Angiogenesis agent 1
Based on 1 Customer Validation
Angiogenesis agent 1 (compound C-31) is a salidroside-derivated glycoside analogue. Angiogenesis agent 1 is an activator of the HIF-1α pathway. Angiogenesis agent 1 has the potential for the research of diabetic hind limb ischemia.
For research use only. We do not sell to patients.
- Purity: 99.39%
- CAS No.: 2765218-28-6
- Formula: C20H24O7
- Molecular Weight:376.40
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| C2C12 | EC50 |
12.7 μM
Compound: C-30
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Angiogenic activity in mouse C2C12 cells assessed as reversal of hyperglycemia-induced reduction in total cell number measured after 12 to 36 hrs
Angiogenic activity in mouse C2C12 cells assessed as reversal of hyperglycemia-induced reduction in total cell number measured after 12 to 36 hrs
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[PMID: 34939794] |
| C2C12 | EC50 |
17.32 μM
Compound: C-30
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Angiogenic activity in mouse C2C12 cells assessed as reversal of hyperglycemia-induced reduction in cell number by measuring cell proliferation measured after 12 to 36 hrs
Angiogenic activity in mouse C2C12 cells assessed as reversal of hyperglycemia-induced reduction in cell number by measuring cell proliferation measured after 12 to 36 hrs
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[PMID: 34939794] |
Chemical Information
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CAS No. 2765218-28-6
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Appearance Solid
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Molecular Weight 376.40
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Formula C20H24O7
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Color White to off-white
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SMILES
O[C@@H]1[C@H]([C@@H]([C@H](O[C@H]1OC2=CC(CC3=CC=C(C=C3)OC)=CC=C2)CO)O)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (265.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (13.28 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (13.28 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6567 mL | 13.2837 mL | 26.5675 mL | 66.4187 mL |
| 5 mM | 0.5313 mL | 2.6567 mL | 5.3135 mL | 13.2837 mL | |
| 10 mM | 0.2657 mL | 1.3284 mL | 2.6567 mL | 6.6419 mL | |
| 15 mM | 0.1771 mL | 0.8856 mL | 1.7712 mL | 4.4279 mL | |
| 20 mM | 0.1328 mL | 0.6642 mL | 1.3284 mL | 3.3209 mL | |
| 25 mM | 0.1063 mL | 0.5313 mL | 1.0627 mL | 2.6567 mL | |
| 30 mM | 0.0886 mL | 0.4428 mL | 0.8856 mL | 2.2140 mL | |
| 40 mM | 0.0664 mL | 0.3321 mL | 0.6642 mL | 1.6605 mL | |
| 50 mM | 0.0531 mL | 0.2657 mL | 0.5313 mL | 1.3284 mL | |
| 60 mM | 0.0443 mL | 0.2214 mL | 0.4428 mL | 1.1070 mL | |
| 80 mM | 0.0332 mL | 0.1660 mL | 0.3321 mL | 0.8302 mL | |
| 100 mM | 0.0266 mL | 0.1328 mL | 0.2657 mL | 0.6642 mL |
Angiogenesis agent 1 Related Classifications
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