Gracillin

Name: Gracillin
Brand: MedChemExpress
SKU: HY-N0706
Rating: 5.0 (2 reviews)

Cat. No.: HY-N0706 Purity: 99.91%: Data Sheet
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Gracillin is a steroidal saponin that can be extracted from the roots of the plant and has anti-tumor properties. Gracillin can induce cancer cell apoptosis and autophagy.

For research use only. We do not sell to patients.

CAS No. : 19083-00-2

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Gracillin:

Gracillin (Standard) Get quote

2 Publications Citing Use of MCE Gracillin

Cell Proliferation/Viability Assay

Apoptosis Analysis

Histological Imaging/Staining

In Vivo Efficacy Study

: Gracillin purchased from MedChemExpress. Usage Cited in: Bioorg Chem. 2025 May 29:163:108636.; Cell viability was analyzed in human pancreatic duct (HPNE) and PDAC cells in response to Gracillin. Up to 4 μM, Gracillin did not exhibit cytotoxicity in HPNE cells, while significantly inhibiting PDAC cell viability.

: Gracillin purchased from MedChemExpress. Usage Cited in: Bioorg Chem. 2025 May 29:163:108636.; Immunoblotting analysis confirmed reduced protein expression of PCNA in PDAC cells following Gracillin (0, 1, 2, 4 μM) treatment.

: Gracillin purchased from MedChemExpress. Usage Cited in: Bioorg Chem. 2025 May 29:163:108636.; The apoptosis-inducing effects of Gracillin (0, 1, 2, 4 μM) were confirmed by Annexin V/PI staining in PDAC cells.

: Gracillin purchased from MedChemExpress. Usage Cited in: Bioorg Chem. 2025 May 29:163:108636.; H&E and IHC performed on PANC-1 tumors demonstrating reduced NRF2, HO-1, PCNA, and BCL-2 expression and increased BAX expression following Gracillin treatment.

: Gracillin purchased from MedChemExpress. Usage Cited in: Bioorg Chem. 2025 May 29:163:108636.; Tumor photograph showing the effects of vehicle, Gracillin (8 mg/kg, i.p.) and Gemcitabine treatments.

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Biological Activity
Purity & Documentation
References
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Description

Gracillin is a steroidal saponin that can be extracted from the roots of the plant and has anti-tumor properties. Gracillin can induce cancer cell apoptosis and autophagy^[1]^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	4.3 μM Compound: Gracillin	Cytotoxicity against human A2780 cells after 72 hrs by MTT assay Cytotoxicity against human A2780 cells after 72 hrs by MTT assay	[PMID: 19362474]
A549	IC₅₀	0.96 μM Compound: Gracillin	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 19362474]
BXPC-3	IC₅₀	1.3 μM Compound: Gracillin	Cytotoxicity against human BxPC3 cells after 72 hrs by MTT assay Cytotoxicity against human BxPC3 cells after 72 hrs by MTT assay	[PMID: 19362474]
Bel-7402	IC₅₀	1.1 μM Compound: Gracillin	Cytotoxicity against human Bel7402 cells after 72 hrs by MTT assay Cytotoxicity against human Bel7402 cells after 72 hrs by MTT assay	[PMID: 19362474]
Capan-2	IC₅₀	1.3 μM Compound: Gracillin	Cytotoxicity against human Capan2 cells after 72 hrs by MTT assay Cytotoxicity against human Capan2 cells after 72 hrs by MTT assay	[PMID: 19362474]
HCT-8	IC₅₀	0.92 μM Compound: Gracillin	Cytotoxicity against human HCT8 cells after 72 hrs by MTT assay Cytotoxicity against human HCT8 cells after 72 hrs by MTT assay	[PMID: 19362474]
HL-60	IC₅₀	3.7 μM Compound: 9	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay Cytotoxicity against human HL60 cells after 72 hrs by MTT assay	[PMID: 12828464]
HeLa	IC₅₀	12.74 μg/mL Compound: 8	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 15620239]
MCF7	IC₅₀	17.5 μM Compound: Gracillin	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 19362474]
PANC-1	IC₅₀	0.7 μM Compound: Gracillin	Cytotoxicity against human PANC1 cells after 72 hrs by MTT assay Cytotoxicity against human PANC1 cells after 72 hrs by MTT assay	[PMID: 19362474]
SW1990	IC₅₀	1.1 μM Compound: Gracillin	Cytotoxicity against human SW1990 cells after 72 hrs by MTT assay Cytotoxicity against human SW1990 cells after 72 hrs by MTT assay	[PMID: 19362474]

