1. Apoptosis
    Cytoskeleton
    Cell Cycle/DNA Damage
  2. Apoptosis
    Microtubule/Tubulin

PBOX 6 

Cat. No.: HY-U00446 Purity: >98.0%
Handling Instructions

PBOX 6 is a pyrrolo-1,5-benzoxazepine (PBOX) compound, acts as a microtubule-depolymerizing agent and an apoptotic agent.

For research use only. We do not sell to patients.

PBOX 6 Chemical Structure

PBOX 6 Chemical Structure

CAS No. : 290814-68-5

Size Price Stock Quantity
5 mg USD 160 In-stock
Estimated Time of Arrival: December 31
10 mg USD 290 In-stock
Estimated Time of Arrival: December 31
25 mg USD 590 Get quote
50 mg   Get quote  
100 mg   Get quote  

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

PBOX 6 is a pyrrolo-1,5-benzoxazepine (PBOX) compound, acts as a microtubule-depolymerizing agent and an apoptotic agent.

IC50 & Target

Apoptosis[1], Microtubule[3]

In Vitro

PBOX 6 is a potent apoptotic PBOX, but does not elicit a general toxic effect in a rat R2C Leydig cell line. PBOX 6 (0-25 μM, 16 h) results in dose- and time-dependent induction of apoptosis, and also causes DNA fragmentation at 10 μM in HL-60 cells. PBOX 6 (10 μM) induces apoptosis through activation of caspase 3-like proteases in HL-60 cells. PBOX 6 (10 μM) induces apoptosis and exerts an accumulation of cytochromec in the cytosol, but this effect is not triggered by oxidative stress, and is independent of peripheral-type benzodiazepine receptor (PBR) and NF-κB[1]. PBOX 6 (25 μM) induces apoptosis in MCF-7 cells through activation of caspase-7[2]. PBOX 6 (10 μM) induces the redistribution of cypA from the nucleus to the cytosol of the cell in K562 cells. PBOX 6 (10 μM) induces nucleocytoplasmic redistribution of cypA and pin1 through a JNK-dependent manner, also dependent on upstream activation of a trypsin-like serine protease, and this effect correlates with G2/M arrest in K562 cells[3].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5224 mL 12.6122 mL 25.2245 mL
5 mM 0.5045 mL 2.5224 mL 5.0449 mL
10 mM 0.2522 mL 1.2612 mL 2.5224 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

Cells are seeded at a density of 3 × 105 cells/mL and following treatment with the indicated compound (including PBOX 6), an aliquot (100 μL) is cytocentrifuged onto glass slides precoated with poly(l-lysine). They are then stained with the RapiDiff kit (eosin/methylene blue). The degree of apoptosis and necrosis is determined by counting 300 cells under a light microscope. At least three fields of view per slide, with an average of 100 cells per field, are counted and the percentage of apoptosis and necrosis is determined. Apoptotic cells are characterized by cell shrinkage, membrane blebbing, and nuclear condensation and fragmentation, whereas necrotic cells are identified by cell swelling and loss of cell membrane[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

396.44

Formula

C₂₅H₂₀N₂O₃

CAS No.

290814-68-5

SMILES

O=C(OC1=C(C2=C3C=CC=CC3=CC=C2)OC4=CC=CC=C4N5C1=CC=C5)N(C)C

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Purity: >98.0%

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Product Name:
PBOX 6
Cat. No.:
HY-U00446
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PBOX 6

Cat. No.: HY-U00446