Cat. No.: HY-N0101

Neohesperidin

(Synonyms: Hesperetin 7-O-neohesperidoside)

9 Cited Publications

Customer Review

Based on 9 publication(s) in Google Scholar

Neohesperidin is a flavonoid compound abundant in citrus plants with antioxidant and anti-inflammatory effects. Neohesperidin can upregulate the Wnt/β-catenin signaling pathway, enhance the nuclear translocation of β-catenin and the differentiation of bone marrow stromal cells.

For research use only. We do not sell to patients.

Purity: 98.67%
CAS No.: 13241-33-3
Formula: C₂₈H₃₄O₁₅
Molecular Weight:610.56

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Neohesperidin Chemical Structure

CAS No.: 13241-33-3

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (9)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Wnt Isoforms :

In Vitro

Neohesperidin induces cell apoptosis in human breast adenocarcinoma MDA-MB-231 cells. The IC₅₀ values of neohesperidin at 24 and 48 h are 47.4±2.6 μM and 32.5±1.8 μM, respectively. The expressions of P53 and Bax in the neohesperidin-treated cells are significantly up-regulated, while that of Bcl-2 is down-regulated^[1]. Neohesperidin exhibits antioxidant activity (IC₅₀=22.31 μg/mL) in the DPPH radical-scavenging assay^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Neohesperidin (50 mg/kg) significantly inhibits 55.0% of HCl/ethanol-induced gastric lesions. In pylorus ligated rats, neohesperidin (50 mg/kg) significantly decreases the volume of gastric secretion and gastric acid output, and increases the pH^[1]. Treatment of neohesperidin significantly decreases fasting glucose, serum glucose, and glycosylated serum protein (GSP) in mice. It significantly elevates oral glucose tolerance and insulin sensitivity and decreases insulin resistance in the diabetic mice. Neohesperidin significantly decreases serum triglycerides, total cholesterol, leptin level, and liver index in the mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 13241-33-3
Appearance Solid
Molecular Weight 610.56
Formula C₂₈H₃₄O₁₅
Color White to off-white
SMILES

O=C1C[C@@H](C2=CC=C(OC)C(O)=C2)OC3=CC(O[C@H]4[C@@H]([C@H]([C@@H]([C@@H](CO)O4)O)O)O[C@@]5([H])[C@@H]([C@@H]([C@H]([C@H](C)O5)O)O)O)=CC(O)=C13
Synonyms

Hesperetin 7-O-neohesperidoside
Structure Classification
- Flavonoids
- Flavonones
- Phenols
- Polyphenols
Initial Source
- Plants
- Rutaceae
- Citrus
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (9)

All Cell Proliferation/Viability AssayWBELISACell Migration/Invasion AssayApoptosis Analysis

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Journal Impact Factor

Acta Pharm Sin B

Chrysin serves as a novel inhibitor of DGK α/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC). [Abstract]

2021 Jan;11(1):143-155. PMID: 33532186
Phytomedicine

Novel Flavan-3,4-diol vernicidin B from Toxicodendron Vernicifluum (Anacardiaceae) as potent antioxidant via IL-6/Nrf2 cross-talks pathways. [Abstract]

2022 Jun:100:154041. PMID: 35306369
Food Chem

Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]

2025 Dec 30:497:146992. PMID: 41285060
Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 May 31:489:144992. PMID: 40466530
Phytother Res

Neohesperidin Regulates Lipid Metabolism and Ameliorates MASLD by Targeting ECHS1. [Abstract]

2026 Jun 29. PMID: 42370492
Chin J Nat Med

Therapeutic effect of neohesperidin on TNF-α-stimulated human rheumatoid arthritis fibroblast-like synoviocytes. [Abstract]

2021 Oct;19(10):741-749. PMID: 34688464

Neohesperidin purchased from MedChemExpress. Usage Cited in: Chin J Nat Med. 2021 Oct;19(10):741-749. [Abstract]

RA-FLSs in 96-well plates were treated with different concentrations of Neohesperidin (Neo) (0.0, 10.0, 20.0, 30.0, 40.0, 50.0, 60.0 and 70.0 μM) for 24 h. The medium was removed and cell viability was analyzed by CCK-8 assay.

Neohesperidin purchased from MedChemExpress. Usage Cited in: Chin J Nat Med. 2021 Oct;19(10):741-749. [Abstract]

The amounts of MMP3, MMP9 and MMP-13 in RA-FLSs were measured by Western blot treated with Neohesperidin (NEO) (5, 10, 30 μM).

