1. Metabolic Enzyme/Protease
  2. Proteasome
  3. Ac-ANW-AMC

Ac-ANW-AMC is a fluorogenic substrate for immunoproteasome. Ac-ANW-AMC can be used to measure β5i activity (Ex=345 nm, Em=445 nm).

For research use only. We do not sell to patients.

Ac-ANW-AMC Chemical Structure

Ac-ANW-AMC Chemical Structure

CAS No. : 2357123-49-8

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Description

Ac-ANW-AMC is a fluorogenic substrate for immunoproteasome. Ac-ANW-AMC can be used to measure β5i activity (Ex=345 nm, Em=445 nm)[1][2].

IC50 & Target

β5i[1]

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[2].
1. For the proteasome activity assay, cells are lysed in the proteasome activity lysis buffer.
2. Cells are homogenized by passing the lysates 15 times through a 26G×1/2″ needle attached to a 1-mL syringe.
3. The lysates are then centrifuged at 12,000 rpm for 15 min. Protein concentrations of the samples are determined using the Bradford assay.
4. Proteasome activity is determined using model peptide substrates by measuring free Ac-ANW-AMC fluorescence on a TECAN infinite m200 fluorometer.
5. The fluorescence unquenched after hydrolysis by proteasomes is monitored every three minutes at 345 nm excitation and 445nm emission wavelengths at 30°C.
Note: Each sample is assayed in triplicate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

588.61

Formula

C30H32N6O7

CAS No.
Emission (Em)

460

Excitation (Ex)

380

SMILES

O=C1OC2=CC=C(C=C2C(C)=C1)NC([C@@H](NC([C@H](CC(N)=O)NC([C@H](C)NC(C)=O)=O)=O)CC3=CNC4=CC=CC=C34)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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Ac-ANW-AMC Related Classifications

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Ac-ANW-AMC
Cat. No.:
HY-D1705
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