2. Metabolic Enzyme/Protease
  3. Aminopeptidase
  4. TNP-470

TNP-470 (Synonyms: AGM-1470)

製品番号: HY-101932 純度: ≥99.0%
取扱説明書

TNP-470 is a methionine aminopeptidase-2 inhibitor and also an angiogenesis inhibitor.

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TNP-470 構造式

TNP-470 構造式

CAS 番号 : 129298-91-5

容量 価格（税別） 在庫状況 数量
Solution
10 mM * 1 mL in DMSO USD 309 在庫あり
Estimated Time of Arrival: December 31
Liquid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 309 在庫あり
Estimated Time of Arrival: December 31
Liquid
5 mg USD 350 在庫あり
Estimated Time of Arrival: December 31
10 mg USD 550 在庫あり
Estimated Time of Arrival: December 31
50 mg   お問い合わせ  
100 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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製品説明

TNP-470 is a methionine aminopeptidase-2 inhibitor and also an angiogenesis inhibitor.

IC50 & Target

methionine aminopeptidase-2[1], angiogenesis[2]
体外実験

No significant difference of apoptotic cell numbers is observed between cells treated with TNP-470 and the controls. The IC50s of TNP-470 are 16.86±0.9 µg/mL, 3.16±0.6 µg/mL and 1.78±0.8 µg/mL for KKU-M213 cells at 24, 48 and 72 h, respectively. The results show that TNP-470 significantly reduces the number of migrated cells and invaded cells as compare with the vehicle treated group. TNP-470 decreases the migrated cells of KKU-M213 to 26% and of KKU-M214 to 11% (P<0.01). Similarly, TNP-470 also significantly affects cell invasion, the number of invaded cells is reduced to 25% in KKU-M213 (P<0.01) and to 15% in KKU-M214 (P<0.01). The relative expressions of MMP2, MMP9 and c-MYC in TNP-470 treated cells are significantly suppressed compare to the vehicle treated cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内実験

Treatment with TNP-470 attenuates (P<0.05) liver lipid accumulation compare to high fat fed (HFF) mice. By day 5, TNP-470 treated mice consume significantly less grams of high fat food than vehicle treated HFF mice. By day 15 of treatment, TNP-470 mice are consuming an equivalent number of calories to that of chow fed mice, despite the provision of high fat diet. Treatment with TNP-470 increases (P<0.05) expression of adipose tissue LPL mRNA, compare to chow-fed and high-fat fed controls. TNP-470 decreases energy intake and increases energy expenditure[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
臨床実験
分子量

401.88

分子式

C₁₉H₂₈ClNO₆
CAS 番号
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
溶剤 & 溶解度
体外: 

DMSO : 100 mg/mL (248.83 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4883 mL 12.4415 mL 24.8830 mL
5 mM 0.4977 mL 2.4883 mL 4.9766 mL
10 mM 0.2488 mL 1.2442 mL 2.4883 mL
*Please refer to the solubility information to select the appropriate solvent.
体内:
  • 1.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (6.22 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (6.22 mM); Clear solution

*All of the co-solvents are provided by MCE.
参考文献
細胞実験
[1]

MTT assays are applied to test cell viability. In brief, 3×103 cells per well are seeded in a 96-well plate and incubated with various concentration of TNP-470 for 24, 48, and 72 h at 37°C, 5% CO2. For comparison, cells cultured in the absence of TNP-470 are used as a control. After an incubation period, 10 μL MTT (0.5 mg/mL final concentration) is added to each well. After 4 h of additional incubation, 100 μL of 0.01 N HCl in isopropanol is added to dissolve the crystals. Absorption at 570 nm is determined by ELISA plate reader[1].

MCE はこれらの方法の精度を確認していません。 こちらは参照専用です。
動物実験
[2]

Individually housed, 4 wk old male C57BL/6 mice are used in this study. After a 1 wk acclimation period, mice are randomly allocated to receive either standard chow diet or high-fat diet for 6.5 wk. Throughout the high-fat feeding period the mice are treated with TNP-470 at a dose of 20 mg/kg body weight, injected subcutaneously every other day (TNP; n=7) or a vehicle injection of an equivalent volume (HFF controls; n=7). Vehicle injections contain 3% ethanol in phosphate-buffered saline. Chow-fed control mice (chow; n=8) are sham injected. Mice are fed ad libitum with food replaced every 2 or 3 days. Body weights are collected three times per week. After 6.5 wk of feeding, animals are fasted for 16-h and sacrificed. Final body, liver, and epididymal adipose tissue weights are measured. Liver and adipose tissue samples are frozen in liquid nitrogen and stored at -80°C for subsequent analysis[2].

MCE はこれらの方法の精度を確認していません。 こちらは参照専用です。
参考文献

純度: ≥99.0%

COA (246 KB)

取扱説明書 (2659 KB)
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Keywords:

TNP-470129298-91-5AGM-1470TNP470TNP 470AGM1470AGM 1470AminopeptidaseInhibitorinhibitorinhibit

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製品名:
TNP-470
製品番号:
HY-101932
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