Mas7
Mas7 (Mastoparan 7), a structural analogue of mastoparan, is an activator of heterotrimeric Gi proteins and its downstream effectors.
For research use only. We do not sell to patients.
- CAS No.: 145854-59-7
- Formula: C67H124N18O15
- Molecular Weight:1421.81
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Mas7 (Mastoparan 7) produces several biological effects in different cell types. The effect of Mas7 on endogenous mono-ADP-ribosyltransferase activity is in the micromolar range with a maximal activation of 205% over the basal. In pertussis treated plasma membranes, it is found that the effect of Mas7 on endogenous mono-ADP-ribosyltransferase is partially blocked, which suggests the involvement of G-proteins, such as Gi or G0[1].
Mas7 is a basic tetradecapeptide isolated from isp venom, which activates guanine nucleotide-binding regulatory proteins (G-proteins) and stimulates apoptosis. In smooth muscle cells, Mas7 leads to an increase in the perfusion pressure. Vascular contraction is induced by Mas7. The vasoconstriction triggered by mas-7 exhibited a slower increase compared to that simulated by phenylephrine or vasopressin[2].
Exposure of hippocampal neurons to a low dose of Mas-7 increases dendritic spine density and spine head width in a time-dependent manner. Additionally, Mas-7 enhances postsynaptic density protein-95 (PSD-95) clustering in neurites and activates Gαo signaling, increasing the intracellular Ca2+ concentration[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 145854-59-7
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Appearance Solid
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Molecular Weight 1421.81
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Formula C67H124N18O15
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Color White to off-white
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Synonyms
Mastoparan 7
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Sequence
Ile-Asn-Leu-Lys-Ala-Leu-Ala-Ala-Leu-Ala-Lys-Ala-Leu-Leu-NH2
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Sequence Shortening
INLKALAALAKALL-NH2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Protocol
Hippocampal neurons cultured in round 35 mm coverslips at a density of 160,000 cells/coverslip are transfected with EGFP at 11 DIV. Then, at 14 DIV the neurons are placed in the imaging chamber in an isotonic solution. The EGFP-positive neurons are imaged with microscope every 5 min for 45 min after the treatment with 1 μM Mas-7. The images are processed and analyzed using ImageJ software[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (263 KB)
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SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Portuguese - PT (419 KB)
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Handling Instructions (2659 KB)
References
[1]. Bavec A, et al. Novel features of amphiphilic peptide Mas7 in signalling via heterotrimeric G-proteins. J Pept Sci. 2004 Nov;10(11):691-9. [Content Brief]
[2]. Grześk G, et al. Direct regulation of vascular smooth muscle contraction by mastoparan-7. Biomed Rep. 2014 Jan;2(1):34-38. [Content Brief]
[3]. Ramírez VT, et al. The Gαo Activator Mastoparan-7 Promotes Dendritic Spine Formation in Hippocampal Neurons. Neural Plast. 2016;2016:4258171. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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