APH-IN-1
APH-IN-1 is an Aminoglycoside phosphotransferase (APH) inhibitor with a Kd values of 63 nM against Escherichia coli APH(3′)-Ia. APH-IN-1 binds primarily to the ATP-binding pocket of APH in an ATP-competitive manner, with binding affinity enhanced by divalent metal ions (Mg2+ and Ca2+). APH-IN-1 can be used for the research of aminoglycoside-resistant bacterial infections.
For research use only. We do not sell to patients.
- Formula: C60H90N14O11S2
- Molecular Weight:1247.57
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
APH-IN-1 (Compound I-L3) (20 nM; 30 min) binds to purified Escherichia coli-derived APH(3′)-Ia with a Kd of 63 nM[1].
APH-IN-1 (20 nM; 30 min) exhibits enhanced binding to purified Escherichia coli-derived APH(3′)-Ia in the presence of divalent metal ions[1].
APH-IN-1 (20 nM) binds primarily to the ATP-binding pocket of purified Escherichia coli-derived APH(3′)-Ia, as shown by competitive displacement by ATP[1].
APH-IN-1 (0.1-5.00 μM; 1 h) inhibits the activity of purified Escherichia coli-derived APH(3′)-Ia in a concentration-dependent manner[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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Molecular Weight 1247.57
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Formula C60H90N14O11S2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Ca2+ Staining Technique
Ca2+ staining is an experimental technique that utilizes specific fluorescent probes (such as Fluo-4 AM, Fura-2, etc.) to qualitatively or quantitatively detect dynamic changes in intracellular Ca2+ concentrations; this is achieved by monitoring the changes in fluorescent signals generated when these probes bind to free intracellular calcium ions. The underlying principle relies primarily on the presence of chelating groups within the probe's molecular structure that possess high affinity for calcium ions.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
APH-IN-1 Related Classifications
HY-P11825 Related Classifications
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