Ac-ANW-AMC
Based on 1 Customer Validation
Ac-ANW-AMC is a fluorogenic substrate for immunoproteasome. Ac-ANW-AMC can be used to measure β5i activity (Ex=345 nm, Em=445 nm).
For research use only. We do not sell to patients.
- Purity: 98.88%
- CAS No.: 2357123-49-8
- Formula: C30H32N6O7
- Molecular Weight:588.61
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
β5i[1]
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
proteasome activity assay:
1. Cells are lysed in the proteasome activity lysis buffer.
2. Cells are homogenized by passing the lysates 15 times through a 26G×1/2″ needle attached to a 1-mL syringe.
3. The lysates are then centrifuged at 12,000 rpm for 15 min. Protein concentrations of the samples are determined using the Bradford assay.
4. Proteasome activity is determined using model peptide substrates by measuring free Ac-ANW-AMC fluorescence on a TECAN infinite m200 fluorometer.
5. The fluorescence unquenched after hydrolysis by proteasomes is monitored every three minutes at 345 nm excitation and 445nm emission wavelengths at 30°C.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
460
380
Chemical Information
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CAS No. 2357123-49-8
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Appearance Solid
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Molecular Weight 588.61
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Formula C30H32N6O7
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Color White to off-white
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SMILES
O=C1OC2=CC=C(C=C2C(C)=C1)NC([C@@H](NC([C@H](CC(N)=O)NC([C@H](C)NC(C)=O)=O)=O)CC3=CNC4=CC=CC=C34)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : ≥ 100 mg/mL (169.89 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6989 mL | 8.4946 mL | 16.9892 mL | 42.4729 mL |
| 5 mM | 0.3398 mL | 1.6989 mL | 3.3978 mL | 8.4946 mL | |
| 10 mM | 0.1699 mL | 0.8495 mL | 1.6989 mL | 4.2473 mL | |
| 15 mM | 0.1133 mL | 0.5663 mL | 1.1326 mL | 2.8315 mL | |
| 20 mM | 0.0849 mL | 0.4247 mL | 0.8495 mL | 2.1236 mL | |
| 25 mM | 0.0680 mL | 0.3398 mL | 0.6796 mL | 1.6989 mL | |
| 30 mM | 0.0566 mL | 0.2832 mL | 0.5663 mL | 1.4158 mL | |
| 40 mM | 0.0425 mL | 0.2124 mL | 0.4247 mL | 1.0618 mL | |
| 50 mM | 0.0340 mL | 0.1699 mL | 0.3398 mL | 0.8495 mL | |
| 60 mM | 0.0283 mL | 0.1416 mL | 0.2832 mL | 0.7079 mL | |
| 80 mM | 0.0212 mL | 0.1062 mL | 0.2124 mL | 0.5309 mL | |
| 100 mM | 0.0170 mL | 0.0849 mL | 0.1699 mL | 0.4247 mL |
Ac-ANW-AMC Related Classifications
HY-D1705 Related Classifications
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