Atglistatin
Based on 43 publication(s) in Google Scholar
Atglistatin is a selective adipose triglyceride lipase (ATGL) inhibitor which inhibits lipolysis with an IC50 of 0.7 μM in vitro.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 99.44%
- CAS. Nr.: 1469924-27-3
- Formel: C17H21N3O
- Molecular Weight:283.37
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
Publications Citing Use of MedChemExpress (MCE) Atglistatin
More- Signal Transduct Target Ther. 2025 Sep 26;10(1):315. [Abstract]
- Cell Metab. 2025 Sep 24:S1550-4131(25)00385-7. [Abstract]
- Cell Metab. 2022 Sep 6;34(9):1325-1341.e6. [Abstract]
- Cell Mol Immunol. 2025 Apr 7. [Abstract]
- Nat Commun. 2024 Sep 27;15(1):8273. [Abstract]
- Nat Commun. 2022 Nov 2;13(1):6577. [Abstract]
- Neuron. 2020 Jan 22;105(2):276-292.e5. [Abstract]
- Autophagy. 2026 May 27:1-27. [Abstract]
- Autophagy. 2026 Apr 27. [Abstract]
- Autophagy. 2026 Mar 11:1-18. [Abstract]
- Autophagy. 2025 Dec 24:1-19. [Abstract]
- Adv Sci (Weinh). 2025 Nov 19:e10203. [Abstract]
- J Adv Res. 2025 Aug 8:S2090-1232(25)00596-X. [Abstract]
- J Biomed Sci. 2020 Sep 1;27(1):91. [Abstract]
- Mol Ther. 2025 Feb 5;33(2):805-822. [Abstract]
- Diabetologia. 2022 Apr;65(4):705-720. [Abstract]
- Cell Death Dis. 2025 Dec 23. [Abstract]
- Cell Death Dis. 2025 Apr 14;16(1):291. [Abstract]
- Cell Death Dis. 2025 Jan 31;16(1):57. [Abstract]
- Cell Commun Signal. 2024 Nov 25;22(1):564. [Abstract]
- EMBO J. 2024 Dec;43(24):6383-6409. [Abstract]
- EMBO Mol Med. 2024 Jul;16(7):1533-1559. [Abstract]
- Brain Behav Immun. 2025 Jan:123:315-333. [Abstract]
- Cell Rep. 2024 Apr 23;43(5):114132. [Abstract]
- Liver Int. 2020 Jun;40(6):1378-1394. [Abstract]
- Glia. 2026 Feb;74(2):e70118. [Abstract]
- Invest Ophthalmol Vis Sci. 2024 Dec 2;65(14):12. [Abstract]
- Am J Pathol. 2024 Aug;194(8):1494-1510. [Abstract]
- Biochim Biophys Acta Mol Cell Biol Lipids. 2025 Aug 22;1870(8):159683. [Abstract]
- Biochim Biophys Acta Mol Cell Biol Lipids. 2021 Sep;1866(9):158988. [Abstract]
- Diabetes Metab Syndr Obes. 2025 Nov 14:18:4211-4227. [Abstract]
- Electrophoresis. 2025 Sep 23. [Abstract]
- Theriogenology. 2025 Nov:247:117570. [Abstract]
- Fish Physiol Biochem. 2021 Feb;47(1):173-188. [Abstract]
- Fish Physiol Biochem. 2017 Jun;43(3):813-822. [Abstract]
- Biochem Biophys Res Commun. 2026 May 12:822:153940. [Abstract]
- bioRxiv. 2025 Jul 21.
- SSRN. 2025 May 10.
- Research Square Preprint. 2024 Oct 18.
- Patent. US20240285557A1.
- bioRxiv. 2024 June 12.
- bioRxiv. 2023 Dec 1.
- bioRxiv. 2023 Jul 3.
