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  3. 4-Hydroxybenzoic acid

4-Hydroxybenzoic acid, a phenolic derivative of benzoic acid, could inhibit most gram-positive and some gram-negative bacteria, with an IC50 of 160 μg/mL.

For research use only. We do not sell to patients.

4-Hydroxybenzoic acid Chemical Structure

4-Hydroxybenzoic acid Chemical Structure

CAS No. : 99-96-7

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10 mM * 1 mL in DMSO
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 4-Hydroxybenzoic acid:

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

4-Hydroxybenzoic acid, a phenolic derivative of benzoic acid, could inhibit most gram-positive and some gram-negative bacteria, with an IC50 of 160 μg/mL.

IC50 & Target[1]

Microbial Metabolite

 

Bacteria

160 μg/mL (IC50)

Human Endogenous Metabolite

 

In Vitro

Most of the gram-positive and some gram-negative bacteria are sensitive to trans 4-Hydroxycinnamic acid (4-HBA) and 4-Hydroxybenzoic acid at IC50 concentrations of 100-170 and 160 μg/mL, respectively. The antimicrobial activities of 4-Hydroxycinnamic acid and t4-HCA against 11 food pathogenic bacteria, 6 plant pathogenic bacteria, 2 yeasts and 15 plant pathogenic fungi are tested by the paper disc method. These compounds inhibit the growth of most of the bacteria and yeasts at concentrations of 200-400 μg. However, the inhibition is more effective against most of the gram-positive bacteria. When tested by the paper disc method, 4-Hydroxycinnamic acid has stronger antimicrobial activity than t4-HCA against S. aureus, L. mesenteroides, S. cerevisiae and C. albicans at a concentration of 50 μg. However, no inhibitory effect against fungi was observed at concentrations even up to 1000 μg[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

138.12

Formula

C7H6O3

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(O)C1=CC=C(O)C=C1

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 120 mg/mL (868.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 7.2401 mL 36.2004 mL 72.4008 mL
5 mM 1.4480 mL 7.2401 mL 14.4802 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 3 mg/mL (21.72 mM); Clear solution

    This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 3 mg/mL (21.72 mM); Clear solution

    This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.93%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 7.2401 mL 36.2004 mL 72.4008 mL 181.0020 mL
5 mM 1.4480 mL 7.2401 mL 14.4802 mL 36.2004 mL
10 mM 0.7240 mL 3.6200 mL 7.2401 mL 18.1002 mL
15 mM 0.4827 mL 2.4134 mL 4.8267 mL 12.0668 mL
20 mM 0.3620 mL 1.8100 mL 3.6200 mL 9.0501 mL
25 mM 0.2896 mL 1.4480 mL 2.8960 mL 7.2401 mL
30 mM 0.2413 mL 1.2067 mL 2.4134 mL 6.0334 mL
40 mM 0.1810 mL 0.9050 mL 1.8100 mL 4.5251 mL
50 mM 0.1448 mL 0.7240 mL 1.4480 mL 3.6200 mL
60 mM 0.1207 mL 0.6033 mL 1.2067 mL 3.0167 mL
80 mM 0.0905 mL 0.4525 mL 0.9050 mL 2.2625 mL
100 mM 0.0724 mL 0.3620 mL 0.7240 mL 1.8100 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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4-Hydroxybenzoic acid
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