Nec-4
Nec-4, a tricyclic derivative, is a potent receptor interacting protein 1 (RIP1) inhibitor, with an IC50 of 2.6 μM, Ki of 0.46 μM.
For research use only. We do not sell to patients.
- CAS No.: 1041644-43-2
- Formula: C15H13ClFN3O
- Molecular Weight:305.73
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
IC50 & Target
IC50: 2.6 μM (RIP1)[1].
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| Jurkat | EC50 |
0.092 μM
Compound: 48
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Inhibition of TNF-alpha-induced necroptosis in FADD-deficient human Jurkat T cells
Inhibition of TNF-alpha-induced necroptosis in FADD-deficient human Jurkat T cells
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[PMID: 18467094] |
In Vitro
Nec-4 (compound 9) is a slightly better competitor than 7 with a lower Ki value that is similar to R-3. Nec-4 is again a slightly better competitor than Rac-3 with Ki and IC50 values comparable to 7 (Nec-4: IC50=2.6±0.1 μM, Ki=0.46±0.05 μM; 7: IC50=10.7±1.8 μM, Ki=4.5±0.9 μM). Overall, all three compounds cross-compete with each other but Nec-4 is able to more effectively displace both 20 and 26 in comparison to the parent compounds Rac-3 and 7, respectively. This suggests that Nec-4 binding likely significantly overlaps with both the Nec-1 and the Nec-3 binding sites.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1041644-43-2
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Molecular Weight 305.73
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Formula C15H13ClFN3O
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SMILES
C[C@@H](C1=C(C=CC=C1Cl)F)NC(C2=CC=C(N2C)C#N)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Nec-4 Related Classifications
HY-18900 Related Classifications
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