5-Acetylsalicylic acid
Based on 1 publication(s) in Google Scholar
5-Acetylsalicylic acid is the derivate of Aspirin (HY-14654), with anti-inflammatory property. 5-Acetylsalicylic acid is the active metabolite of Sulfasalazine (HY-14655) and its production is regulated by beneficial bacteria in the gut but is confined to the intestinal tract. 5-Acetylsalicylic acid exhibits a mild cytotoxicity effect on B16F10 cells. 5-Acetylsalicylic acid can be used for the study of inflammatory bowel disease (IBD) such as ulcerative colitis (UC).
For research use only. We do not sell to patients.
- Purity: 99.84%
- CAS No.: 13110-96-8
- Formula: C9H8O4
- Molecular Weight:180.16
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Bioactive Compound Library Max
HY-L181
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Bioactive Compound Library
HY-L001
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Immunology/Inflammation Compound Library
HY-L007
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FDA-Approved Drug Library
HY-L022
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Anti-Cancer Compound Library
HY-L025
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HY-L035
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HY-L049
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FDA Approved & Pharmacopeial Drug Library
HY-L066
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EMA-Approved Drug Library
HY-L116
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FDA-Approved Anticancer Drug Library
HY-L122
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Non-steroidal Anti-Inflammatory Compound Library
HY-L130
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HY-L141
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HY-L001V
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Drug Repurposing Compound Library Plus
HY-L035P
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FDA-Approved Drug Library Plus
HY-L022P
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FDA-Approved Drug Library Mini
HY-L022M
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High-Throughput Bioactive Compound Library
HY-L205
Publications Citing Use of MedChemExpress (MCE) 5-Acetylsalicylic acid
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Biological Activity
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Cell Line
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Type | Value | Description | References |
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| B16-F10 | IC50 |
4879 μM
Compound: 5AcSH
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Cytotoxicity against mouse B16F10 cells assessed as cell viability after 72 hrs by MTT assay
Cytotoxicity against mouse B16F10 cells assessed as cell viability after 72 hrs by MTT assay
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[PMID: 26774931] |
| L929 | IC50 |
305.6 μM
Compound: 5AcSH
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Cytotoxicity against mouse L929 cells assessed as cell viability after 72 hrs by MTT assay
Cytotoxicity against mouse L929 cells assessed as cell viability after 72 hrs by MTT assay
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[PMID: 26774931] |
5-Acetylsalicylic acid inhibits B16F10 and murine L929 fibroblasts cell proliferation with IC50s of 4879 μM and 305.6 μM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 13110-96-8
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Appearance Solid
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Molecular Weight 180.16
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Formula C9H8O4
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Color Off-white to light yellow
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SMILES
O=C(O)C1=CC(C(C)=O)=CC=C1O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (1)
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Journal Impact Factor
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Most Recent
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Cancers (Basel)
Drug Repurposing to Identify a Synergistic High-Order Drug Combination to Treat Sunitinib-Resistant Renal Cell Carcinoma. [Abstract]2021 Aug 6;13(16):3978. PMID: 34439134
Solvent & Solubility
DMSO : 100 mg/mL (555.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.88 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.88 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
References
[1]. Lee HJ, et al. The influence of probiotic treatment on sulfasalazine metabolism in rat. Xenobiotica. 2012 Aug;42(8):791-7. [Content Brief]
[2]. Islam A, et al. Cytotoxicity and apoptotic activity of novel organobismuth(V) and organoantimony(V) complexes in different cancer cell lines. Eur J Med Chem. 2016 Feb 15;109:254-67. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 5.5506 mL | 27.7531 mL | 55.5062 mL | 138.7655 mL |
| 5 mM | 1.1101 mL | 5.5506 mL | 11.1012 mL | 27.7531 mL | |
| 10 mM | 0.5551 mL | 2.7753 mL | 5.5506 mL | 13.8766 mL | |
| 15 mM | 0.3700 mL | 1.8502 mL | 3.7004 mL | 9.2510 mL | |
| 20 mM | 0.2775 mL | 1.3877 mL | 2.7753 mL | 6.9383 mL | |
| 25 mM | 0.2220 mL | 1.1101 mL | 2.2202 mL | 5.5506 mL | |
| 30 mM | 0.1850 mL | 0.9251 mL | 1.8502 mL | 4.6255 mL | |
| 40 mM | 0.1388 mL | 0.6938 mL | 1.3877 mL | 3.4691 mL | |
| 50 mM | 0.1110 mL | 0.5551 mL | 1.1101 mL | 2.7753 mL | |
| 60 mM | 0.0925 mL | 0.4626 mL | 0.9251 mL | 2.3128 mL | |
| 80 mM | 0.0694 mL | 0.3469 mL | 0.6938 mL | 1.7346 mL | |
| 100 mM | 0.0555 mL | 0.2775 mL | 0.5551 mL | 1.3877 mL |
5-Acetylsalicylic acid Related Classifications
HY-107831 Related Classifications
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