4-Azidophenol
Based on 1 Customer Validation
4-Azidophenol (p-Azidophenol) is a click chemistry reagent containing an azide group that can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. 4-Azidophenol can also react with molecules containing DBCO or BCN groups through strain-promoted azide-alkyne cycloaddition (SPAAC).
For research use only. We do not sell to patients.
- Purity : 99.83%
- CAS No.: 24541-43-3
- Formula: C6H5N3O
- Molecular Weight:135.12
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Storage:Pure form -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Chemical Information
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CAS No. 24541-43-3
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Appearance Oil
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Molecular Weight 135.12
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Formula C6H5N3O
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Color Brown to black
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SMILES
C1=C(C=CC(=C1)O)N=[N+]=[N-]
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Synonyms
p-Azidophenol
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 5 mg/mL (37.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
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Data Sheet (265 KB)
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SDS (643 KB)
- English - EN (643 KB)
- Français - FR (643 KB)
- Deutsch - DE (643 KB)
- Norwegian - NO (643 KB)
- Español - ES (643 KB)
- Swedish - SV (643 KB)
- Italian - IT (643 KB)
- Korean - KR (643 KB)
- Portuguese - PT (643 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 7.4008 mL | 37.0041 mL | 74.0083 mL | 185.0207 mL |
| 5 mM | 1.4802 mL | 7.4008 mL | 14.8017 mL | 37.0041 mL | |
| 10 mM | 0.7401 mL | 3.7004 mL | 7.4008 mL | 18.5021 mL | |
| 15 mM | 0.4934 mL | 2.4669 mL | 4.9339 mL | 12.3347 mL | |
| 20 mM | 0.3700 mL | 1.8502 mL | 3.7004 mL | 9.2510 mL | |
| 25 mM | 0.2960 mL | 1.4802 mL | 2.9603 mL | 7.4008 mL | |
| 30 mM | 0.2467 mL | 1.2335 mL | 2.4669 mL | 6.1674 mL |
4-Azidophenol Related Classifications
HY-W178441 Related Classifications
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