Photo-lysine hydrochloride

Name: Photo-lysine hydrochloride
Brand: MedChemExpress
SKU: HY-19804A
Rating: 4.5 (1 reviews)

Cat. No.: HY-19804A Purity: 99.25%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Photo-lysine hydrochloride, a new lysine-based photo-reactive amino acid, captures proteins that bind lysine post-translational modifications.

For research use only. We do not sell to patients.

Photo-lysine hydrochloride Chemical Structure

Size	Price	Stock	Quantity
1 mg	USD 545	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 1200	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 2160	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Photo-lysine hydrochloride:

Photo-lysine Get quote

1 Publications Citing Use of MCE Photo-lysine hydrochloride

•bioRxiv. 2020 Feb.

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•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

Photo-lysine hydrochloride, a new lysine-based photo-reactive amino acid, captures proteins that bind lysine post-translational modifications.

In Vitro

Photo-lysine is designed and synthesized by incorporating a photo-cross-linker (diazirine) into the side chain of natural lysine. Photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine post-translational modifications (PTMs), including ‘readers’ and ‘erasers’ of histone modifications. Photo-lysine can be incorporated into MDH2 and mediate photo-cross-linking to fix protein-protein interactions in cells. UV irradiation of cells in the presence of photo-lysine induced robust cross-linking of HSP90β and HSP60. Photo-lysine has higher efficiency than photo-leucine for photo-cross-linking of the two chaperone proteins. Photo-lysine enables capture of the heterodimer of proteins Ku70 and Ku80 within a protein complex. Photo-lysine enables identification of histone- and chromatin-binding proteins^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

245.11

Formula

C₆H₁₄Cl₂N₄O₂

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)[C@@H]([NH3+])CC1(N=N1)CC[NH3+].[Cl-].[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

H₂O : ≥ 40 mg/mL (163.19 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0798 mL	20.3990 mL	40.7980 mL
5 mM	0.8160 mL	4.0798 mL	8.1596 mL
10 mM	0.4080 mL	2.0399 mL	4.0798 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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mg/kg

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μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.25%

Select Batch:

Data Sheet (271 KB) SDS (251 KB)

COA (246 KB) RP-HPLC (219 KB)

Handling Instructions (2659 KB)

References

[1]. Yang T, et al. Photo-lysine captures proteins that bind lysine post-translational modifications. Nat Chem Biol. 2016 Feb;12(2):70-2. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	4.0798 mL	20.3990 mL	40.7980 mL	101.9950 mL
	5 mM	0.8160 mL	4.0798 mL	8.1596 mL	20.3990 mL
	10 mM	0.4080 mL	2.0399 mL	4.0798 mL	10.1995 mL
	15 mM	0.2720 mL	1.3599 mL	2.7199 mL	6.7997 mL
	20 mM	0.2040 mL	1.0200 mL	2.0399 mL	5.0998 mL
	25 mM	0.1632 mL	0.8160 mL	1.6319 mL	4.0798 mL
	30 mM	0.1360 mL	0.6800 mL	1.3599 mL	3.3998 mL
	40 mM	0.1020 mL	0.5100 mL	1.0200 mL	2.5499 mL
	50 mM	0.0816 mL	0.4080 mL	0.8160 mL	2.0399 mL
	60 mM	0.0680 mL	0.3400 mL	0.6800 mL	1.6999 mL
	80 mM	0.0510 mL	0.2550 mL	0.5100 mL	1.2749 mL
	100 mM	0.0408 mL	0.2040 mL	0.4080 mL	1.0200 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.