TNP-470 (Synonyms: AGM-1470)

Name: TNP-470
Brand: MedChemExpress
SKU: HY-101932
Rating: 4.5 (1 reviews)

製品番号: HY-101932 純度: 99.87%: Data Sheet SDS COA 取扱説明書
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Technical Support

TNP-470 is a methionine aminopeptidase-2 inhibitor and also an angiogenesis inhibitor.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
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TNP-470 構造式

CAS 番号 : 129298-91-5

容量	価格（税別）	在庫状況	数量
Solid or liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 155	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 155	在庫あり	Estimated Time of Arrival: December 31
Solid or liquid
1 mg	USD 88	在庫あり	Estimated Time of Arrival: December 31
5 mg	USD 175	在庫あり	Estimated Time of Arrival: December 31
10 mg	USD 280	在庫あり	Estimated Time of Arrival: December 31
50 mg		お問い合わせ
100 mg		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 1 publication(s) in Google Scholar

顧客検証

•Exp Mol Med. 2024 Aug 1. [Abstract]

Aminopeptidase アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

CD13 Leukotriene A4 hydrolase

生物活性
プロトコル
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

TNP-470 is a methionine aminopeptidase-2 inhibitor and also an angiogenesis inhibitor.

IC₅₀ & Target

methionine aminopeptidase-2^[1], angiogenesis^[2]

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	50 μM Compound: TNP470	Antiproliferative activity human A549 cells after 20 hrs by MTT assay Antiproliferative activity human A549 cells after 20 hrs by MTT assay	[PMID: 20149494]
Bel-7402	IC₅₀	≥ 50 μM Compound: TNP470	Antiproliferative activity human Bel7402 cells after 20 hrs by MTT assay Antiproliferative activity human Bel7402 cells after 20 hrs by MTT assay	[PMID: 20149494]
BGC-823	IC₅₀	1.48 μM Compound: TNP-470	Antiproliferative activity against human BGC823 cells after 24 hrs by MTT assay Antiproliferative activity against human BGC823 cells after 24 hrs by MTT assay	[PMID: 21925884]
CPAE	IC₅₀	0.04 ng/mL Compound: 7	Tested in vitro for its ability to inhibit cell proliferation of calf pulmonary artery endothelial cells measured calorimetrically by SRB method Tested in vitro for its ability to inhibit cell proliferation of calf pulmonary artery endothelial cells measured calorimetrically by SRB method	[PMID: 10636239]
EL4	IC₅₀	0.03 ng/mL Compound: 7	Tested in vitro for its ability to inhibit cell proliferation of lymphoma EL-4 cells measured calorimetrically by MTT method Tested in vitro for its ability to inhibit cell proliferation of lymphoma EL-4 cells measured calorimetrically by MTT method	[PMID: 10636239]
HeLa	IC₅₀	2.02 μM Compound: TNP-470	Antiproliferative activity against human HeLa cells after 24 hrs by MTT assay Antiproliferative activity against human HeLa cells after 24 hrs by MTT assay	[PMID: 21925884]
HepG2	IC₅₀	0.86 μM Compound: TNP-470	Antiproliferative activity against human HepG2 cells after 24 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 24 hrs by MTT assay	[PMID: 21925884]
HUVEC	IC₅₀	0.0089 μM Compound: TNP470	Antiproliferative activity HUVEC after 20 hrs by MTT assay Antiproliferative activity HUVEC after 20 hrs by MTT assay	[PMID: 20149494]
HUVEC	IC₅₀	0.2 nM Compound: 2a	Growth inhibition of HUVEC cells by XTT assay Growth inhibition of HUVEC cells by XTT assay	[PMID: 17636946]
HUVEC	IC₅₀	0.5 μM Compound: TNP-470	Antiangiogenic activity against HUVEC assessed as inhibition of VEGF-induced cell migration by NCI antiangiogenesis assay Antiangiogenic activity against HUVEC assessed as inhibition of VEGF-induced cell migration by NCI antiangiogenesis assay	[PMID: 19084416]
HUVEC	IC₅₀	0.76 nM Compound: TNP470	Antiproliferative activity against human HUVEC cells after 96 hrs by MTT assay Antiproliferative activity against human HUVEC cells after 96 hrs by MTT assay	[PMID: 21296467]
HUVEC	IC₅₀	1 μM Compound: TNP-470	Antiangiogenic activity against HUVEC assessed as inhibition of capillary-like structure formation by NCI antiangiogenesis assay Antiangiogenic activity against HUVEC assessed as inhibition of capillary-like structure formation by NCI antiangiogenesis assay	[PMID: 19084416]
HUVEC	IC₅₀	1.96 μM Compound: TNP-470	Antiproliferative against HUVEC after 48 hrs by MTT assay Antiproliferative against HUVEC after 48 hrs by MTT assay	[PMID: 23582273]
HUVEC	IC₅₀	3.16 μM Compound: TNP-470	Antiangiogenic activity against HUVEC assessed as growth inhibition by NCI antiangiogenesis assay Antiangiogenic activity against HUVEC assessed as growth inhibition by NCI antiangiogenesis assay	[PMID: 19084416]
MCF7	IC₅₀	≥ 50 μM Compound: TNP470	Antiproliferative activity human MCF7 cells after 20 hrs by MTT assay Antiproliferative activity human MCF7 cells after 20 hrs by MTT assay	[PMID: 20149494]
P388	IC₅₀	> 10 ng/mL Compound: 7	Tested in vitro for its ability to inhibit cell proliferation of murine leukemia P388D1 cells measured calorimetrically by MTT method Tested in vitro for its ability to inhibit cell proliferation of murine leukemia P388D1 cells measured calorimetrically by MTT method	[PMID: 10636239]
SW 1116	IC₅₀	5.54 μM Compound: TNP-470	Antiproliferative activity against human SW1116 cells after 24 hrs by MTT assay Antiproliferative activity against human SW1116 cells after 24 hrs by MTT assay	[PMID: 21925884]

