O4I1
Based on 1 Customer Validation
O4I1 is an Oct3/4 modulator with activity toward induced-pluripotent stem cell generation.O4I1 enhances Oct3/4 expression, increases Oct3/4 protein levels, and promotes Oct3/4-mediated transcriptional activity.O4I1 can be used for the research of regenerative medicine.
For research use only. We do not sell to patients.
- Purity: 98.05%
- CAS No.: 175135-47-4
- Formula: C16H15NO2
- Molecular Weight:253.30
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
O4I1 (5-20 μM; 1-72 h) potently activates the Oct3/4 promoter in HEK-Oct3/4 cells. After treatment with 5 μM for 72 h, luciferase activity increases to 3-fold of the original level, and the induction shows a sustained, concentration-dependent pattern over time[1].
O4I1 (10-20 μM; 72 h) upregulates the expression of endogenous Oct3/4 mRNA in HEK293 cells, with a 4-fold increase in expression observed after treatment with 20 μM for 72 h[1].
O4I1 (24-96 h) upregulates the protein levels of Oct3/4, Sox2 and Nanog in HEK293 cells in a concentration- and time-dependent manner in vitro. Specifically, Oct3/4 begins to accumulate after treatment with 6 μM for 48 h, and this accumulation persists for at least 96 h under treatment with 10 μM[1].
O4I1 (10 μM; 3-7 days) induces terminally differentiated human fibroblasts to express Oct3/4 protein, including HF cells after 72 h of treatment and HFF cells after 7 days of treatment[1].
O4I1 (10 μM; 72 h) activates the transcriptional activity of Oct3/4 in NCCIT-Oct3/4 cells, increasing luciferase activity by 2.68-fold after treatment at 10 μM for 72 h[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HEK293 cells
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Concentration:10 μM, 20 μM
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Incubation Time:72 h
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Result:Increased Oct3/4 mRNA levels by approximately 2.5-fold at 10 μM relative to DMSO control after 72 h of treatment.
Increased Oct3/4 mRNA levels by 4-fold at 20 μM relative to DMSO control after 72 h of treatment.
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Cell Line:human adult primary fibroblasts (HF), human neonatal foreskin fibroblasts (HFF)
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Concentration:10 μM
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Incubation Time:72 h (HF); 7 days (HFF)
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Result:Detected Oct3/4 protein induction in HF cells after 72 h of treatment with 10 μM O4I1.
Detected Oct3/4 protein induction in HFF cells after 7 days of treatment with 10 μM O4I1.
Chemical Information
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CAS No. 175135-47-4
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Appearance Solid
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Molecular Weight 253.30
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Formula C16H15NO2
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Color Light yellow to yellow
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SMILES
N#CCC1=CC=C(OCC2=CC=C(OC)C=C2)C=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : ≥ 38 mg/mL (150.02 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (270 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.9479 mL | 19.7394 mL | 39.4789 mL | 98.6972 mL |
| 5 mM | 0.7896 mL | 3.9479 mL | 7.8958 mL | 19.7394 mL | |
| 10 mM | 0.3948 mL | 1.9739 mL | 3.9479 mL | 9.8697 mL | |
| 15 mM | 0.2632 mL | 1.3160 mL | 2.6319 mL | 6.5798 mL | |
| 20 mM | 0.1974 mL | 0.9870 mL | 1.9739 mL | 4.9349 mL | |
| 25 mM | 0.1579 mL | 0.7896 mL | 1.5792 mL | 3.9479 mL | |
| 30 mM | 0.1316 mL | 0.6580 mL | 1.3160 mL | 3.2899 mL | |
| 40 mM | 0.0987 mL | 0.4935 mL | 0.9870 mL | 2.4674 mL | |
| 50 mM | 0.0790 mL | 0.3948 mL | 0.7896 mL | 1.9739 mL | |
| 60 mM | 0.0658 mL | 0.3290 mL | 0.6580 mL | 1.6450 mL | |
| 80 mM | 0.0493 mL | 0.2467 mL | 0.4935 mL | 1.2337 mL | |
| 100 mM | 0.0395 mL | 0.1974 mL | 0.3948 mL | 0.9870 mL |
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