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  3. Fluorescent Dye
  4. Ala-Phe-Lys-AMC

Ala-Phe-Lys-AMC is a fluorogenic substrate, which can be used for residual plasmin activity assay.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Ala-Phe-Lys-AMC

Ala-Phe-Lys-AMC Chemical Structure

CAS No. : 120928-02-1

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Based on 1 publication(s) in Google Scholar

Ala-Phe-Lys-AMC Related Antibodies

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Description

Ala-Phe-Lys-AMC is a fluorogenic substrate, which can be used for residual plasmin activity assay[1].

Molecular Weight

521.61

Formula

C28H35N5O5
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Ala-Phe-Lys-{AMC}
Sequence Shortening

AFK-{AMC}
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (191.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9171 mL 9.5857 mL 19.1714 mL
5 mM 0.3834 mL 1.9171 mL 3.8343 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9171 mL 9.5857 mL 19.1714 mL 47.9285 mL
5 mM 0.3834 mL 1.9171 mL 3.8343 mL 9.5857 mL
10 mM 0.1917 mL 0.9586 mL 1.9171 mL 4.7929 mL
15 mM 0.1278 mL 0.6390 mL 1.2781 mL 3.1952 mL
20 mM 0.0959 mL 0.4793 mL 0.9586 mL 2.3964 mL
25 mM 0.0767 mL 0.3834 mL 0.7669 mL 1.9171 mL
30 mM 0.0639 mL 0.3195 mL 0.6390 mL 1.5976 mL
40 mM 0.0479 mL 0.2396 mL 0.4793 mL 1.1982 mL
50 mM 0.0383 mL 0.1917 mL 0.3834 mL 0.9586 mL
60 mM 0.0320 mL 0.1598 mL 0.3195 mL 0.7988 mL
80 mM 0.0240 mL 0.1198 mL 0.2396 mL 0.5991 mL
100 mM 0.0192 mL 0.0959 mL 0.1917 mL 0.4793 mL
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Ala-Phe-Lys-AMC Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ala-Phe-Lys-AMC120928-02-1Fluorescent DyeInhibitorinhibitorinhibit

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