Neohesperidin purchased from MedChemExpress. Usage Cited in: Chin J Nat Med. 2021 Oct;19(10):741-749. [Abstract]

The levels of IL-1β, IL-6, IL-8 and TNF-α in the supernatant of RAFLSs were measured by ELISA treated with Neohesperidin (NEO) (5, 10, 30 μM).

Neohesperidin purchased from MedChemExpress. Usage Cited in: Chin J Nat Med. 2021 Oct;19(10):741-749. [Abstract]

Images showing the migration of RA-FLSs treated with different concentrations of Neohesperidin (NEO) (5.0, 10.0, and 30.0 μM) 24 h and 36 h after scratching.

Neohesperidin purchased from MedChemExpress. Usage Cited in: Chin J Nat Med. 2021 Oct;19(10):741-749. [Abstract]

Cell apoptotic rate was detected by flow cytometry with Annexin V-FITC/PI double staining treated with Neohesperidin (NEO) (5, 10, 30 μM).
J Photochem Photobiol B

Spectral and color temperature dependence of LED exposure on retinal damage and the protective effects of neohesperidin. [Abstract]

2025 May:266:113148. PMID: 40073747
Int J Food Sci Technol

2023 Aug 31.
Fitoterapia

Maizediterpene D from the roots of Zea mays L. alleviates hydrogen peroxide induced oxidative stress and improves cell survival by activation of TrkB/IGF-1R crosstalk pathways. [Abstract]

2022 Nov:163:105314. PMID: 36174847

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (163.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.6378 mL

8.1892 mL

16.3784 mL

5 mM

0.3276 mL

1.6378 mL

3.2757 mL

10 mM

0.1638 mL

0.8189 mL

1.6378 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (4.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.5% CMC-Na/saline water

Solubility: 5 mg/mL (8.19 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

Animal Administration^[3]

Mice: All the mice are fasted 6 h before the test and then fed with water or neohesperidin by gavage. The mice are intraperitoneally injected with either 2 g/kg BW glucose or 1 IU/kg BW insulin for OGTT and ITT, respectively. Blood samples are collected from the tail vein for the measurement of basal blood glucose levels (0 min) before the injection of glucose or insulin. Additional blood glucose levels are measured at 30, 60, 90 and 120 min^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Yuan S, et al. Neohesperidin promotes the proliferation and osteogenic differentiation of BMSCs via BMP2-Wnt/β-catenin pathway. Cell Cycle. 2022 Jan;21(2):187-201. [Content Brief]

[2]. Lee JH, et al. Protective effects of neohesperidin and poncirin isolated from the fruits of Poncirus trifoliata on potential gastric disease. Phytother Res. 2009 Dec;23(12):1748-53. [Content Brief]

[3]. Xu F, et al. Neohesperidin induces cellular apoptosis in human breast adenocarcinoma MDA-MB-231 cells via activating the Bcl-2/Bax-mediated signaling pathway. Nat Prod Commun. 2012 Nov;7(11):1475-8. [Content Brief]

[4]. Jia S, et al. Hypoglycemic and hypolipidemic effects of neohesperidin derived from Citrus aurantium L. in diabetic KK-A(y) mice. Food Funct. 2015 Mar;6(3):878-86. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.6378 mL	8.1892 mL	16.3784 mL	40.9460 mL
	5 mM	0.3276 mL	1.6378 mL	3.2757 mL	8.1892 mL
	10 mM	0.1638 mL	0.8189 mL	1.6378 mL	4.0946 mL
	15 mM	0.1092 mL	0.5459 mL	1.0919 mL	2.7297 mL
	20 mM	0.0819 mL	0.4095 mL	0.8189 mL	2.0473 mL
	25 mM	0.0655 mL	0.3276 mL	0.6551 mL	1.6378 mL
	30 mM	0.0546 mL	0.2730 mL	0.5459 mL	1.3649 mL
	40 mM	0.0409 mL	0.2047 mL	0.4095 mL	1.0237 mL
	50 mM	0.0328 mL	0.1638 mL	0.3276 mL	0.8189 mL
	60 mM	0.0273 mL	0.1365 mL	0.2730 mL	0.6824 mL
	80 mM	0.0205 mL	0.1024 mL	0.2047 mL	0.5118 mL
	100 mM	0.0164 mL	0.0819 mL	0.1638 mL	0.4095 mL

Keywords

Neohesperidin

9 Cited Publications

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Neohesperidin

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Publications Citing Use of MedChemExpress (MCE) Neohesperidin

All Wnt Isoforms

Biological Activity

Chemical Information

Publications (9)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Neohesperidin Related Classifications

HY-N0101 Related Classifications