Tolazoline hydrochloride
Based on 1 Customer Validation
Tolazoline hydrochloride (Imidaline hydrochloride) is an α-adrenergic receptor antagonist. Tolazoline hydrochloride inhibits Noradrenaline (HY-13715)-induced cell contraction, modulates vascular resistance, increases arterial pressure, and reverses bradycardia and tachypnea. Tolazoline hydrochloride can be used to study erectile dysfunction, α2-adrenergic receptor agonist-related poisoning, and skin vascular disease research.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 59-97-2
- Formula: C10H13ClN2
- Molecular Weight:196.68
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Tolazoline (1 μM; 30 s) hydrochloride inhibits the Noradrenaline (HY-13715)-induced contraction of isolated smooth muscle cells from human corpus cavernosum[3].
Tolazoline hydrochloride inhibits the specific binding of 3H-DHE to smooth muscle cells from human corpus cavernosum with a lower affinity (IC50 = 0.1 μM)[3].
Tolazoline (10 nM-1 mM) hydrochloride dose-dependently reduces vascular resistance in isolated porcine perfused skin flaps precontracted with Norepinephrine (EC50 = 0.288 μM)[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Tolazoline hydrochloride (0.5 mg/kg; iv; injected into the femoral vein) causes an increase in arterial blood pressure in cats treated with Reserpine (HY-N0480) and anesthetized with Pentobarbitone sodium[6].
Tolazoline (2 mg/kg; i.v., once a week for 3 weeks) hydrochloride shortens the recumbency, head droop, reverses bradycardia and tachypnea in sheep treated with Xylazine[7].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 59-97-2
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Appearance Solid
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Molecular Weight 196.68
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Formula C10H13ClN2
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Color White to off-white
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SMILES
[H]Cl.C1(CC2=CC=CC=C2)=NCCN1
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Synonyms
Imidaline hydrochloride; NSC35110 hydrochloride
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : ≥ 50 mg/mL (254.22 mM)
DMSO : 33.33 mg/mL (169.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.71 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (12.71 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (508.44 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Megens AA, et al. Further validation of in vivo and in vitro pharmacological procedures for assessing the alpha 2/alpha 1-selectivity of test compounds: (1). Alpha-adrenoceptor antagonists. Eur J Pharmacol. 1986 Sep 23;129(1-2):49-55. [Content Brief]
[2]. Schwartz DD, et al. Selectivity of atipamezole, yohimbine and tolazoline for alpha-2 adrenergic receptor subtypes: implications for clinical reversal of alpha-2 adrenergic receptor mediated sedation in sheep. J Vet Pharmacol Ther. 1998 Oct;21(5):342-7. [Content Brief]
[3]. Costa P, et al. Adrenergic receptors on smooth muscle cells isolated from human penile corpus cavernosum. J Urol. 1993 Sep;150(3):859-63. [Content Brief]
[4]. Rogers RA, et al. Pharmacologic modulation of the cutaneous vasculature in the isolated perfused porcine skin flap. J Pharm Sci. 1994 Dec;83(12):1682-9. [Content Brief]
[5]. Lei L, et al. Activation of L-type calcium channel by tolazoline derivatives: role of isothiocyanate moiety. J Cardiovasc Pharmacol. 1998 May;31(5):721-33. [Content Brief]
[6]. BENFEY BG, et al. VASOCONSTRICTOR ACTION OF TOLAZOLINE. Br J Pharmacol Chemother. 1964 Feb;22(1):66-71. [Content Brief]
[7]. Hsu WH, et al. Effects of tolazoline and yohimbine on xylazine-induced central nervous system depression, bradycardia, and tachypnea in sheep. J Am Vet Med Assoc. 1987 Feb 15;190(4):423-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 5.0844 mL | 25.4220 mL | 50.8440 mL | 127.1100 mL |
| 5 mM | 1.0169 mL | 5.0844 mL | 10.1688 mL | 25.4220 mL | |
| 10 mM | 0.5084 mL | 2.5422 mL | 5.0844 mL | 12.7110 mL | |
| 15 mM | 0.3390 mL | 1.6948 mL | 3.3896 mL | 8.4740 mL | |
| 20 mM | 0.2542 mL | 1.2711 mL | 2.5422 mL | 6.3555 mL | |
| 25 mM | 0.2034 mL | 1.0169 mL | 2.0338 mL | 5.0844 mL | |
| 30 mM | 0.1695 mL | 0.8474 mL | 1.6948 mL | 4.2370 mL | |
| 40 mM | 0.1271 mL | 0.6356 mL | 1.2711 mL | 3.1778 mL | |
| 50 mM | 0.1017 mL | 0.5084 mL | 1.0169 mL | 2.5422 mL | |
| 60 mM | 0.0847 mL | 0.4237 mL | 0.8474 mL | 2.1185 mL | |
| 80 mM | 0.0636 mL | 0.3178 mL | 0.6356 mL | 1.5889 mL | |
| 100 mM | 0.0508 mL | 0.2542 mL | 0.5084 mL | 1.2711 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Tolazoline hydrochloride Related Classifications
HY-A0066A Related Classifications
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