体外実験

No significant difference of apoptotic cell numbers is observed between cells treated with TNP-470 and the controls. The IC₅₀s of TNP-470 are 16.86±0.9 μg/mL, 3.16±0.6 μg/mL and 1.78±0.8 μg/mL for KKU-M213 cells at 24, 48 and 72 h, respectively. The results show that TNP-470 significantly reduces the number of migrated cells and invaded cells as compared with the vehicle treated group. TNP-470 decreases the migrated cells of KKU-M213 to 26% and of KKU-M214 to 11% (P<0.01). Similarly, TNP-470 also significantly affects cell invasion, the number of invaded cells is reduced to 25% in KKU-M213 (P<0.01) and to 15% in KKU-M214 (P<0.01). The relative expressions of MMP2, MMP9 and c-MYC in TNP-470 treated cells are significantly suppressed compared to the vehicle treated cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

TNP-470 attenuates (P<0.05) liver lipid accumulation compared to high fat fed (HFF) mice. By day 5, TNP-470 treated mice consume significantly less grams of high fat food than vehicle treated HFF mice. By day 15 of treatment, TNP-470 mice are consuming an equivalent number of calories to that of chow fed mice, despite the provision of high fat diet. Treatment with TNP-470 increases (P<0.05) expression of adipose tissue LPL mRNA, compare to chow-fed and high-fat fed controls. TNP-470 decreases energy intake and increases energy expenditure^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

臨床実験

分子量

401.88

Synonyms

AGM-1470

分子式

C₁₉H₂₈ClNO₆

CAS 番号

129298-91-5

Appearance

Solid-Liquid Mixture

Color

Colorless to off-white

SMILES

C[C@]1([C@@H](C/C=C(C)/C)O1)[C@]([C@@H]2OC)([H])[C@]3(CC[C@H]2OC(NC(CCl)=O)=O)CO3

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

溶剤 & 溶解度

体外:

DMSO : 100 mg/mL (248.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4883 mL	12.4415 mL	24.8830 mL
5 mM	0.4977 mL	2.4883 mL	4.9766 mL
10 mM	0.2488 mL	1.2442 mL	2.4883 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (12.44 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (12.44 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (12.44 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.87%

Select Batch:

データシート (275 KB) SDS (393 KB)

COA (247 KB) HNMR (141 KB) RP-HPLC (243 KB) LCMS (111 KB) OR (251 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Kidoikhammouan S, et al. TNP-470, a methionine aminopeptidase-2 inhibitor, inhibits cell proliferation, migration and invasion of human cholangiocarcinoma cells in vitro. Asian Pac J Cancer Prev. 2012;13 Suppl:155-60. [Content Brief]

