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  2. Apoptosis Autophagy
  3. Gracillin

Gracillin 

Cat. No.: HY-N0706 Purity: 98.13%
COA Handling Instructions

Gracillin is a steroidal saponin that can be extracted from the roots of the plant and has anti-tumor properties. Gracillin can induce cancer cell apoptosis and autophagy.

For research use only. We do not sell to patients.

Gracillin Chemical Structure

Gracillin Chemical Structure

CAS No. : 19083-00-2

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 92 In-stock
Solution
10 mM * 1 mL in DMSO USD 92 In-stock
Solid
5 mg USD 60 In-stock
10 mg USD 95 In-stock
25 mg USD 175 In-stock
50 mg USD 280 In-stock
100 mg USD 420 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Gracillin

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Gracillin is a steroidal saponin that can be extracted from the roots of the plant and has anti-tumor properties. Gracillin can induce cancer cell apoptosis and autophagy[1][2].

In Vitro

Gracillin (0-10 μM, 6-24 h) shows broad-spectrum inhibitory effects on the cancer cell viability[1].
Gracillin (5 μM) disrupts mitochondrial function, specifically, inhibits ATP production, activates AMPK, inhibits oxygen consumption rate (OCR) ​in H460 and A549 cells[1].
Gracillin (0-10 μM, 6 h) induces ROS generation (indicated by H2DCF-DA) ​in H460, H1299, H226B and A549 cells[1].
Gracillin (0-4 μM, 24 h) blocks cell cycle in G1 phase, and induces apoptosis and autophagy in A549 cells[2][3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis[2]

Cell Line: A549 cells
Concentration: 0-4 μM
Incubation Time: 24 h
Result: Induced morphological changes.
Apoptosis rate: 6.56%-67.43%.
In Vivo

Gracillin (oral gavage, 10 mg/kg for xenografts and 20 mg/kg for PDX) inhibits tumor growth of xenograft tumors inoculating lung (H1299), prostate (DU145), and colorectum (HCT116) cancer cells, as well as patient-derived xenograft colon and lung tumors[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

885.04

Formula

C45H72O17

CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

C[C@@]12[C@]([C@@H]3C)([H])[C@](O[C@]34CC[C@@H](C)CO4)([H])C[C@@]1([H])[C@@](CC=C5[C@@]6(CC[C@H](O[C@@](O[C@H](CO)[C@@H](O)[C@@H]7O[C@]([C@@H]([C@@H](O)[C@@H]8O)O)([H])O[C@@H]8CO)([H])[C@@H]7O[C@@](O[C@@H](C)[C@H](O)[C@H]9O)([H])[C@@H]9O)C5)C)([H])[C@]6([H])CC2

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (112.99 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1299 mL 5.6495 mL 11.2989 mL
5 mM 0.2260 mL 1.1299 mL 2.2598 mL
10 mM 0.1130 mL 0.5649 mL 1.1299 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (2.35 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 98.13%

References
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Gracillin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Gracillin
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HY-N0706
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