorhuman cerebrum gray mattersepsisischemia-reperfusionpentobarbital-anesthetized ratsatherosclerosisoxidative stressadrenoceptorangiotensin IIInhibitorinhibitorinhibit

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