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Lucigenin (Synonyms: NSC-151912; L-6868)

Cat. No.: HY-D0720 Purity: 98.82%
Handling Instructions

Lucigenin(L-6868; NSC-151912) is a chemiluminescent probe used to indicate the presence of endogenously generated superoxide anion radicals in cells.

For research use only. We do not sell to patients.

Lucigenin Chemical Structure

Lucigenin Chemical Structure

CAS No. : 2315-97-1

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 55 In-stock
Estimated Time of Arrival: December 31
Solid
50 mg USD 50 In-stock
Estimated Time of Arrival: December 31
100 mg USD 90 In-stock
Estimated Time of Arrival: December 31
500 mg USD 160 In-stock
Estimated Time of Arrival: December 31
1 g   Get quote  
5 g   Get quote  

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Customer Review

Based on 2 publication(s) in Google Scholar

Top Publications Citing Use of Products
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Description

Lucigenin(L-6868; NSC-151912) is a chemiluminescent probe used to indicate the presence of endogenously generated superoxide anion radicals in cells. Upon single-electron reduction of Lucigenin by a radical species the subsequent form of Lucigenin presents an amplified chemiluminescence which is measurable and can be correlated to the respective amount of reactive radical species present in the system. This approach can be used to probe the natural generation of these species as well as the natural functioning of superoxide dismutase and other enzymes responsible for scavenging radicals. In addition to indicating the presence of radicals in cells, Lucigenin shows interaction with redox-active oxidoreductase enzymes. Lucigenin is shown to mediate the reduction of cytochrome C by xanthine oxidase and to increase the rate of NADPH oxidation. These observations indicate that use of Lucigenin for superoxide analysis is precluded in the presence of high levels of NAD(P)H-oxidoreductases.

Molecular Weight

510.50

Formula

C28H22N4O6

CAS No.
SMILES

C[N+]1=C2C=CC=CC2=C(C3=C4C=CC=CC4=[N+](C)C5=C3C=CC=C5)C6=C1C=CC=C6.O=N([O-])=O.O=N([O-])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (122.43 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9589 mL 9.7943 mL 19.5886 mL
5 mM 0.3918 mL 1.9589 mL 3.9177 mL
10 mM 0.1959 mL 0.9794 mL 1.9589 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (4.07 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (4.07 mM); Clear solution

*All of the co-solvents are available by MCE.
References

Purity: 98.82%

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Product Name:
Lucigenin
Cat. No.:
HY-D0720
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