L-Glutamic acid γ-(3-carboxy-4-nitroanilide) ammonium salt
Based on 1 Customer Validation
L-Glutamic acid γ-(3-carboxy-4-nitroanilide) ammonium salt is a donor substrate of gamma-glutamyltransferase (GGT) that can be used to measure GGT activity.
For research use only. We do not sell to patients.
- Purity: 99.76%
- CAS No.: 63699-78-5
- Formula: C12H16N4O7
- Molecular Weight:328.28
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
GGT Activity Assay: Purified enzyme Is incubated with L-Glutamic acid γ-(3-carboxy-4-nitroanilide) ammonium salt (Glu-cp-NA) (1 mM) and Glycylglycine (HY-D0889) (20 mM) in PBS (0.1 M, pH 8.0) at 37°C, and the increase in absorbance at 405 nm is monitored. Enzyme activity is determined using the molar absorptivity of 8800 M-1cm-1 formed by p-nitroaniline[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 63699-78-5
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Appearance Solid
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Molecular Weight 328.28
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Formula C12H16N4O7
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Color Light yellow to yellow
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SMILES
O=C(O)C1=CC(NC(CC[C@H](N)C(O)=O)=O)=CC=C1[N+]([O-])=O.N
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Synonyms
L-γ-Glutamyl-3-carboxy-4-nitroanilide ammonium salt
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : ≥ 30 mg/mL (91.39 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 7.14 mg/mL (21.75 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (273 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Shaw LM, et al. Gamma-Glutamyltransferase: kinetic properties and assay conditions when gamma-glutamyl-4-nitroanilide and its 3-carboxy derivative are used as donor substrates. Clin Chem. 1977 Jan;23(1):79-85. [Content Brief]
[2]. Angeli V, et al. A kinetic study of gamma-glutamyltransferase (GGT)-mediated S-nitrosoglutathione catabolism. Arch Biochem Biophys. 2009 Jan 15;481(2):191-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 3.0462 mL | 15.2309 mL | 30.4618 mL | 76.1545 mL |
| 5 mM | 0.6092 mL | 3.0462 mL | 6.0924 mL | 15.2309 mL | |
| 10 mM | 0.3046 mL | 1.5231 mL | 3.0462 mL | 7.6155 mL | |
| 15 mM | 0.2031 mL | 1.0154 mL | 2.0308 mL | 5.0770 mL | |
| 20 mM | 0.1523 mL | 0.7615 mL | 1.5231 mL | 3.8077 mL | |
| 25 mM | 0.1218 mL | 0.6092 mL | 1.2185 mL | 3.0462 mL | |
| 30 mM | 0.1015 mL | 0.5077 mL | 1.0154 mL | 2.5385 mL | |
| 40 mM | 0.0762 mL | 0.3808 mL | 0.7615 mL | 1.9039 mL | |
| 50 mM | 0.0609 mL | 0.3046 mL | 0.6092 mL | 1.5231 mL | |
| 60 mM | 0.0508 mL | 0.2538 mL | 0.5077 mL | 1.2692 mL | |
| 80 mM | 0.0381 mL | 0.1904 mL | 0.3808 mL | 0.9519 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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