1. Epigenetics
  2. MicroRNA

Lin28-let-7a antagonist 1 

Cat. No.: HY-100692
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Lin28-let-7a antagonist 1 shows a clear antagonistic effect against the Lin28-let-7a interaction with an IC50 of 4.03 μM for Lin28A-let-7a-1 interaction.

For research use only. We do not sell to patients.

Lin28-let-7a antagonist 1 Chemical Structure

Lin28-let-7a antagonist 1 Chemical Structure

CAS No. : 2024548-03-4

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Description

Lin28-let-7a antagonist 1 shows a clear antagonistic effect against the Lin28-let-7a interaction with an IC50 of 4.03 μM for Lin28A-let-7a-1 interaction.

IC50 & Target

IC50:4.03 μM (Lin28A-let-7a-1)[1].

In Vitro

Lin28-let-7a antagonist 1 (compound 1) shows a clear antagonistic effect against the Lin28A-let-7a-1 interaction with an IC50 of 4.03 μM. Interestingly, Lin28-let-7a antagonist 1 also inhibits the Lin28B-let-7 interaction, with slightly reduced potency. Dicer processing assay reveals that Lin28-let-7a antagonist 1 clearly blocks the formation of the Lin28A-pre-let-7g complex and induces miRNA processing, resulting in the generation of mature let-7g miRNA. When the expression of both Lin28A and Lin28B are knocked-down using siRNA in JAR cells, the Lin28-let-7a antagonist 1-induced increase in cellular let-7 levels is clearly attenuated. Lin28A-let-7a-IN-1 increases mature let-7 levels in PA-1 cells that express high level of Lin28A. On the other hand, let-7 levels are not affected by 1 in MCF7 cells that rarely express Lin28 proteins. Taken together, Lin28-let-7a antagonist 1 specifically induces the increase in the cellular let-7 levels by targeting Lin28[1].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7135 mL 8.5677 mL 17.1353 mL
5 mM 0.3427 mL 1.7135 mL 3.4271 mL
10 mM 0.1714 mL 0.8568 mL 1.7135 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Molecular Weight

583.59

Formula

C₃₁H₂₉N₅O₇

CAS No.

2024548-03-4

SMILES

O=C(O)C(C=C1)=CC=C1N2N=CC3=C2C4=CC([N+]([O-])=O)=C(N5CCN(C(OCC6=CC=CC=C6)=O)CC5)C=C4OC3(C)C

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
Lin28-let-7a antagonist 1
Cat. No.:
HY-100692
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Lin28-let-7a antagonist 1

Cat. No.: HY-100692