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WB
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IF
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IF
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Cell Imaging/Staining
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Histological Imaging/Staining
Biologische Aktivität
IC50: 0.7 μM (ATGL)[1]
Atglistatin inhibits Triacylglycerol (TG) hydrolase activity of wild-type white adipose tissue (WAT) in a dose-dependent manner up to 78% at the highest concentration. In comparison to wild-type preparations, TG hydrolase activity in WAT lysates from ATGL-ko animals is reduced by approximately 70% and Atglistatin had only a moderate effect on the residual activity. The combined use of Atglistatin and the hormone-sensitive lipase (HSL) inhibitor Hi 76-0079 leads to an almost complete inhibition (-95%) of TG hydrolase activity of WAT which implicates that most of the non-ATGL activity can be ascribed to HSL[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 1469924-27-3
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Appearance Solid
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Molecular Weight 283.37
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Formel C17H21N3O
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Color White to yellow
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SMILES
O=C(NC1=CC(C2=CC=C(N(C)C)C=C2)=CC=C1)N(C)C
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (43)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
A two-strata energy flux system driven by a stress hormone prioritizes cardiac energetics. [Abstract]2025 Sep 26;10(1):315. PMID: 40998786 -
Cell Metab
Liver-breast communication of adipocyte-oriented exosomes drives primary mammary cancer progression. [Abstract]2025 Sep 24:S1550-4131(25)00385-7. PMID: 40997802 -
Cell Metab
The mitochondrial calcium uniporter engages UCP1 to form a thermoporter that promotes thermogenesis. [Abstract]2022 Sep 6;34(9):1325-1341.e6. PMID: 35977541 -
Cell Mol Immunol
2025 Apr 7. PMID: 40195475 -
Nat Commun
Lipid droplets sequester palmitic acid to disrupt endothelial ciliation and exacerbate atherosclerosis in male mice. [Abstract]2024 Sep 27;15(1):8273. PMID: 39333556
Atglistatin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Sep 27;15(1):8273. [Abstract]
Immunofluorescence images of HAECs treated with ATGL inhibitor (Atglistatin, 10 µM) for 12 h, followed by serum starvation for 48 h (n = 20 fields from 3 independent experiments).
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Nat Commun
Phosphorylation of 17β-hydroxysteroid dehydrogenase 13 at serine 33 attenuates nonalcoholic fatty liver disease in mice. [Abstract]2022 Nov 2;13(1):6577. PMID: 36323699
Atglistatin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Nov 2;13(1):6577. [Abstract]
Confocal micrographs showing the LDs (red) and nuclei (blue) in primary hepatocytes isolated from two genotypes treated with atglistatin, an ATGL inhibitor (ATGLi, 10 μM) for 24 h.
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Neuron
2020 Jan 22;105(2):276-292.e5. PMID: 31786011
Atglistatin purchased from MedChemExpress. Usage Cited in: Neuron. 2020 Jan 22;105(2):276-292.e5. [Abstract]
Representative images of DRG neurons in primary cultures treated with DMSO vehicle, Atglistatin (10 μM, 9-11 d). DRG neurites were visualized by Tuj1 staining. Scale bar: 400 μm.
Atglistatin purchased from MedChemExpress. Usage Cited in: Neuron. 2020 Jan 22;105(2):276-292.e5. [Abstract]
Sections of sciatic nerves from WT animals treated with Atglistatin (40mg/kg/day by intraperitoneal injection for consecutive 5 days before injury and 2 days after injury.). Axons are visualized by SCG10 staining. Scale bar: 400 μm.
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Autophagy
PRRSV NSP2 hijacks host lipophagy via a LIPE-PNPLA2-AMPK-MTOR axis to promote viral replication. [Abstract]2026 May 27:1-27. PMID: 42200529 -
Autophagy
The nuclear receptor ESRRA is a crucial regulator of acute kidney injury through inhibition of the lipophagy-ferroptosis axis. [Abstract]2026 Apr 27. PMID: 42041131 -
Autophagy
2026 Mar 11:1-18. PMID: 41810751 -
Autophagy
Mitochondrial protein COX5B orchestrates antiviral autophagy and apoptosis to restrict SRBSDV propagation in Sogatella furcifera. [Abstract]2025 Dec 24:1-19. PMID: 41358576 -
Adv Sci (Weinh)
Seipin-Mediated Lipid Droplet Formation in Cardiomyocytes Ameliorates Cardiac Ischemia/Reperfusion Injury. [Abstract]2025 Nov 19:e10203. PMID: 41255216
Atglistatin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 19:e10203. [Abstract]
Western blotting images of GRP78, Bcl2 and Bax (I, n = 6 each group) in the hearts of WT mice with or without I/R injury (30 min ischemia followed by 24 h reperfusion) with vehicle or Atglistatin treatment (50 mg/kg, i.p., 4 h before I/R surgery)
