JAK2 JH2 Tracer
Based on 6 publication(s) in Google Scholar
JAK2 JH2 Tracer (Tracer 5) is a fluorescent probe for JAK2 JH2 domain, with a Kd value of 0.2 μM.
For research use only. We do not sell to patients.
- Purity: 98.03%
- CAS No.: 2101955-00-2
- Formula: C38H27F2N7O6S
- Molecular Weight:747.73
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) JAK2 JH2 Tracer
More
Biological Activity
Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
1. Preparation of working solution
1) Preparation of storage solution
Prepare a 10 mM JAK2 JH2 Tracer stock solution using anhydrous DMSO.
2) Preparation of working solution
Dilute the stored solution with pre-heated serum-free cell culture medium or PBS to prepare a 1.5 pM JAK2 JH2 Tracer working solution.
Note: Please adjust the concentration of JAK2 JH2 Tracer working solution according to the actual situation, and prepare it as needed.
2. Specific staining steps
1) Prepare a series of JAK2 JH2 protein solutions with concentrations ranging from 0 to 4.8 μM in the detection buffer.
2) Add 1.5 pM of JAK2 JH2 Tracer working solution to each well of the microplate.
3) Gradually add the JAK2 JH2 protein solution to the wells containing JAK2 JH2 Tracer, ensuring that the final protein concentration covers the required range.
4) Measure the fluorescence polarization value within 90 minutes.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 2101955-00-2
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Appearance Solid
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Molecular Weight 747.73
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Formula C38H27F2N7O6S
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Color Light brown to brown
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SMILES
S=C(NC1=CC2=C(C3(C4=C(OC5=C3C=CC(O)=C5)C=C(O)C=C4)OC2=O)C=C1)N(CC6=CC=C(NC7=NN(C(C8=C(F)C=CC=C8F)=O)C(N)=N7)C=C6)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (6)
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Journal Impact Factor
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Most Recent
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SLAS Discov
2023 Jun;28(4):180-187. PMID: 37149038 -
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Solvent & Solubility
DMSO : 100 mg/mL (133.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Newton AS, et al. JAK2 JH2 Fluorescence Polarization Assay and Crystal Structures for Complexes with Three Small Molecules. ACS Med Chem Lett. 2017 May 17;8(6):614-617. [Content Brief]
[2]. Ihle JN, Gilliland DG. Jak2: normal function and role in hematopoietic disorders. Curr Opin Genet Dev. 2007 Feb;17(1):8-14. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3374 mL | 6.6869 mL | 13.3738 mL | 33.4345 mL |
| 5 mM | 0.2675 mL | 1.3374 mL | 2.6748 mL | 6.6869 mL | |
| 10 mM | 0.1337 mL | 0.6687 mL | 1.3374 mL | 3.3435 mL | |
| 15 mM | 0.0892 mL | 0.4458 mL | 0.8916 mL | 2.2290 mL | |
| 20 mM | 0.0669 mL | 0.3343 mL | 0.6687 mL | 1.6717 mL | |
| 25 mM | 0.0535 mL | 0.2675 mL | 0.5350 mL | 1.3374 mL | |
| 30 mM | 0.0446 mL | 0.2229 mL | 0.4458 mL | 1.1145 mL | |
| 40 mM | 0.0334 mL | 0.1672 mL | 0.3343 mL | 0.8359 mL | |
| 50 mM | 0.0267 mL | 0.1337 mL | 0.2675 mL | 0.6687 mL | |
| 60 mM | 0.0223 mL | 0.1114 mL | 0.2229 mL | 0.5572 mL | |
| 80 mM | 0.0167 mL | 0.0836 mL | 0.1672 mL | 0.4179 mL | |
| 100 mM | 0.0134 mL | 0.0669 mL | 0.1337 mL | 0.3343 mL |
JAK2 JH2 Tracer Related Classifications
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