2. Stem Cell/Wnt
  3. Oct3/4
  4. O4I1

O4I1 is an Oct3/4 modulator with activity toward induced-pluripotent stem cell generation.O4I1 enhances Oct3/4 expression, increases Oct3/4 protein levels, and promotes Oct3/4-mediated transcriptional activity.O4I1 can be used for the research of regenerative medicine.

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O4I1

O4I1 Chemical Structure

CAS No. : 175135-47-4

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
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O4I1 Related Antibodies

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Description

O4I1 is an Oct3/4 modulator with activity toward induced-pluripotent stem cell generation.O4I1 enhances Oct3/4 expression, increases Oct3/4 protein levels, and promotes Oct3/4-mediated transcriptional activity.O4I1 can be used for the research of regenerative medicine[1].

In Vitro

O4I1 (5-20 μM; 1-72 h) potently activates the Oct3/4 promoter in HEK-Oct3/4 cells. After treatment with 5 μM for 72 h, luciferase activity increases to 3-fold of the original level, and the induction shows a sustained, concentration-dependent pattern over time[1].
O4I1 (10-20 μM; 72 h) upregulates the expression of endogenous Oct3/4 mRNA in HEK293 cells, with a 4-fold increase in expression observed after treatment with 20 μM for 72 h[1].
O4I1 (24-96 h) upregulates the protein levels of Oct3/4, Sox2 and Nanog in HEK293 cells in a concentration- and time-dependent manner in vitro. Specifically, Oct3/4 begins to accumulate after treatment with 6 μM for 48 h, and this accumulation persists for at least 96 h under treatment with 10 μM[1].
O4I1 (10 μM; 3-7 days) induces terminally differentiated human fibroblasts to express Oct3/4 protein, including HF cells after 72 h of treatment and HFF cells after 7 days of treatment[1].
O4I1 (10 μM; 72 h) activates the transcriptional activity of Oct3/4 in NCCIT-Oct3/4 cells, increasing luciferase activity by 2.68-fold after treatment at 10 μM for 72 h[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

O4I1 Related Antibodies

Real Time qPCR[1]

Cell Line: HEK293 cells
Concentration: 10 μM, 20 μM
Incubation Time: 72 h
Result: Increased Oct3/4 mRNA levels by approximately 2.5-fold at 10 μM relative to DMSO control after 72 h of treatment.
Increased Oct3/4 mRNA levels by 4-fold at 20 μM relative to DMSO control after 72 h of treatment.

Western Blot Analysis[1]

Cell Line: human adult primary fibroblasts (HF), human neonatal foreskin fibroblasts (HFF)
Concentration: 10 μM
Incubation Time: 72 h (HF); 7 days (HFF)
Result: Detected Oct3/4 protein induction in HF cells after 72 h of treatment with 10 μM O4I1.
Detected Oct3/4 protein induction in HFF cells after 7 days of treatment with 10 μM O4I1.
Molecular Weight

253.30

Formula

C16H15NO2
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

N#CCC1=CC=C(OCC2=CC=C(OC)C=C2)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 38 mg/mL (150.02 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.9479 mL 19.7394 mL 39.4789 mL
5 mM 0.7896 mL 3.9479 mL 7.8958 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.9479 mL 19.7394 mL 39.4789 mL 98.6972 mL
5 mM 0.7896 mL 3.9479 mL 7.8958 mL 19.7394 mL
10 mM 0.3948 mL 1.9739 mL 3.9479 mL 9.8697 mL
15 mM 0.2632 mL 1.3160 mL 2.6319 mL 6.5798 mL
20 mM 0.1974 mL 0.9870 mL 1.9739 mL 4.9349 mL
25 mM 0.1579 mL 0.7896 mL 1.5792 mL 3.9479 mL
30 mM 0.1316 mL 0.6580 mL 1.3160 mL 3.2899 mL
40 mM 0.0987 mL 0.4935 mL 0.9870 mL 2.4674 mL
50 mM 0.0790 mL 0.3948 mL 0.7896 mL 1.9739 mL
60 mM 0.0658 mL 0.3290 mL 0.6580 mL 1.6450 mL
80 mM 0.0493 mL 0.2467 mL 0.4935 mL 1.2337 mL
100 mM 0.0395 mL 0.1974 mL 0.3948 mL 0.9870 mL
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O4I1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

O4I1175135-47-4Oct3/4POU5F1Slc22a3Sox2induced-pluripotent stem cellHEK-Oct3/4 cellsNCCIT-Oct3/4 cellsHEK293 cellsHF cellsHFF cellsNanoghuman fibroblastsInhibitorinhibitorinhibit

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