7-TFA-ap-7-Deaza-dA
Based on 1 Customer Validation
7-TFA-ap-7-Deaza-dA is a modified nucleoside. 7-TFA-ap-7-Deaza-dA can be used in the synthesis of deoxyribonucleic acid or nucleic acid. 7-TFA-ap-7-Deaza-dA is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
For research use only. We do not sell to patients.
- Purity : 98.81%
- CAS No.: 178420-75-2
- Formula: C16H16F3N5O4
- Molecular Weight:399.32
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
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Help & FAQs
All DNA/RNA Synthesis Isoforms
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Biological Activity
Description
Chemical Information
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CAS No. 178420-75-2
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Appearance Solid
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Molecular Weight 399.32
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Formula C16H16F3N5O4
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Color Off-white to brown
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SMILES
OC[C@@H]1[C@@H](O)C[C@H](N2C(N=CN=C3N)=C3C(C#CCNC(C(F)(F)F)=O)=C2)O1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
In Vitro:
DMSO : 250 mg/mL (626.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
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Data Sheet (246 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5043 mL | 12.5213 mL | 25.0426 mL | 62.6064 mL |
| 5 mM | 0.5009 mL | 2.5043 mL | 5.0085 mL | 12.5213 mL | |
| 10 mM | 0.2504 mL | 1.2521 mL | 2.5043 mL | 6.2606 mL | |
| 15 mM | 0.1670 mL | 0.8348 mL | 1.6695 mL | 4.1738 mL | |
| 20 mM | 0.1252 mL | 0.6261 mL | 1.2521 mL | 3.1303 mL | |
| 25 mM | 0.1002 mL | 0.5009 mL | 1.0017 mL | 2.5043 mL | |
| 30 mM | 0.0835 mL | 0.4174 mL | 0.8348 mL | 2.0869 mL | |
| 40 mM | 0.0626 mL | 0.3130 mL | 0.6261 mL | 1.5652 mL | |
| 50 mM | 0.0501 mL | 0.2504 mL | 0.5009 mL | 1.2521 mL | |
| 60 mM | 0.0417 mL | 0.2087 mL | 0.4174 mL | 1.0434 mL | |
| 80 mM | 0.0313 mL | 0.1565 mL | 0.3130 mL | 0.7826 mL | |
| 100 mM | 0.0250 mL | 0.1252 mL | 0.2504 mL | 0.6261 mL |
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