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  2. Photo-lysine

Photo-lysine (Synonyms: Photo lysine)

Cat. No.: HY-19804
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Photo-lysine, a new lysine-based photo-reactive amino acid, captures proteins that bind lysine post-translational modifications.

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Photo-lysine Chemical Structure

Photo-lysine Chemical Structure

CAS No. : 1863117-91-2

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Description

Photo-lysine, a new lysine-based photo-reactive amino acid, captures proteins that bind lysine post-translational modifications.

In Vitro

Photo-lysine is designed and synthesized by incorporating a photo-cross-linker (diazirine) into the side chain of natural lysine. Photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine post-translational modifications (PTMs), including ‘readers’ and ‘erasers’ of histone modifications. Photo-lysine can be incorporated into MDH2 and mediate photo-cross-linking to fix protein-protein interactions in cells. UV irradiation of cells in the presence of photo-lysine induced robust cross-linking of HSP90β and HSP60. Photo-lysine has higher efficiency than photo-leucine for photo-cross-linking of the two chaperone proteins. Photo-lysine enables capture of the heterodimer of proteins Ku70 and Ku80 within a protein complex. Photo-lysine enables identification of histone- and chromatin-binding proteins[1].

Molecular Weight

172.19

Formula

C₆H₁₂N₄O₂

CAS No.

1863117-91-2

SMILES

NCCC1(N=N1)C[[email protected]@H](C(O)=O)N

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Keywords:

Photo-lysinePhoto lysineOthersInhibitorinhibitorinhibit

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