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J Adv Res
Epigenetically silenced KAT2B suppresses de novo lipogenesis through destroying HDAC5/LSD1 complex assembly in renal cell carcinoma. [Abstract]2025 Aug 8:S2090-1232(25)00596-X. PMID: 40784518 -
J Biomed Sci
PEDF promotes the repair of bone marrow endothelial cell injury and accelerates hematopoietic reconstruction after bone marrow transplantation. [Abstract]2020 Sep 1;27(1):91. PMID: 32873283 -
Mol Ther
ATGL regulates renal fibrosis by reprogramming lipid metabolism during the transition from AKI to CKD. [Abstract]2025 Feb 5;33(2):805-822. PMID: 39748508 -
Diabetologia
Glucolipotoxicity promotes the capacity of the glycerolipid/NEFA cycle supporting the secretory response of pancreatic beta cells. [Abstract]2022 Apr;65(4):705-720. PMID: 35018486 -
Cell Death Dis
Lysosomal exocytosis by macrophages as a druggable mechanism for anti-inflammatory clearance of dead adipocytes in adipose tissue. [Abstract]2025 Dec 23. PMID: 41436477 -
Cell Death Dis
A targetable antioxidant defense mechanism to EZH2 inhibitors enhances tumor cell vulnerability to ferroptosis. [Abstract]2025 Apr 14;16(1):291. PMID: 40229247 -
Cell Death Dis
Estrogen-dependent activation of TRX2 reverses oxidative stress and metabolic dysfunction associated with steatotic disease. [Abstract]2025 Jan 31;16(1):57. PMID: 39890799 -
Cell Commun Signal
N-acetylaspartate mitigates pro-inflammatory responses in microglial cells by intersecting lipid metabolism and acetylation processes. [Abstract]2024 Nov 25;22(1):564. PMID: 39587614 -
EMBO J
Rab2A-mediated Golgi-lipid droplet interactions support very-low-density lipoprotein secretion in hepatocytes. [Abstract]2024 Dec;43(24):6383-6409. PMID: 39496977 -
EMBO Mol Med
FADS1/2 control lipid metabolism and ferroptosis susceptibility in triple-negative breast cancer. [Abstract]2024 Jul;16(7):1533-1559. PMID: 38926633 -
Brain Behav Immun
Blockage of ATGL-mediated breakdown of lipid droplets in microglia alleviates neuroinflammatory and behavioural responses to lipopolysaccharides. [Abstract]2025 Jan:123:315-333. PMID: 39326768 -
Cell Rep
Adipocyte PI3K links adipostasis with baseline insulin secretion at fasting through an adipoincretin effect. [Abstract]2024 Apr 23;43(5):114132. PMID: 38656871 -
Liver Int
Targeting ferroptosis alleviates methionine-choline deficient (MCD)-diet induced NASH by suppressing liver lipotoxicity. [Abstract]2020 Jun;40(6):1378-1394. PMID: 32145145 -
Glia
RetSat Knockout Mitigates Hypoxia-Induced Microglial Activation by Enhancing Lipid Droplets Degradation. [Abstract]2026 Feb;74(2):e70118. PMID: 41388745 -
Invest Ophthalmol Vis Sci
Atg5-Mediated Lipophagy Induces Ferroptosis in Corneal Epithelial Cells in Dry Eye Disease. [Abstract]2024 Dec 2;65(14):12. PMID: 39636725 -
Am J Pathol
Adipose Triglyceride Lipase-Mediated Adipocyte Lipolysis Exacerbates Acute Pancreatitis Severity in Mouse Models and Patients. [Abstract]2024 Aug;194(8):1494-1510. PMID: 38705384 -
Biochim Biophys Acta Mol Cell Biol Lipids
Modulation of lipid droplet biogenesis by p38 MAPK and caspase-1 in docosahexaenoic acid-induced EA.hy926 endothelial cell apoptosis. [Abstract]2025 Aug 22;1870(8):159683. PMID: 40850528 -
Biochim Biophys Acta Mol Cell Biol Lipids
Lipolysis and lipophagy play individual and interactive roles in regulating triacylglycerol and cholesterol homeostasis and mitochondrial form in zebrafish. [Abstract]2021 Sep;1866(9):158988. PMID: 34111526 -
Diabetes Metab Syndr Obes
PEDF Alleviates Diabetic Renal Fibrosis by Degrading Kidney Ectopic Fat Deposition and Inhibiting Metabolic Reprogramming of Renal Tubular Epithelial Cells. [Abstract]2025 Nov 14:18:4211-4227. PMID: 41262465 -
Electrophoresis
Towards an Analytical Procedure Control Strategy for the Capillary Zone Electrophoresis Method for Monoclonal Antibodies: Alternatives for ε-Aminocaproic Acid and Triethylenetetramine. [Abstract]2025 Sep 23. PMID: 40988426 -
Theriogenology
Salidroside improves bovine embryo quality by mitigating oxidative stress and regulating mitochondrial lipid metabolism. [Abstract]2025 Nov:247:117570. PMID: 40652734 -
Fish Physiol Biochem
The reduction of lipid-sourced energy production caused by ATGL inhibition cannot be compensated by activation of HSL, autophagy, and utilization of other nutrients in fish. [Abstract]2021 Feb;47(1):173-188. PMID: 33245450 -
Fish Physiol Biochem
2017 Jun;43(3):813-822. PMID: 28058519
Atglistatin purchased from MedChemExpress. Usage Cited in: Fish Physiol Biochem. 2017 Jun;43(3):813-822. [Abstract]
Atglistatin (HY-15859) attenuates the increase in GyK induced by EPA at 12 h. GyK gene expression and enzyme activity after hypertrophic model cells treated with 50 μM HY-15859, 100 μM EPA alone, or both for 12 h.HY-15859 is added 2 h in advance.
