1. NF-κB
  2. Keap1-Nrf2

Hesperin 

Cat. No.: HY-101371 Purity: 98.14%
Handling Instructions

Hesperin is a bioactive ingredient present in Japanese horseradish (wasabi) and has been shown to be an Nrf2 activator.

For research use only. We do not sell to patients.

Hesperin Chemical Structure

Hesperin Chemical Structure

CAS No. : 4430-35-7

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 110 In-stock
Stock in the United States
Estimated Time of Arrival: December 31
5 mg USD 100 In-stock
Stock in the United States
Estimated Time of Arrival: December 31
10 mg USD 140 In-stock
Stock in the United States
Estimated Time of Arrival: December 31
50 mg USD 350 In-stock
Stock in the United States
Estimated Time of Arrival: December 31
100 mg USD 550 In-stock
Stock in the United States
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Hesperin is a bioactive ingredient present in Japanese horseradish (wasabi) and has been shown to be an Nrf2 activator.

IC50 & Target

Nrf2[1]

In Vitro

Hesperin (6-Methylsulfinylhexyl isothiocyanate, 6-MSITC) is an active compound in wasabi (Wasabia japonica Matsum.). Whether Hesperin induces cytotoxicity of HUVECs is determined. More than 1 μg/mL of Hesperin markedly induces cytotoxicity and morphological alterations. In subsequent experiments we used Hesperin is used at concentrations of 0-1 μg/mL, to study the anti-coagulant and anti-inflammatory properties of Hesperin in HUVECs[2].

In Vivo

Hesperin (6-Methylsulfinylhexyl isothiocyanate, 6-MSITC) activates Nrf2 and induces phase II enzyme genes but this induction is absent in Nrf2-null mice, suggesting that Hesperin is a potential activator of the Nrf2/ARE-dependent detoxification pathway. To determine whether Hesperin ameliorates hepatic steatosis and iron accumulation, wild-type and Nrf2-null mice are fed the following diets for 12 weeks: 1) control diet, 2) high-fat diet (HFD), 3) HFD plus Hesperin (10 mg/kg/day ip), 4) HFD for 6 weeks followed by an iron-supplemented HFD for 6 weeks (HFD/Iron), 5) HFD/Iron plus Hesperin. The HFD increased hepatic triglycerides in both genotypes and Hesperin suppress increased hepatic triglycerides in wild-type mice but do not reduce these triglycerides in Nrf2-null mice[1].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 4.8700 mL 24.3499 mL 48.6997 mL
5 mM 0.9740 mL 4.8700 mL 9.7399 mL
10 mM 0.4870 mL 2.4350 mL 4.8700 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay
[2]

Primary human umbilical vein endothelial cells (HUVECs) are cultured in collagen-coated tissue-culture dishes in an atmosphere containing 95 % air and 5 % CO2. Human monoblast U937 cells are grown in RPMI-1640 medium with 10 % fetal bovine serum, 10 U/mL Penicillin, and 10μg/mL Streptomycin. HUVECs are cultured in collagen-coated 96-well plates as confluent monolayers. Hesperin is added into wells at the indicated final concentrations (0-30 μg/mL) and then incubated for 24 h. Cell viability is measured by cell counting kits[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Hesperin (6-Methylsulfinylhexyl isothiocyanate, 6-MSITC) is dissolved in 1:10 solution of DMSO/PBS[1].

Mice[1] A colony of wild-type and Nrf2-null mice are backcrossed with C57BL/6 mice for ten generations. All mice are housed in the same animal care facility controlled for temperature, humidity, and light. Seven-week-old male wild-typeand Nrf2-null mice (n=6-8/group) are divided into five groups fed the following diets: 1) a standard diet (AIN-93, containing 4% soybean oil) for 12 weeks and vehicle (1:10 solution of DMSO/PBS) injected intraperitoneally 4 times per week for the last four weeks (control group), 2) a high-fat diet (HFD) (containing 4% soybean oil and 31% lard) for 12 weeks and vehicle injected intraperitoneally 4 times per week for the last four weeks (HFD group),3) a HFD for 12 weeks and Hesperin (10mg/Kg/day; dissolved in 1:10 solution of DMSO/PBS) injected intraperitoneally 4 times per week for the last four weeks (HFD+ Hesperin), 4) a HFD for 6 weeks followed by a HFD containing 1% carbonyl iron for 6 weeks and vehicle injected intraperitoneally 4 times per week for the last four weeks(HFD+Iron), and 5) a HFD for 6 weeks followed by a HFD containing 1% carbonyl iron for 6 weeks and Hesperin (10mg/Kg/day) injected intraperitoneally 4 times per week for the last four weeks (HFD+Iron+Hesperin). After 12 weeks, blood samples are collected by cardiac puncture under anesthesia with sodium pentobarbital (50 mg/kg, ip) and livers are harvested and stored at -80°C until analysis[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

205.34

Formula

C₈H₁₅NOS₂

CAS No.

4430-35-7

SMILES

O=S(CCCCCCN=C=S)C

Storage
Pure form -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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Product Name:
Hesperin
Cat. No.:
HY-101371
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