Dansylamide
Based on 1 Customer Validation
Dansyl amide is a fluorescent dye that is used in biochemistry and chemistry to label substances with the fluorescent dansyl group (Ex/Em: 360 nm/465 nm).
For research use only. We do not sell to patients.
- Purity: 99.28%
- CAS No.: 1431-39-6
- Formula: C12H14N2O2S
- Molecular Weight:250.32
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Dansylamide (DNSA), a site I specific probe, shows a significant increase in the fluorescence intensity of S-homocysteinylated rHSA (Hcys-rHSA)[2].
For the analysis of the binding ability of albumin, HSAs (10 μM) and BD140 (3 μM) or Dansylamide (DNSA) (10 μM) are incubated in phosphate buffer (pH 7.3) at room temperature, and fluorescence measurements are taken after incubation for 30 min[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 1431-39-6
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Appearance Solid
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Molecular Weight 250.32
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Formula C12H14N2O2S
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Color White to off-white
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SMILES
O=S(C1=C2C=CC=C(C2=CC=C1)N(C)C)(N)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (399.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Misra A, et al. Fluorescent labelling of ribonucleosides at 2'-terminus; comparative fluorescence studies. Nucleic Acids Symp Ser. 2000;(44):291-2. [Content Brief]
[2]. Nakashima F, et al. Structural and functional insights into S-thiolation of human serum albumins. Sci Rep. 2018 Jan 17;8(1):932. [Content Brief]
[3]. Tanaka Y, et al. 8-Prenylnaringenin tissue distribution and pharmacokinetics in mice and its binding to human serum albumin and cellular uptake in human embryonic kidney cells. Food Sci Nutr. 2022 Jan 22;10(4):1070-1080. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.9949 mL | 19.9744 mL | 39.9489 mL | 99.8722 mL |
| 5 mM | 0.7990 mL | 3.9949 mL | 7.9898 mL | 19.9744 mL | |
| 10 mM | 0.3995 mL | 1.9974 mL | 3.9949 mL | 9.9872 mL | |
| 15 mM | 0.2663 mL | 1.3316 mL | 2.6633 mL | 6.6581 mL | |
| 20 mM | 0.1997 mL | 0.9987 mL | 1.9974 mL | 4.9936 mL | |
| 25 mM | 0.1598 mL | 0.7990 mL | 1.5980 mL | 3.9949 mL | |
| 30 mM | 0.1332 mL | 0.6658 mL | 1.3316 mL | 3.3291 mL | |
| 40 mM | 0.0999 mL | 0.4994 mL | 0.9987 mL | 2.4968 mL | |
| 50 mM | 0.0799 mL | 0.3995 mL | 0.7990 mL | 1.9974 mL | |
| 60 mM | 0.0666 mL | 0.3329 mL | 0.6658 mL | 1.6645 mL | |
| 80 mM | 0.0499 mL | 0.2497 mL | 0.4994 mL | 1.2484 mL | |
| 100 mM | 0.0399 mL | 0.1997 mL | 0.3995 mL | 0.9987 mL |
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