Antiviral agent 86 

Antiviral agent 86 is an anti-coronavirus agent. Antiviral agent 86 acts as a binder of coronavirus non-structural protein 15 (nsp15), with a K_a value of 67 μM against human targets. Antiviral agent 86 inhibits the replication of HCoV-229E and SARS-CoV-2. Antiviral agent 86 exerts inhibitory effects at the post-entry lifecycle stage of coronaviruses in host cells and inhibits the formation of viral double-stranded RNA (dsRNA). Antiviral agent 86 exhibits an additive antiviral effect when used in combination with GS-441524. Antiviral agent 86 can be used in studies related to coronavirus infections^[1].

Antiviral agent 86 (Compound 2p) (5 days) potently inhibits the replication of HCoV-229E in HEL299 cells, with an EC₅₀ of 9.3 μM, and no cytotoxicity is detected at a concentration of 100 μM^[1].
Antiviral agent 86 (5 days) inhibits the replication of SARS-CoV-2 in A549ACE2/TMPRSS2 cells, with an EC₅₀ of 12 μM, and no cytotoxicity is detected at a concentration of 100 μM^[1].
Antiviral agent 86 (1-5 days) exhibits pan-coronavirus activity and acts on human and animal coronaviruses belonging to the α and β genera, with EC₉₀ values ranging from 2.2 μM (HCoV-OC43 in HRT-18G cells) to 30 μM (TGEV in ST cells). No cytotoxicity is detected at a concentration of 100 μM across all tested cell lines^[1].
Antiviral agent 86 (20 μM, 1 day) potently inhibits the production of viral dsRNA in HCoV-229E-infected 16HBE cells, indicating that it is capable of suppressing viral replication^[1].
Antiviral agent 86 (0.205-50 μM; 5 days) exhibits additive antiviral activity when used in combination with GS-441524 (HY-103586) in HCoV-229E-infected HEL299 cells^[1].
Antiviral agent 86 (5 days) shows reduced potency against nsp15 mutant (V42A or F230L) HCoV-229E in HEL299 cells, with its EC₉₀ values increasing to 7.7 μM and 9.4 μM, respectively^[1].
Antiviral agent 86 (6.25-200 μM; 120-second association, 360-second dissociation) binds directly to recombinant HCoV-229E nsp15 protein, with a K_D value of approximately 67 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

436.61

C₂₆H₃₂N₂O₂S

O=C(NN1C(C(C)SC12CCC(C3=CC=CC=C3)CC2)=O)C4=CC=C(C(C)(C)C)C=C4

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Van Loy B, et al. Structure-activity relationship and nsp15-dependent mechanism of spirothiazolidinone derivatives with pan-coronavirus activity. Bioorg Chem. Published online May 15, 2026.