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CY3-YNE  (Synonyms: Sulfo-Cyanine3-alkyne)

Cat. No.: HY-D0818 Purity: 98.07%
Handling Instructions Technical Support

CY3-YNE (Sulfo-Cyanine3-alkyne) is a dye for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. CY3-YNE is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

For research use only. We do not sell to patients.

CY3-YNE

CY3-YNE Chemical Structure

CAS No. : 1010386-62-5

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Based on 1 publication(s) in Google Scholar

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Description

CY3-YNE (Sulfo-Cyanine3-alkyne) is a dye for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. CY3-YNE is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

Molecular Weight

668.84

Formula

C34H42N3O7S2

CAS No.
Appearance

Solid

Color

Brown to reddish brown

Emission (Em)

570

Excitation (Ex)

550

SMILES

CC(/C(N1CCCCCC(NCC#C)=O)=C\C=C\C2=[N+](CC)C(C=CC(S(=O)(O)=O)=C3)=C3C2(C)C)(C)C4=C1C=CC(S(=O)(O)=O)=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (37.38 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4951 mL 7.4756 mL 14.9513 mL
5 mM 0.2990 mL 1.4951 mL 2.9903 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4951 mL 7.4756 mL 14.9513 mL 37.3781 mL
5 mM 0.2990 mL 1.4951 mL 2.9903 mL 7.4756 mL
10 mM 0.1495 mL 0.7476 mL 1.4951 mL 3.7378 mL
15 mM 0.0997 mL 0.4984 mL 0.9968 mL 2.4919 mL
20 mM 0.0748 mL 0.3738 mL 0.7476 mL 1.8689 mL
25 mM 0.0598 mL 0.2990 mL 0.5981 mL 1.4951 mL
30 mM 0.0498 mL 0.2492 mL 0.4984 mL 1.2459 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CY3-YNE
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HY-D0818
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