Leptin (22-56), human

Name: Leptin (22-56), human
Brand: MedChemExpress
SKU: HY-P1523
Rating: 4.5 (1 reviews)

Cat. No.: HY-P1523 Purity: 98.43%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Leptin (22-56), human is the fragment of leptin, mediated via several isoforms of receptors (Ob-Rs).

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Leptin (22-56), human Chemical Structure

CAS No. : 183598-56-3

Size	Price	Stock	Quantity
500 μg	USD 220	In-stock	Estimated Time of Arrival: December 31
1 mg	USD 360	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 1100	In-stock	Estimated Time of Arrival: December 31
10 mg		Get quote
50 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

Leptin (22-56), human is the fragment of leptin, mediated via several isoforms of receptors (Ob-Rs).

IC₅₀ & Target

Ob-Rs^[1]

In Vitro

Leptin (22-56), human is the fragment of leptin, inhibits corticosterone production, but shows no effect on the proliferation of cultured adrenocortical cells. Leptin (22-56) may be responsible for the direct inhibitory effect on secretion and growth of leptin on cultured rat adrenocortical cells^[1]. Leptin (22-56) inhibits food intake, and may favour erythropoiesis^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

3950.52

Formula

C₁₇₁H₂₉₈N₅₀O₅₆

CAS No.

183598-56-3

Appearance

Solid

Color

White to off-white

Sequence Shortening

VPIQKVQDDTKTLIKTIVTRINDISHTQSVSSKQK

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (25.31 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.2531 mL	1.2657 mL	2.5313 mL
5 mM	0.0506 mL	0.2531 mL	0.5063 mL
10 mM	0.0253 mL	0.1266 mL	0.2531 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (0.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (0.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.43%

Select Batch:

Data Sheet (269 KB) SDS (0 KB)

COA (243 KB) RP-HPLC (113 KB) MS (204 KB)

Handling Instructions (2659 KB)

References

[1]. Malendowicz LK, et al. Effects of leptin and leptin fragments on corticosterone secretion and growth of cultured rat adrenocortical cells. Int J Mol Med. 2004 Nov;14(5):873-7. [Content Brief]

[2]. Stamatiadis DN, et al. Elevated leptin fragments in renal failure correlate with BMI and haematopoiesis and are normalized by haemodialysis. Clin Endocrinol (Oxf). 2004 Apr;60(4):434-41. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.2531 mL	1.2657 mL	2.5313 mL	6.3283 mL
	5 mM	0.0506 mL	0.2531 mL	0.5063 mL	1.2657 mL
	10 mM	0.0253 mL	0.1266 mL	0.2531 mL	0.6328 mL
	15 mM	0.0169 mL	0.0844 mL	0.1688 mL	0.4219 mL
	20 mM	0.0127 mL	0.0633 mL	0.1266 mL	0.3164 mL
	25 mM	0.0101 mL	0.0506 mL	0.1013 mL	0.2531 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Leptin (22-56), human183598-56-3OthersInhibitorinhibitorinhibit

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Leptin (22-56), human

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