[2]. White HM, et al. The angiogenic inhibitor TNP-470 decreases caloric intake and weight gain in high-fat fed mice. Obesity (Silver Spring). 2012 Oct;20(10):2003-9. [Content Brief]

細胞実験 ^[1]	MTT assays are applied to test cell viability. In brief, 3×10³ cells per well are seeded in a 96-well plate and incubated with various concentration of TNP-470 for 24, 48, and 72 h at 37°C, 5% CO₂. For comparison, cells cultured in the absence of TNP-470 are used as a control. After an incubation period, 10 μL MTT (0.5 mg/mL final concentration) is added to each well. After 4 h of additional incubation, 100 μL of 0.01 N HCl in isopropanol is added to dissolve the crystals. Absorption at 570 nm is determined by ELISA plate reader^[1]. MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。
動物実験 ^[2]	Individually housed, 4 wk old male C57BL/6 mice are used in this study. After a 1 wk acclimation period, mice are randomly allocated to receive either standard chow diet or high-fat diet for 6.5 wk. Throughout the high-fat feeding period the mice are treated with TNP-470 at a dose of 20 mg/kg body weight, injected subcutaneously every other day (TNP; n=7) or a vehicle injection of an equivalent volume (HFF controls; n=7). Vehicle injections contain 3% ethanol in phosphate-buffered saline. Chow-fed control mice (chow; n=8) are sham injected. Mice are fed ad libitum with food replaced every 2 or 3 days. Body weights are collected three times per week. After 6.5 wk of feeding, animals are fasted for 16-h and sacrificed. Final body, liver, and epididymal adipose tissue weights are measured. Liver and adipose tissue samples are frozen in liquid nitrogen and stored at -80°C for subsequent analysis^[2]. MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。
参考文献	[1]. Kidoikhammouan S, et al. TNP-470, a methionine aminopeptidase-2 inhibitor, inhibits cell proliferation, migration and invasion of human cholangiocarcinoma cells in vitro. Asian Pac J Cancer Prev. 2012;13 Suppl:155-60. [Content Brief] [2]. White HM, et al. The angiogenic inhibitor TNP-470 decreases caloric intake and weight gain in high-fat fed mice. Obesity (Silver Spring). 2012 Oct;20(10):2003-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4883 mL	12.4415 mL	24.8830 mL	62.2076 mL
	5 mM	0.4977 mL	2.4883 mL	4.9766 mL	12.4415 mL
	10 mM	0.2488 mL	1.2442 mL	2.4883 mL	6.2208 mL
	15 mM	0.1659 mL	0.8294 mL	1.6589 mL	4.1472 mL
	20 mM	0.1244 mL	0.6221 mL	1.2442 mL	3.1104 mL
	25 mM	0.0995 mL	0.4977 mL	0.9953 mL	2.4883 mL
	30 mM	0.0829 mL	0.4147 mL	0.8294 mL	2.0736 mL
	40 mM	0.0622 mL	0.3110 mL	0.6221 mL	1.5552 mL
	50 mM	0.0498 mL	0.2488 mL	0.4977 mL	1.2442 mL
	60 mM	0.0415 mL	0.2074 mL	0.4147 mL	1.0368 mL
	80 mM	0.0311 mL	0.1555 mL	0.3110 mL	0.7776 mL
	100 mM	0.0249 mL	0.1244 mL	0.2488 mL	0.6221 mL

TNP-470 Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TNP-470129298-91-5AGM-1470TNP470TNP 470AGM1470AGM 1470AminopeptidaseInhibitorinhibitorinhibit

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TNP-470 (Synonyms: AGM-1470)

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Aminopeptidase アイソフォーム固有の製品をすべて表示:

生物活性

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純度とドキュメンテーション

参考文献

カスタマーレビュー

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

TNP-470 Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

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TNP-470 (Synonyms: AGM-1470)

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TNP-470 関連抗体

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

Aminopeptidase アイソフォーム固有の製品をすべて表示:

生物活性

プロトコル

純度とドキュメンテーション

参考文献

カスタマーレビュー

TNP-470 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

TNP-470 Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

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