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Thionin acetate  (Synonyms: Thionine acetate)

Cat. No.: HY-D0955 Purity: 93.00%
Handling Instructions Technical Support

Thionin acetate (Thionine acetate) is a compound present in the seeds, stems, roots, and leaves of many plant species with antibacterial activity. Thionin acetate is a metachromatic cationic histological dye widely used in biological staining.

For research use only. We do not sell to patients.

Thionin acetate

Thionin acetate Chemical Structure

CAS No. : 78338-22-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
500 mg In-stock
1 g In-stock
5 g   Get quote  
10 g   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Thionin acetate (Thionine acetate) is a compound present in the seeds, stems, roots, and leaves of many plant species with antibacterial activity. Thionin acetate is a metachromatic cationic histological dye widely used in biological staining[1][2].

Cellular Effect
Cell Line Type Value Description References
Huh-7 CC50
4.67 μg/mL
Compound: 20
Cytotoxic concentration of compound HC40 cells determined by using MTT assay
Cytotoxic concentration of compound HC40 cells determined by using MTT assay
[PMID: 10866369]
Huh-7 IC50
2.86 μg/mL
Compound: 20
Anti-Hepatitis C Viral IRES activity of compound by measured as Fluci signal in HC40 cells
Anti-Hepatitis C Viral IRES activity of compound by measured as Fluci signal in HC40 cells
[PMID: 10866369]
In Vitro

Thionin acetate can be used for tissue staining, such as staining brainstem slices. Thionin acetate can assist in observing and analyzing the brainstem structure, determining the location of the preB?tC and the characteristics of related regions[2].
The brief steps of Thionin acetate staining are as follows: First, the tissue sections are sequentially treated with 70% ethanol, 50% ethanol, and distilled water, then immersed in the Thionin acetate solution. Subsequently, they are washed successively with distilled water and ethanol of different concentrations, and finally cleared in xylene to complete the staining for observation and analysis[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

287.34

Formula

C14H13N3O2S

CAS No.
Appearance

Solid

Color

Green to dark green

SMILES

NC1=CC2=[S+]C3=C(C=CC(N)=C3)N=C2C=C1.CC([O-])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 41.67 mg/mL (145.02 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4802 mL 17.4010 mL 34.8020 mL
5 mM 0.6960 mL 3.4802 mL 6.9604 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 93.00%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.4802 mL 17.4010 mL 34.8020 mL 87.0049 mL
5 mM 0.6960 mL 3.4802 mL 6.9604 mL 17.4010 mL
10 mM 0.3480 mL 1.7401 mL 3.4802 mL 8.7005 mL
15 mM 0.2320 mL 1.1601 mL 2.3201 mL 5.8003 mL
20 mM 0.1740 mL 0.8700 mL 1.7401 mL 4.3502 mL
25 mM 0.1392 mL 0.6960 mL 1.3921 mL 3.4802 mL
30 mM 0.1160 mL 0.5800 mL 1.1601 mL 2.9002 mL
40 mM 0.0870 mL 0.4350 mL 0.8700 mL 2.1751 mL
50 mM 0.0696 mL 0.3480 mL 0.6960 mL 1.7401 mL
60 mM 0.0580 mL 0.2900 mL 0.5800 mL 1.4501 mL
80 mM 0.0435 mL 0.2175 mL 0.4350 mL 1.0876 mL
100 mM 0.0348 mL 0.1740 mL 0.3480 mL 0.8700 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Thionin acetate
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