AASS-IN-1
Based on 1 Customer Validation
AASS-IN-1 is a 2-aminoadipic semialdehyde synthase (AASS) inhibitor with a human IC50 of 142 µM. AASS-IN-1 inhibits AASS activity by binding to the LOR domain of AASS. AASS-IN-1 can be used for the research of glutaric aciduria type 1.
For research use only. We do not sell to patients.
- Purity: 98.40%
- Formula: C15H20ClN3O2
- Molecular Weight:309.79
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
IC50：142 μM (AASS)[1]
AASS-IN-1 (S-105) (300 µM) modestly inhibits full-length human AASS-Myc-DDK (IC50 = 142 µM) and recombinant isolated human LOR domain (IC50 = 313 µM), while its R-enantiomer lacks significant inhibitory activity[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 309.79
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Formula C15H20ClN3O2
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Color Off-white to yellow
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SMILES
O=C1NC2=C(CC1)C=C(C=C2)C(N3[C@@H](CCC3)CN)=O.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (322.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 100 mg/mL (322.80 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.07 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 3.2280 mL | 16.1400 mL | 32.2799 mL | 80.6998 mL |
| 5 mM | 0.6456 mL | 3.2280 mL | 6.4560 mL | 16.1400 mL | |
| 10 mM | 0.3228 mL | 1.6140 mL | 3.2280 mL | 8.0700 mL | |
| 15 mM | 0.2152 mL | 1.0760 mL | 2.1520 mL | 5.3800 mL | |
| 20 mM | 0.1614 mL | 0.8070 mL | 1.6140 mL | 4.0350 mL | |
| 25 mM | 0.1291 mL | 0.6456 mL | 1.2912 mL | 3.2280 mL | |
| 30 mM | 0.1076 mL | 0.5380 mL | 1.0760 mL | 2.6900 mL | |
| 40 mM | 0.0807 mL | 0.4035 mL | 0.8070 mL | 2.0175 mL | |
| 50 mM | 0.0646 mL | 0.3228 mL | 0.6456 mL | 1.6140 mL | |
| 60 mM | 0.0538 mL | 0.2690 mL | 0.5380 mL | 1.3450 mL | |
| 80 mM | 0.0403 mL | 0.2017 mL | 0.4035 mL | 1.0087 mL | |
| 100 mM | 0.0323 mL | 0.1614 mL | 0.3228 mL | 0.8070 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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