Antitumor agent-143
Antitumor agent-143 (compound 2c) is an antitumor agent that blocks cell proliferation of A549 cells during the S phase and induces an early apoptosis. Antitumor agent-143 induces cell death via ferroptosis, apoptosis by a ROS-mediated mitochondrial dysfunction pathway and GSDMD-mediated pyroptosis.
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- Formule: C47H29Cl2F6IrN9P
- Masse moléculaire:1127.88
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
Antitumor agent-143 (compound 2c; 48h) inhibits the cell growth of A549, B16, HCT116 and HepG2 cells with IC50 values of 2.2 μM, 2.5 μM, 2.5 μM and 1 μM, respectively[1].
Antitumor agent-143 (compound 2c) blocks cell proliferation of A549 cells during the S phase and induces an early apoptosis[1].
Antitumor agent-143 (compound 2c) leads to elevated ROS and Ca2+ amounts. This resulted in a reduced mitochondrial membrane potential, mitochondrial permeability transition pore opening, and an increase of cytochrome c[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c nude mice (3-4 weeks) injected with A549 cells[1]
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Dosage:3.2 or 5.0 mg/kg
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Administration:Intraperitoneal injection; daily; for 7 days
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Result:Exhibited a remarkable inhibitory rate of 58.58% in restraining tumor growth.
- Somatic Cell Culture
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Flow cytometric DNA-content cell-cycle staining
- Cell Cytotoxicity Assay
- Mitochondrial membrane-potential and mitochondrial mass staining
- Apoptosis Solutions
- Ferroptosis Solutions
- MTT Cell Proliferation Assay
- Pyroptosis Solutions
- Mammalian live/dead viability and cytotoxicity staining
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- ROS/oxidative-stress fluorescent staining
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Ki-67 Immunostaining Proliferation Assay
- PCNA Immunodetection Proliferation Assay
- Protocol for Cell Counting and Cell Density Analysis
Chemical Information
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Masse moléculaire 1127.88
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Formule C47H29Cl2F6IrN9P
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SMILES
F[P-](F)(F)(F)(F)F.NC(N=C1Cl)=NC(Cl)=C1C(N2)=NC3=C2C(C=CC=[N]45)=C5C6=C3C=CC=[N]6[Ir]74([N]8=C9C(C=CC=C%10)=C%10C=C8)(C%11=C9C=CC=C%11)[N]%12=C(C%13=CC=CC=C%137)C%14=C(C=CC=C%14)C=C%12
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
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Fiche technique (276 KB)
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SDS (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Antitumor agent-143 Related Classifications
HY-161332 Related Classifications
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