4-Bromobenzoic acid-d4
Based on 1 Customer Validation
4-Bromobenzoic acid-d4 (Benzoic-2,3,5,6-d4 acid, 4-bromo- (9CI) is the d4-labeled 4-Bromobenzoic acid (HY-78700). 4-Bromobenzoic acid is a Dehalogenase substrate and intermediate. 4-Bromobenzoic acid undergoes hydrolytic dehalogenation catalyzed by the dehalogenase of Cupriavidus sp. strain SK-3 to produce 4-Hydroxybenzoic acid (HY-Y0264). 4-Bromobenzoic acid can be utilized as the sole carbon and energy source. 4-Bromobenzoic acid can be used for the synthesis of α-amylase inhibitors.
For research use only. We do not sell to patients.
- Purity : 98.09%
- CAS No.: 787624-24-2
- Formula: C7HD4BrO2
- Molecular Weight:205.04
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Application
1. This compound can be used as a tracer
2. This compound can be used as an internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.
Chemical Information
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CAS No. 787624-24-2
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Unlabeled CAS 586-76-5
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Appearance Solid
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Molecular Weight 205.04
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Formula C7HD4BrO2
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Color White to off-white
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SMILES
O=C(O)C1=C([2H])C([2H])=C(Br)C([2H])=C1[2H]
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Synonyms
Benzoic-2,3,5,6-d4 acid, 4-bromo- (9CI
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 125 mg/mL (609.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (10.14 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (10.14 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 4.8771 mL | 24.3855 mL | 48.7710 mL | 121.9274 mL |
| 5 mM | 0.9754 mL | 4.8771 mL | 9.7542 mL | 24.3855 mL | |
| 10 mM | 0.4877 mL | 2.4385 mL | 4.8771 mL | 12.1927 mL | |
| 15 mM | 0.3251 mL | 1.6257 mL | 3.2514 mL | 8.1285 mL | |
| 20 mM | 0.2439 mL | 1.2193 mL | 2.4385 mL | 6.0964 mL | |
| 25 mM | 0.1951 mL | 0.9754 mL | 1.9508 mL | 4.8771 mL | |
| 30 mM | 0.1626 mL | 0.8128 mL | 1.6257 mL | 4.0642 mL | |
| 40 mM | 0.1219 mL | 0.6096 mL | 1.2193 mL | 3.0482 mL | |
| 50 mM | 0.0975 mL | 0.4877 mL | 0.9754 mL | 2.4385 mL | |
| 60 mM | 0.0813 mL | 0.4064 mL | 0.8128 mL | 2.0321 mL | |
| 80 mM | 0.0610 mL | 0.3048 mL | 0.6096 mL | 1.5241 mL | |
| 100 mM | 0.0488 mL | 0.2439 mL | 0.4877 mL | 1.2193 mL |
4-Bromobenzoic acid-d4 Related Classifications
HY-W249257 Related Classifications
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Keywords
- 4-Bromobenzoic acid-d4
- 787624-24-2
- Benzoic-2,3,5,6-d4 acid, 4-bromo- (9CI
- Isotope-Labeled Compounds
- Bacterial
- Drug Intermediate
- Drug Iintermediate
- 4-hydroxybenzoic acid
- Cupriavidus sp. strain SK-3
- catabolized
- dehalogenase
- dehalogenase substrate
- growth substrate
- hydrolytic dehalogenation
- sole carbon and energy source
- whole-cell cultures
- Inhibitor
- inhibitor
- inhibit