Photo-lysine hydrochloride
Based on 1 publication(s) in Google Scholar
Photo-lysine hydrochloride, a new lysine-based photo-reactive amino acid, captures proteins that bind lysine post-translational modifications.
For research use only. We do not sell to patients.
- Formula: C6H14Cl2N4O2
- Molecular Weight:245.11
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Photo-lysine hydrochloride
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Biological Activity
Photo-lysine is designed and synthesized by incorporating a photo-cross-linker (diazirine) into the side chain of natural lysine. Photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine post-translational modifications (PTMs), including ‘readers’ and ‘erasers’ of histone modifications. Photo-lysine can be incorporated into MDH2 and mediate photo-cross-linking to fix protein-protein interactions in cells. UV irradiation of cells in the presence of photo-lysine induced robust cross-linking of HSP90β and HSP60. Photo-lysine has higher efficiency than photo-leucine for photo-cross-linking of the two chaperone proteins. Photo-lysine enables capture of the heterodimer of proteins Ku70 and Ku80 within a protein complex. Photo-lysine enables identification of histone- and chromatin-binding proteins[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 245.11
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Formula C6H14Cl2N4O2
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Color White to off-white
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SMILES
O=C(O)[C@@H]([NH3+])CC1(N=N1)CC[NH3+].[Cl-].[Cl-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (1)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
H2O : ≥ 40 mg/mL (163.19 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 4.0798 mL | 20.3990 mL | 40.7980 mL | 101.9950 mL |
| 5 mM | 0.8160 mL | 4.0798 mL | 8.1596 mL | 20.3990 mL | |
| 10 mM | 0.4080 mL | 2.0399 mL | 4.0798 mL | 10.1995 mL | |
| 15 mM | 0.2720 mL | 1.3599 mL | 2.7199 mL | 6.7997 mL | |
| 20 mM | 0.2040 mL | 1.0200 mL | 2.0399 mL | 5.0998 mL | |
| 25 mM | 0.1632 mL | 0.8160 mL | 1.6319 mL | 4.0798 mL | |
| 30 mM | 0.1360 mL | 0.6800 mL | 1.3599 mL | 3.3998 mL | |
| 40 mM | 0.1020 mL | 0.5100 mL | 1.0200 mL | 2.5499 mL | |
| 50 mM | 0.0816 mL | 0.4080 mL | 0.8160 mL | 2.0399 mL | |
| 60 mM | 0.0680 mL | 0.3400 mL | 0.6800 mL | 1.6999 mL | |
| 80 mM | 0.0510 mL | 0.2550 mL | 0.5100 mL | 1.2749 mL | |
| 100 mM | 0.0408 mL | 0.2040 mL | 0.4080 mL | 1.0200 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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