In Vitro

Gracillin (0-10 μM, 6-24 h) shows broad-spectrum inhibitory effects on the cancer cell viability^[1].
Gracillin (5 μM) disrupts mitochondrial function, specifically, inhibits ATP production, activates AMPK, inhibits oxygen consumption rate (OCR) in H460 and A549 cells^[1].
Gracillin (0-10 μM, 6 h) induces ROS generation (indicated by H2DCF-DA) in H460, H1299, H226B and A549 cells^[1].
Gracillin (0-4 μM, 24 h) blocks cell cycle in G1 phase, and induces apoptosis and autophagy in A549 cells^[2]^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[2]

Cell Line:	A549 cells
Concentration:	0-4 μM
Incubation Time:	24 h
Result:	Induced morphological changes. Apoptosis rate: 6.56%-67.43%.

In Vivo

Gracillin (oral gavage, 10 mg/kg for xenografts and 20 mg/kg for PDX) inhibits tumor growth of xenograft tumors inoculating lung (H1299), prostate (DU145), and colorectum (HCT116) cancer cells, as well as patient-derived xenograft colon and lung tumors^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

885.04

Formula

C₄₅H₇₂O₁₇

CAS No.

19083-00-2

Appearance

Solid

Color

White to light yellow

SMILES

C[C@@]12[C@]([C@@H]3C)([H])[C@](O[C@]34CC[C@@H](C)CO4)([H])C[C@@]1([H])[C@@](CC=C5[C@@]6(CC[C@H](O[C@@](O[C@H](CO)[C@@H](O)[C@@H]7O[C@]([C@@H]([C@@H](O)[C@@H]8O)O)([H])O[C@@H]8CO)([H])[C@@H]7O[C@@](O[C@@H](C)[C@H](O)[C@H]9O)([H])[C@@H]9O)C5)C)([H])[C@]6([H])CC2

Structure Classification

Steroids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (112.99 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.1299 mL	5.6495 mL	11.2989 mL
5 mM	0.2260 mL	1.1299 mL	2.2598 mL
10 mM	0.1130 mL	0.5649 mL	1.1299 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (2.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (255 KB) HNMR (317 KB) RP-HPLC (237 KB) MS (84 KB)

Handling Instructions (2659 KB)

References

[1]. Min HY, et al. The natural compound gracillin exerts potent antitumor activity by targeting mitochondrial complex II. Cell Death Dis. 2019 Oct 24;10(11):810. [Content Brief]

[2]. Yang J, et al. Gracillin Isolated from Reineckia carnea Induces Apoptosis of A549 Cells via the Mitochondrial Pathway. Drug Des Devel Ther. 2021 Jan 20;15:233-243. [Content Brief]

[3]. Li Y, et al. Gracillin Shows Potential Efficacy Against Non-Small Cell Lung Cancer Through Inhibiting the mTOR Pathway. Front Oncol. 2022 Mar 22;12:851300. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.1299 mL	5.6495 mL	11.2989 mL	28.2473 mL
	5 mM	0.2260 mL	1.1299 mL	2.2598 mL	5.6495 mL
	10 mM	0.1130 mL	0.5649 mL	1.1299 mL	2.8247 mL
	15 mM	0.0753 mL	0.3766 mL	0.7533 mL	1.8832 mL
	20 mM	0.0565 mL	0.2825 mL	0.5649 mL	1.4124 mL
	25 mM	0.0452 mL	0.2260 mL	0.4520 mL	1.1299 mL
	30 mM	0.0377 mL	0.1883 mL	0.3766 mL	0.9416 mL
	40 mM	0.0282 mL	0.1412 mL	0.2825 mL	0.7062 mL
	50 mM	0.0226 mL	0.1130 mL	0.2260 mL	0.5649 mL
	60 mM	0.0188 mL	0.0942 mL	0.1883 mL	0.4708 mL
	80 mM	0.0141 mL	0.0706 mL	0.1412 mL	0.3531 mL
	100 mM	0.0113 mL	0.0565 mL	0.1130 mL	0.2825 mL

Gracillin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Gracillin19083-00-2ApoptosisAutophagyInhibitorinhibitorinhibit

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Gracillin

Customer Review

Other Forms of Gracillin:

Gracillin Related Antibodies

2 Publications Citing Use of MCE Gracillin

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Customer Review

Gracillin Related Antibodies

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Complete Stock Solution Preparation Table

Gracillin Related Classifications

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