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Biochem Biophys Res Commun
Cassiaside C alleviates IRI-induced AKI through restoring the expression of ATGL to reduce lipid droplet accumulation. [Abstract]2026 May 12:822:153940. PMID: 42134150 -
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Lösungsmittel & Löslichkeit
DMSO : ≥ 100 mg/mL (352.90 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.82 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protokoll
For MTT-based in vitro viability assays, cells are seeded at an initial density of 1×104 cells per well in 96-well plates and cultured under standard conditions for 24 hours. The next day, cells are pretreated with different concentrations of Atglistatin dissolved in DMSO or Cisplatin dissolved in dimethylformamide (DMF) as positive control for two hours. Medium is replaced by an identical fresh medium and incubated again for the indicated time-points. Thereafter cells are incubated for 3 hours with 100 μL Thiazolyl Blue Tetrazolium Bromide (MTT). The resulting violet formazan crystals are dissolved by adding 100 μL of MTT solubilization solution (0.1% NP-40, 4 mM HCl and anhydrous isopropanol). After complete dissolution of the formazan product, absorbance is measured at 595 nm using 690 nm as reference wavelength[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Mice[1]
Mice (C57Bl/6J) are used. Atglistatin is administrated orally by gavage in olive oil (200 μL) or by IP injection. For IP administration, we generated Atglistatin hydrochloride by the addition of 25 % HCl resulting in a water soluble compound. For intraperitoneal injection the inhibitor is dried, excess HCl is buffered with Tris base, and Atglistatin is dissolved in PBS containing 0.25 % Cremophor EL (pH 7.1). Atglistatin is administered orally by gavage in olive oil (1.4 mg/mouse). After 8 h tissues are collected and extracted twice using Folch procedure. Combined organic phases are concentrated, reconstituted in 500 μL chloroform and ssubjected to solid phase extraction (SPE). For SPE samples are loaded onto silica columns washed twice with 2 mL of chloroform and Atglistatin is eluted using 3 mL chloroform/methanol (99/1, v/v). Eluted samples are concentrated, dissolved in n-propanol/chloroform/methanol (8/1.3/0.6, v/v/v) and analyzed by UPLC/MS (m/z 284, MH+; SYNAPT G1 qTOF HD mass spectrometer, Waters).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Reinheit & Dokumentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.5290 mL | 17.6448 mL | 35.2896 mL | 88.2239 mL |
| 5 mM | 0.7058 mL | 3.5290 mL | 7.0579 mL | 17.6448 mL | |
| 10 mM | 0.3529 mL | 1.7645 mL | 3.5290 mL | 8.8224 mL | |
| 15 mM | 0.2353 mL | 1.1763 mL | 2.3526 mL | 5.8816 mL | |
| 20 mM | 0.1764 mL | 0.8822 mL | 1.7645 mL | 4.4112 mL | |
| 25 mM | 0.1412 mL | 0.7058 mL | 1.4116 mL | 3.5290 mL | |
| 30 mM | 0.1176 mL | 0.5882 mL | 1.1763 mL | 2.9408 mL | |
| 40 mM | 0.0882 mL | 0.4411 mL | 0.8822 mL | 2.2056 mL | |
| 50 mM | 0.0706 mL | 0.3529 mL | 0.7058 mL | 1.7645 mL | |
| 60 mM | 0.0588 mL | 0.2941 mL | 0.5882 mL | 1.4704 mL | |
| 80 mM | 0.0441 mL | 0.2206 mL | 0.4411 mL | 1.1028 mL | |
| 100 mM | 0.0353 mL | 0.1764 mL | 0.3529 mL | 0.8822 mL |